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Article Open Access

Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma

  • Authors:
    • Yu‑Yuan Qian
    • Zhi‑Su Liu
    • Zidong Zhang
    • Anait S. Levenson
    • Kun Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Surgery, School of Medicine, Saint Louis University, St. Louis, MO 63103, USA, Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39762, USA
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5193-5201
    |
    Published online on: January 29, 2018
       https://doi.org/10.3892/mmr.2018.8515
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Abstract

Pterostilbene (Pter) is reported to exhibit an anticancer effect in hepatocellular carcinoma (HCC). In order to explore the anticancer mechanism in HCC cells, the present study aimed to investigate whether pterostilbene (Pter) may increase phosphatase and tensin homolog (PTEN) expression through targeted downregulation of microRNA (miRNA/miR)-19a in hepatocellular carcinoma (HCC). The proliferation, apoptosis and cell cycle was analyzed in the SMMC‑7721 HCC cell line by MTT assays and flow cytometry methods. Cells were divided into five treatment groups: Pter treatment, miR‑19a inhibitor transfection, Pter + miR‑19a inhibitor, negative control transfection and blank control. The expression of miR‑19a and PTEN was detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis following treatment. Furthermore, a luciferase reporter gene assay was performed to determine whether the PTEN gene was a direct target of miR‑19a. The results demonstrated that Pter treatment or miR‑19a inhibitor transfection downregulated miR‑19a and induced PTEN/Akt pathway regulation, which led to proliferation inhibition, cell cycle arrest in the S phase, increased apoptosis and reduced cell invasion. These results indicated that Pter may increase PTEN expression through the direct downregulation of miR‑19a in HCC. Therefore, miR‑19a may have potential as a novel molecular marker for HCC and Pter may be a promising clinical target with the potential to be developed as a HCC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Qian YY, Liu ZS, Zhang Z, Levenson AS and Li K: Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma. Mol Med Rep 17: 5193-5201, 2018.
APA
Qian, Y., Liu, Z., Zhang, Z., Levenson, A.S., & Li, K. (2018). Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma. Molecular Medicine Reports, 17, 5193-5201. https://doi.org/10.3892/mmr.2018.8515
MLA
Qian, Y., Liu, Z., Zhang, Z., Levenson, A. S., Li, K."Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma". Molecular Medicine Reports 17.4 (2018): 5193-5201.
Chicago
Qian, Y., Liu, Z., Zhang, Z., Levenson, A. S., Li, K."Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma". Molecular Medicine Reports 17, no. 4 (2018): 5193-5201. https://doi.org/10.3892/mmr.2018.8515
Copy and paste a formatted citation
x
Spandidos Publications style
Qian YY, Liu ZS, Zhang Z, Levenson AS and Li K: Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma. Mol Med Rep 17: 5193-5201, 2018.
APA
Qian, Y., Liu, Z., Zhang, Z., Levenson, A.S., & Li, K. (2018). Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma. Molecular Medicine Reports, 17, 5193-5201. https://doi.org/10.3892/mmr.2018.8515
MLA
Qian, Y., Liu, Z., Zhang, Z., Levenson, A. S., Li, K."Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma". Molecular Medicine Reports 17.4 (2018): 5193-5201.
Chicago
Qian, Y., Liu, Z., Zhang, Z., Levenson, A. S., Li, K."Pterostilbene increases PTEN expression through the targeted downregulation of microRNA-19a in hepatocellular carcinoma". Molecular Medicine Reports 17, no. 4 (2018): 5193-5201. https://doi.org/10.3892/mmr.2018.8515
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