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Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine

  • Authors:
    • Yongmei Yang
    • Qingquan Chen
    • Sujie Jia
    • Limei He
    • Aiping Wang
    • Dai Li
    • Yuanjian Li
    • Xiaohui Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078, P.R. China, Department of Pharmacy, The Third Xiangya Hospitals, Central South University, Changsha, Hunan 410013, P.R. China, Department of Anatomy, School of Medicine, University of South China, Hengyang, Hunan 421001, P.R. China, National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5168-5174
    |
    Published online on: January 25, 2018
       https://doi.org/10.3892/mmr.2018.8494
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Abstract

The traditional Chinese herb Wu-Chu-Yu has been used to treat hypertension for hundreds of years. A previous study indicated that rutaecarpine was the effective component of Wu‑Chu‑Yu, which lowered blood pressure by elevating the expression level of calcitonin gene‑related peptide (CGRP). The present study was performed to investigate the role of transient receptor potential cation channel subfamily V member 1 (TRPV1) in CGRP expression and release induced by rutaecarpine. Dorsal root ganglia (DRG) obtained from Sprague‑Dawley rats were cultured to analyze the mRNA expression and release of CGRP. Calcium influx, as an indicator of TRPV1 activation, was measured in 293 cells with stable overexpression of TRPV1. The results demonstrated that the amount of CGRP in the cell culture supernatant and the mRNA expression of CGRPα and CGRPβ in DRG was upregulated by rutaecarpine in a concentration‑dependent manner, and was inhibited by the TRPV1 receptor antagonist capsazepine. In addition, intracellular Ca2+ levels were increased by Rut in the aforementioned 293 cell line, indicating the activation of TRPV1 by Rut. Therefore, it was concluded that TRPV1 was involved in the expression and release of CGRP stimulated by rutaecarpine, which provided novel mechanistic understanding of the treatment of hypertension using the Chinese herb Wu-Chu-Yu.
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1 

Li YJ and Peng J: The cardioprotection of calcitonin gene-related peptide-mediated preconditioning. Eur J Pharmacol. 442:173–177. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Vaishnava P and Wang DH: Capsaicin sensitive-sensory nerves and blood pressure regulation. Curr Med Chem Cardiovasc Hematol Agents. 1:177–188. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Portaluppi F, Trasforini G, Margutti A, Vergnani L, Ambrosio MR, Rossi R, Bagni B, Pansini R and Degli Uberti EC: Circadian rhythm of calcitonin gene-related peptide in uncomplicated essential hypertension. J Hypertens. 10:1227–1234. 1992. View Article : Google Scholar : PubMed/NCBI

4 

Deng PY, Ye F, Zhu HQ, Cai WJ, Deng HW and Li YJ: An increase in the synthesis and release of calcitonin gene-related peptide in two-kidney, one-clip hypertensive rats. Regul Pept. 114:175–182. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Freeland K, Liu YZ and Latchman DS: Distinct signalling pathways mediate the cAMP response element (CRE)-dependent activation of the calcitonin gene-related peptide gene promoter by cAMP and nerve growth factor. Biochem J. 345:233–238. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Garry MG, Walton LP and Davis MA: Capsaicin-evoked release of immunoreactive calcitonin gene-related peptide from the spinal cord is mediated by nitric oxide but not by cyclic GMP. Brain Res. 861:208–219. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Tang Y, Han C, Fiscus RR and Wang X: Increase of calcitonin gene-related peptide (CGRP) release and mRNA levels in endotoxic rats. Shock. 7:225–229. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Bracci-Laudiero L, Aloe L, Caroleo MC, Buanne P, Costa N, Starace G and Lundeberg T: Endogenous NGF regulates CGRP expression in human monocytes, and affects HLA-DR and CD86 expression and IL-10 production. Blood. 106:3507–3514. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Wang DH: The vanilloid receptor and hypertension. Acta Pharmacol Sin. 26:286–294. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Xu DC: One hundred species of recorded from Sheng Nong's herbal classic. Shanghai People's Publishing House; pp. 401736

11 

Xu S, Peng J, Li Y, He L, Chen F, Zhang J and Ding J: Pharmacokinetic comparisons of rutaecarpine and evodiamine after oral administration of Wu-Chu-Yu extracts with different purities to rats. J Ethnopharmacol. 139:395–400. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Chen Z, Hu G, Li D, Chen J, Li Y, Zhou H and Xie Y: Synthesis and vasodilator effects of rutaecarpine analogues which might be involved transient receptor potential vanilloid subfamily, member 1 (TRPV1). Bioorg Med Chem. 17:2351–2359. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Li D, Peng J, Xin HY, Luo D, Zhang YS, Zhou Z, Jiang DJ, Deng HW and Li YJ: Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats. Peptides. 29:1781–1788. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Deng PY, Ye F, Cai WJ, Tan GS, Hu CP, Deng HW and Li YJ: Stimulation of calcitonin gene-related peptide synthesis and release: Mechanisms for a novel antihypertensive drug, rutaecarpine. J Hypertens. 22:1819–1829. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Li D, Zhang XJ, Chen L, Yang Z, Deng HW, Peng J and Li YJ: Calcitonin gene-related peptide mediates the cardioprotective effects of rutaecarpine against ischaemia-reperfusion injury in spontaneously hypertensive rats. Clin Exp Pharmacol Physiol. 36:662–667. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Yu J, Tan GS, Deng PY, Xu KP, Hu CP and Li YJ: Involvement of CGRP in the inhibitory effect of rutaecarpine on vasoconstriction induced by anaphylaxis in guinea pig. Regul Pept. 125:93–97. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Luo XJ, Li NS, Zhang YS, Liu B, Yang ZC, Li YJ, Dong XR and Peng J: Vanillyl nonanoate protects rat gastric mucosa from ethanol-induced injury through a mechanism involving calcitonin gene-related peptide. Eur J Pharmacol. 666:211–217. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Demirbilek S, Ersoy MO, Demirbilek S, Karaman A, Gürbüz N, Bayraktar N and Bayraktar M: Small-dose capsaicin reduces systemic inflammatory responses in septic rats. Anesth Analg. 99:1501–1507. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Bergman J and Bergman S: Studies of rutaecarpine and related quinazolinocarboline alkaloids. J Org Chem. 50:1246–1255. 1985. View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Banerjee S, Evanson J, Harris E, Lowe SL, Thomasson KA and Porter JE: Identification of specific calcitonin-like receptor residues important for calcitonin gene-related peptide high affinity binding. BMC Pharmacol. 6:92006. View Article : Google Scholar : PubMed/NCBI

22 

Li J and Wang DH: Development of angiotensin II-induced hypertension: Role of CGRP and its receptor. J Hypertens. 23:113–118. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Li XW, Hu CP, Wu WH, Zhang WF, Zou XZ and Li YJ: Inhibitory effect of calcitonin gene-related peptide on hypoxia-induced rat pulmonary artery smooth muscle cells proliferation: Role of ERK1/2 and p27. Eur J Pharmacol. 679:117–126. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Shichiri M, Kato H, Doi M, Marumo F and Hirata Y: Induction of max by adrenomedullin and calcitonin gene-related peptide antagonizes endothelial apoptosis. Mol Endocrinol. 13:1353–1363. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Sánchez MG, Ruiz-Llorente L, Sánchez AM and Diaz-Laviada I: Activation of phosphoinositide 3-kinase/PKB pathway by CB(1) and CB(2) cannabinoid receptors expressed in prostate PC-3 cells. Involvement in Raf-1 stimulation and NGF induction. Cell Signal. 15:851–859. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Vellani V, Zachrisson O and McNaughton PA: Functional bradykinin B1 receptors are expressed in nociceptive neurones and are upregulated by the neurotrophin GDNF. J Physiol. 560:391–401. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Hou L and Wang X: PKC and PKA, but not PKG mediate LPS-induced CGRP release and [Ca(2+)](i) elevation in DRG neurons of neonatal rats. J Neurosci Res. 66:592–600. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Haug T and Storm JF: Protein kinase A mediates the modulation of the slow Ca(2+)-dependent K(+) current, I(sAHP), by the neuropeptides CRF, VIP, and CGRP in hippocampal pyramidal neurons. J Neurophysiol. 83:2071–2079. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Caterina MJ, Rosen TA, Tominaga M, Brake AJ and Julius D: A capsaicin-receptor homologue with a high threshold for noxious heat. Nature. 398:436–441. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Tominaga M, Caterina MJ, Malmberg AB, Rosen TA, Gilbert H, Skinner K, Raumann BE, Basbaum AI and Julius D: The cloned capsaicin receptor integrates multiple pain-producing stimuli. Neuron. 21:531–543. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Caterina MJ and Julius D: The vanilloid receptor: A molecular gateway to the pain pathway. Annu Rev Neurosci. 24:487–517. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Hu CP, Xiao L, Deng HW and Li YJ: The depressor and vasodilator effects of rutaecarpine are mediated by calcitonin gene-related peptide. Planta Med. 69:125–129. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Wang GJ, Wu XC, Chen CF, Lin LC, Huang YT, Shan J and Pang PK: Vasorelaxing action of rutaecarpine: Effects of rutaecarpine on calcium channel activities in vascular endothelial and smooth muscle cells. J Pharmacol Exp Ther. 289:1237–1244. 1999.PubMed/NCBI

34 

Smith PJ and McQueen DS: Anandamide induces cardiovascular and respiratory reflexes via vasosensory nerves in the anaesthetized rat. Br J Pharmacol. 134:655–663. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Chen Q, Jia S, He L, Wang A, Li D, Li Y and Li X: Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine. Mol Med Rep 17: 5168-5174, 2018.
APA
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D. ... Li, X. (2018). Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine. Molecular Medicine Reports, 17, 5168-5174. https://doi.org/10.3892/mmr.2018.8494
MLA
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D., Li, Y., Li, X."Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine". Molecular Medicine Reports 17.4 (2018): 5168-5174.
Chicago
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D., Li, Y., Li, X."Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine". Molecular Medicine Reports 17, no. 4 (2018): 5168-5174. https://doi.org/10.3892/mmr.2018.8494
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Chen Q, Jia S, He L, Wang A, Li D, Li Y and Li X: Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine. Mol Med Rep 17: 5168-5174, 2018.
APA
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D. ... Li, X. (2018). Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine. Molecular Medicine Reports, 17, 5168-5174. https://doi.org/10.3892/mmr.2018.8494
MLA
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D., Li, Y., Li, X."Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine". Molecular Medicine Reports 17.4 (2018): 5168-5174.
Chicago
Yang, Y., Chen, Q., Jia, S., He, L., Wang, A., Li, D., Li, Y., Li, X."Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine". Molecular Medicine Reports 17, no. 4 (2018): 5168-5174. https://doi.org/10.3892/mmr.2018.8494
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