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Article

Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance

  • Authors:
    • Shuhai Sheng
    • Jinji Zhang
    • Jianzhong Ai
    • Xueli Hao
    • Ruishen Luan
  • View Affiliations / Copyright

    Affiliations: Department of Breast Cancer Surgery, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, P.R. China, Department of Oncological Surgery, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China, Department of Breast and Neck Surgery, The Third Hospital of Chengde City, Chengde, Hebei 067000, P.R. China
  • Pages: 4639-4644
    |
    Published online on: January 12, 2018
       https://doi.org/10.3892/mmr.2018.8427
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Abstract

Breast cancer tissues and adjacent tissues were collected from 32 patients who were treated in The Third Hospital of Chengde City. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that, compared with the adjacent tissues, interleukin (IL)‑23/IL‑23 receptor (R) gene expression levels were notably higher in breast cancer tissues. Furthermore, IL‑23 and IL‑23R expression levels were positively correlated with patients' tumor size, TNM stage and metastasis. Recombinant human (rh) IL‑23 (10 ng/ml) was used for the stimulation of the MCF‑7 cell line. Effects of rh IL‑23 (10 ng/ml) on cell proliferation was detected after MCF‑7 cells were incubated with rh IL‑23 for 48 h. Whether pre‑treatment with polyclonal antibody (PAb) IL‑23p19, a neutralizing antibody specific for IL‑23, may influence the effects of IL‑23 on cell behavior was also investigated. Cell proliferation assay and cell apoptosis assay were evaluated using MTT assay and flow cytometry assay, respectively. Results suggested that PAb IL‑23p19 reduced IL-23-induced cell proliferation whereas induced IL‑23 inhibited cell apoptosis. Western blot analysis was performed for the detection of molecules that may be responsible for the aforementioned changes. Results indicated that PAb IL‑23p19 treatment reduced IL‑23‑induced upregulation of B‑cell lymphoma‑2 protein expression and activation of the janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. The present results suggested that IL‑23 may be a potential prognosis marker and target for the treatment of breast cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Sheng S, Zhang J, Ai J, Hao X and Luan R: Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance. Mol Med Rep 17: 4639-4644, 2018.
APA
Sheng, S., Zhang, J., Ai, J., Hao, X., & Luan, R. (2018). Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance. Molecular Medicine Reports, 17, 4639-4644. https://doi.org/10.3892/mmr.2018.8427
MLA
Sheng, S., Zhang, J., Ai, J., Hao, X., Luan, R."Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance". Molecular Medicine Reports 17.3 (2018): 4639-4644.
Chicago
Sheng, S., Zhang, J., Ai, J., Hao, X., Luan, R."Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance". Molecular Medicine Reports 17, no. 3 (2018): 4639-4644. https://doi.org/10.3892/mmr.2018.8427
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng S, Zhang J, Ai J, Hao X and Luan R: Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance. Mol Med Rep 17: 4639-4644, 2018.
APA
Sheng, S., Zhang, J., Ai, J., Hao, X., & Luan, R. (2018). Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance. Molecular Medicine Reports, 17, 4639-4644. https://doi.org/10.3892/mmr.2018.8427
MLA
Sheng, S., Zhang, J., Ai, J., Hao, X., Luan, R."Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance". Molecular Medicine Reports 17.3 (2018): 4639-4644.
Chicago
Sheng, S., Zhang, J., Ai, J., Hao, X., Luan, R."Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance". Molecular Medicine Reports 17, no. 3 (2018): 4639-4644. https://doi.org/10.3892/mmr.2018.8427
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