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Article

Effect of idebenone on bone marrow mesenchymal stem cells in vitro

  • Authors:
    • Jiahui Zhang
    • Jun Zhang
    • Tao Li
    • Nannan Zhang
    • Shengnan Tang
    • Zehua Tao
    • Xiaohe Zhou
    • Xiaochun Sun
    • Huabiao Chen
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Clinical Laboratory, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
  • Pages: 5376-5383
    |
    Published online on: January 26, 2018
       https://doi.org/10.3892/mmr.2018.8506
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Abstract

In recent years, stem cell research has continued to benefit from its crossover with chemistry, particularly the investigation of small molecular drugs modulating specific targets to regulate stem cell fate. Idebenone (IDB) is a yellow crystalline powder that is used in the treatment of chronic cerebrovascular diseases. The objective of the present study was to examine whether IDB had an influence on bone marrow‑derived mesenchymal stem cells (BMSCs) extracted from the bone marrow of Sprague‑Dawley rats. The effects of IDB on cell proliferation, cell cloning and migration were investigated. Cell cycle, apoptosis, DAPI nuclear staining and senescence‑associated β‑galactosidase (SA‑β‑gal) staining were also examined. The results revealed that IDB at suitable concentrations enhanced cell cloning capacity, promoted the proliferation of BMSCs, delayed cellular senescence, and inhibited cell apoptosis and migration. Western blot analysis indicated that IDB increased the expression of B‑cell lymphoma 2 (Bcl‑2), signal transducer and activator of transcription‑3, Nanog, octamer‑binding transcription factor 4, E‑cadherin, proliferating cell nuclear antigen, cyclinD1 and cyclinD3, and decreased the expression of Bcl‑2‑associated X protein, cleaved caspase‑3, N‑cadherin, vimentin and α‑smooth muscle actin. In conclusion, these experiments confirmed that IDB in low doses had no toxic effect and may exert protective effects on BMSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Zhang J, Li T, Zhang N, Tang S, Tao Z, Zhou X, Sun X and Chen H: Effect of idebenone on bone marrow mesenchymal stem cells in vitro. Mol Med Rep 17: 5376-5383, 2018.
APA
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z. ... Chen, H. (2018). Effect of idebenone on bone marrow mesenchymal stem cells in vitro. Molecular Medicine Reports, 17, 5376-5383. https://doi.org/10.3892/mmr.2018.8506
MLA
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z., Zhou, X., Sun, X., Chen, H."Effect of idebenone on bone marrow mesenchymal stem cells in vitro". Molecular Medicine Reports 17.4 (2018): 5376-5383.
Chicago
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z., Zhou, X., Sun, X., Chen, H."Effect of idebenone on bone marrow mesenchymal stem cells in vitro". Molecular Medicine Reports 17, no. 4 (2018): 5376-5383. https://doi.org/10.3892/mmr.2018.8506
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Zhang J, Li T, Zhang N, Tang S, Tao Z, Zhou X, Sun X and Chen H: Effect of idebenone on bone marrow mesenchymal stem cells in vitro. Mol Med Rep 17: 5376-5383, 2018.
APA
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z. ... Chen, H. (2018). Effect of idebenone on bone marrow mesenchymal stem cells in vitro. Molecular Medicine Reports, 17, 5376-5383. https://doi.org/10.3892/mmr.2018.8506
MLA
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z., Zhou, X., Sun, X., Chen, H."Effect of idebenone on bone marrow mesenchymal stem cells in vitro". Molecular Medicine Reports 17.4 (2018): 5376-5383.
Chicago
Zhang, J., Zhang, J., Li, T., Zhang, N., Tang, S., Tao, Z., Zhou, X., Sun, X., Chen, H."Effect of idebenone on bone marrow mesenchymal stem cells in vitro". Molecular Medicine Reports 17, no. 4 (2018): 5376-5383. https://doi.org/10.3892/mmr.2018.8506
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