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Article Open Access

Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis

  • Authors:
    • Bobin Mi
    • Guohui Liu
    • Wu Zhou
    • Huijuan Lv
    • Yi Liu
    • Jing Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Rheumatology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710000, P.R. China
    Copyright: © Mi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4467-4473
    |
    Published online on: January 15, 2018
       https://doi.org/10.3892/mmr.2018.8429
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Abstract

Osteoarthritis (OA) has a high prevalence in female patients and sex may be a key factor affecting the progression of OA. The aim of the present study was to identify genetic signatures in the synovial membranes of female patients with OA and to elucidate the potential associated molecular mechanisms. The gene expression profiles of the GSE55457 and GSE55584 datasets were obtained from the Gene Expression Omnibus database. Data of two synovial membranes from normal female individuals (GSM1337306 and GSM1337310) and two synovial membranes from female patients affected by OA (GSM1337327 and GSM1337330) were obtained from the dataset GSE55457, and those of three synovial membranes from female patients affected by OA (GSM1339628, GSM1339629 and GSM1339632) were obtained from the dataset GSE55584. Differentially expressed genes (DEGs) were identified by using Morpheus software. Protein‑protein interaction (PPI) networks of the DEGs were constructed by using Cytoscape software. Subsequently, Gene Ontology (GO) function and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses of the top module of the PPI network were performed by using ClueGo. A total of 377 DEGs were identified in the synovial membranes of OA patients compared with those of normal individuals, including 164 upregulated and 213 downregulated genes. The top 10 hub genes were ubiquitin (UB)C, ribosomal protein (RP) L23A, mammalian target of rapamycin, heat shock protein 90 α family class A member 1, RPS28, RPL37A, RPS24, RPS4X, RPS18 and UBB. The results of the GO analysis indicated that the DEGs included in the top module of the PPI were mainly enriched in the terms ‘nuclear‑transcribed mRNA catabolic process’, ‘nonsense mediated decay’, and ‘cytoplasmic translation and ribosomal small subunit biogenesis’. KEGG pathway analysis indicated that the DEGs included in the top one module were mainly enriched in the ‘ribosome’ pathway. The present study provides a systematic, molecular‑level understanding of the degeneration of the synovial membrane in the progression of OA in female patients. The hub genes and molecules associated with the synovial membrane may be used as biomarkers and therapeutic targets for the treatment of OA in female patients with OA.
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1 

Liu W, He J, Lin R, Liang J and Luo Q: Differential proteomics of the synovial membrane between bilateral and unilateral knee osteoarthritis in surgeryinduced rabbit models. Mol Med Rep. 14:2243–2249. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Chen S, Fang XQ, Zhang JF, Ma Y, Tang XZ, Zhou ZJ, Wang JY, Qin A and Fan SW: Lycorine protects cartilage through suppressing the expression of matrix metalloprotenases in rat chondrocytes and in a mouse osteoarthritis model. Mol Med Rep. 14:3389–3396. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Helmick CG, Felson DT, Lawrence RC, Gabriel S, Hirsch R, Kwoh CK, Liang MH, Kremers HM, Mayes MD, Merkel PA, et al: Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part I. Arthritis Rheum. 58:15–25. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Thomas E, Peat G and Croft P: Defining and mapping the person with osteoarthritis for population studies and public health. Rheumatology (Oxford). 53:338–345. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Kubosch EJ, Lang GM, Fürst D, Kubosch DC, Izadpanah K, Rolauffs B, Südkamp NP and Schmal H: The potential for synovium-derived stem cells in cartilage repair. Curr Stem Cell Res Ther. Oct 2–2017.(Epub ahead of print). PubMed/NCBI

6 

Mathiessen A and Conaghan PG: Synovitis in osteoarthritis: Current understanding with therapeutic implications. Arthritis Res Ther. 19:182017. View Article : Google Scholar : PubMed/NCBI

7 

Prieto-Potin I, Largo R, Roman-Blas JA, Herrero-Beaumont G and Walsh DA: Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis. BMC Musculoskelet Disord. 16:2262015. View Article : Google Scholar : PubMed/NCBI

8 

Deligne C, Casulli S, Pigenet A, Bougault C, Campillo-Gimenez L, Nourissat G, Berenbaum F, Elbim C and Houard X: Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients. Osteoarthritis Cartilage. 23:1843–1852. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Kuca-Warnawin EH, Kurowska WJ, Radzikowska A, Massalska MA, Burakowski T, Kontny E, Słowińska I, Gasik R and Maśliński W: Different expression of chemokines in rheumatoid arthritis and osteoarthritis bone marrow. Reumatologia. 54:51–53. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Takano S, Uchida K, Miyagi M, Inoue G, Fujimaki H, Aikawa J, Iwase D, Minatani A, Iwabuchi K and Takaso M: Nerve growth factor regulation by TNF-α and IL-1β in synovial macrophages and fibroblasts in osteoarthritic mice. J Immunol Res. 2016:57063592016. View Article : Google Scholar : PubMed/NCBI

11 

Salaffi F, Ciapetti A and Carotti M: The sources of pain in osteoarthritis: A pathophysiological review. Reumatismo. 66:57–71. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Das DK, Ali T, Krampis K and Ogunwobi OO: Fibronectin and androgen receptor expression data in prostate cancer obtained from a RNA-sequencing bioinformatics analysis. Data Brief. 11:131–135. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Gong C, Sun S, Liu B, Wang J and Chen X: Identification of potential therapeutic target genes, key miRNAs and mechanisms in oral lichen planus by bioinformatics analysis. Arch Oral Biol. 78:122–128. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Ramos YF, Bos SD, Lakenberg N, Böhringer S, den Hollander WJ, Kloppenburg M, Slagboom PE and Meulenbelt I: Genes expressed in blood link osteoarthritis with apoptotic pathways. Ann Rheum Dis. 73:1844–1853. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Ungethuem U, Haeupl T, Witt H, Koczan D, Krenn V, Huber H, von Helversen TM, Drungowski M, Seyfert C, Zacher J, et al: Molecular signatures and new candidates to target the pathogenesis of rheumatoid arthritis. Physiol Genomics. 42A:1–282. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Blagojevic M, Jinks C, Jeffery A and Jordan KP: Risk factors for onset of osteoarthritis of the knee in older adults: A systematic review and meta-analysis. Osteoarthritis Cartilage. 18:24–33. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Kee CC: Osteoarthritis: Manageable scourge of aging. Nurs Clin North Am. 35:199–208. 2000.PubMed/NCBI

18 

Venet D, Detours V and Bersini H: A measure of the signal-to-noise ratio of microarray samples and studies using gene correlations. PLoS One. 7:e510132012. View Article : Google Scholar : PubMed/NCBI

19 

Bindea G, Mlecnik B, Hackl H, Charoentong P, Tosolini M, Kirilovsky A, Fridman WH, Pagès F, Trajanoski Z and Galon J: ClueGO: A Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics. 25:1091–1032. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Oh C, Park S, Lee EK and Yoo YJ: Downregulation of ubiquitin level via knockdown of polyubiquitin gene Ubb as potential cancer therapeutic intervention. Sci Rep. 3:26232013. View Article : Google Scholar : PubMed/NCBI

21 

Tian Y, Ding W, Wang Y, Ji T, Sun S, Mo Q, Chen P, Fang Y, Liu J, Wang B, et al: Correction: Ubiquitin B in cervical cancer: Critical for the maintenance of cancer stem-like cell characters. PLoS One. 11:e01528132016. View Article : Google Scholar : PubMed/NCBI

22 

Zhang Y, Vasheghani F, Li YH, Blati M, Simeone K, Fahmi H, Lussier B, Roughley P, Lagares D, Pelletier JP, et al: Cartilage-specific deletion of mTOR upregulates autophagy and protects mice from osteoarthritis. Ann Rheum Dis. 74:1432–1440. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Ribeiro M, López de Figueroa P, Nogueira-Recalde U, Centeno A, Mendes AF, Blanco FJ and Caramés B: Diabetes-accelerated experimental osteoarthritis is prevented by autophagy activation. Osteoarthritis Cartilage. 24:2116–2125. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Ito Y, Hashimoto M, Hirota K, Ohkura N, Morikawa H, Nishikawa H, Tanaka A, Furu M, Ito H, Fujii T, et al: Detection of T cell responses to a ubiquitous cellular protein in autoimmune disease. Science. 346:363–368. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Tang YP and Wade J: Sex- and age-related differences in ribosomal proteins L17 and L37, as well as androgen receptor protein, in the song control system of zebra finches. Neuroscience. 171:1131–1140. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Wang Y, Sui J, Li X, Cao F, He J, Yang B, Zhu X, Sun Y and Pu YD: RPS24 knockdown inhibits colorectal cancer cell migration and proliferation in vitro. Gene. 571:286–291. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Badhai J, Fröjmark ASJ, Davey E, Schuster J and Dahl N: Ribosomal protein S19 and S24 insufficiency cause distinct cell cycle defects in Diamond-Blackfan anemia. Biochim Biophys Acta. 1792:1036–1042. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Anthony RA and Liebman SW: Alterations in ribosomal protein RPS28 can diversely affect translational accuracy in Saccharomyces cerevisiae. Genetics. 140:1247–1258. 1995.PubMed/NCBI

29 

Doherty L, Sheen MR, Vlachos A, Choesmel V, O'Donohue MF, Clinton C, Schneider HE, Sieff CA, Newburger PE, Ball SE, et al: Ribosomal protein genes RPS10 and RPS26 are commonly mutated in Diamond-Blackfan anemia. Am J Hum Genet. 86:222–228. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Gazda HT, Preti M, Sheen MR, O'Donohue MF, Vlachos A, Davies SM, Kattamis A, Doherty L, Landowski M, Buros C, et al: Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia. Hum Mutat. 33:1037–1044. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Gripp KW, Curry C, Olney AH, Sandoval C, Fisher J, Chong JX; UW Center for Mendelian Genomics, ; Pilchman L, Sahraoui R, Stabley DL and Sol-Church K: Diamond-Blackfan anemia with mandibulofacial dystostosis is heterogeneous, including the novel DBA genes TSR2 and RPS28. Am J Med Genet A. 164A:1–2249. 2014.PubMed/NCBI

32 

Shen H, Zhu H, Song M, Tian Y, Huang Y, Zheng H, Cao R, Lin J, Bi Z and Zhong W: A selenosemicarbazone complex with copper efficiently down-regulates the 90-kDa heat shock protein HSP90AA1 and its client proteins in cancer cells. BMC Cancer. 14:6292014. View Article : Google Scholar : PubMed/NCBI

33 

Hentze MW and Kulozik AE: A perfect message: RNA surveillance and nonsense-mediated decay. Cell. 96:307–310. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mi B, Liu G, Zhou W, Lv H, Liu Y and Liu J: Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis. Mol Med Rep 17: 4467-4473, 2018.
APA
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., & Liu, J. (2018). Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis. Molecular Medicine Reports, 17, 4467-4473. https://doi.org/10.3892/mmr.2018.8429
MLA
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., Liu, J."Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis". Molecular Medicine Reports 17.3 (2018): 4467-4473.
Chicago
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., Liu, J."Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis". Molecular Medicine Reports 17, no. 3 (2018): 4467-4473. https://doi.org/10.3892/mmr.2018.8429
Copy and paste a formatted citation
x
Spandidos Publications style
Mi B, Liu G, Zhou W, Lv H, Liu Y and Liu J: Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis. Mol Med Rep 17: 4467-4473, 2018.
APA
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., & Liu, J. (2018). Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis. Molecular Medicine Reports, 17, 4467-4473. https://doi.org/10.3892/mmr.2018.8429
MLA
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., Liu, J."Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis". Molecular Medicine Reports 17.3 (2018): 4467-4473.
Chicago
Mi, B., Liu, G., Zhou, W., Lv, H., Liu, Y., Liu, J."Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis". Molecular Medicine Reports 17, no. 3 (2018): 4467-4473. https://doi.org/10.3892/mmr.2018.8429
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