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Article Open Access

Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation

  • Authors:
    • Yongming Xu
    • Shujuan Xu
    • Zhi Gao
    • Liang Xiao
    • Fei Xiao
    • Hongguang Xu
    • Xiaoling Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Surgery, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Orthopedic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4415-4421
    |
    Published online on: January 16, 2018
       https://doi.org/10.3892/mmr.2018.8435
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Abstract

The aim of this study was to explore the degree of degeneration of endplate chondrocytes in different tension regions induced by intermittent cyclic mechanical tension (ICMT) in vitro. Rat endplate chondrocytes were harvested and treated with 10% ICMT for 8 h/day with a frequency of 0.5 Hz. A cartilage degeneration model was induced using an FX‑5000T cell strain‑loading system. The experiment was divided into the central region and the peripheral region, according to the contact area between the loading post and the six‑well flexible silicone rubber BioFlex plates. Toluidine blue and phalloidin staining were used to observe the morphological changes of cells following mechanical stimulation. Apoptosis was detected by flow cytometry and the mRNA and protein expression levels of collagen type II α1, aggrecan, SRY‑box 9 and matrix metalloproteinase 13 were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting, respectively. Endplate chondrocytes exhibited degenerative alterations under mechanical conditions of 10% ICMT and 0.5 Hz at 8 h/day. Toluidine blue and phalloidin staining demonstrated that the cells in the peripheral region were more slender compared with cells in the central region, but RT‑qPCR and western blotting results demonstrated that the degree of cell degeneration between the two groups was not statistically differences. So that cell morphological alteration does not imply that cells have undergone degeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Xu S, Gao Z, Xiao L, Xiao F, Xu H and Zhang X: Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation. Mol Med Rep 17: 4415-4421, 2018.
APA
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., & Zhang, X. (2018). Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation. Molecular Medicine Reports, 17, 4415-4421. https://doi.org/10.3892/mmr.2018.8435
MLA
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., Zhang, X."Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation". Molecular Medicine Reports 17.3 (2018): 4415-4421.
Chicago
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., Zhang, X."Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation". Molecular Medicine Reports 17, no. 3 (2018): 4415-4421. https://doi.org/10.3892/mmr.2018.8435
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Xu S, Gao Z, Xiao L, Xiao F, Xu H and Zhang X: Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation. Mol Med Rep 17: 4415-4421, 2018.
APA
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., & Zhang, X. (2018). Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation. Molecular Medicine Reports, 17, 4415-4421. https://doi.org/10.3892/mmr.2018.8435
MLA
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., Zhang, X."Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation". Molecular Medicine Reports 17.3 (2018): 4415-4421.
Chicago
Xu, Y., Xu, S., Gao, Z., Xiao, L., Xiao, F., Xu, H., Zhang, X."Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation". Molecular Medicine Reports 17, no. 3 (2018): 4415-4421. https://doi.org/10.3892/mmr.2018.8435
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