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Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes

  • Authors:
    • Chencheng Feng
    • Minghan Liu
    • Xin Fan
    • Minghui Yang
    • Huan Liu
    • Yue Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5238-5246
    |
    Published online on: January 30, 2018
       https://doi.org/10.3892/mmr.2018.8517
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Abstract

Previous studies have identified the association between cartilage endplate (CEP) degeneration and abnormal mechanical loading. Several studies have reported that intermittent cyclic mechanical tension (ICMT) regulates CEP degeneration via various biological processes and signaling pathways. However, the functions of microRNAs in regulating the cellular responses of CEP chondrocytes to ICMT remain to be elucidated. The current study determined the differentially expressed microRNAs in human CEP chondrocytes exposed to ICMT using microarray analysis. A total 21 significantly upregulated and 62 downregulated miRNAs were identified compared with the control. The findings were subsequently partially validated by reverse transcription‑quantitative polymerase chain reaction. Potential target genes of the significantly differentially expressed miRNAs were predicted using bioinformatics analysis and were used for Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The present study revealed that the significantly differentially expressed microRNAs were involved in various signaling pathways and biological processes that are crucial to regulating the responses of CEP chondrocytes to ICMT. The current study provided a global view of microRNA expression in CEP chondrocytes under mechanical stimulation, suggesting that microRNAs are important for regulating the mechanical response of CEP chondrocytes. Additionally, it provided a novel insight into the association between mechanical stress and the establishment and progression of intervertebral disc degeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Feng C, Liu M, Fan X, Yang M, Liu H and Zhou Y: Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes. Mol Med Rep 17: 5238-5246, 2018.
APA
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., & Zhou, Y. (2018). Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes. Molecular Medicine Reports, 17, 5238-5246. https://doi.org/10.3892/mmr.2018.8517
MLA
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., Zhou, Y."Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes". Molecular Medicine Reports 17.4 (2018): 5238-5246.
Chicago
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., Zhou, Y."Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes". Molecular Medicine Reports 17, no. 4 (2018): 5238-5246. https://doi.org/10.3892/mmr.2018.8517
Copy and paste a formatted citation
x
Spandidos Publications style
Feng C, Liu M, Fan X, Yang M, Liu H and Zhou Y: Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes. Mol Med Rep 17: 5238-5246, 2018.
APA
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., & Zhou, Y. (2018). Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes. Molecular Medicine Reports, 17, 5238-5246. https://doi.org/10.3892/mmr.2018.8517
MLA
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., Zhou, Y."Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes". Molecular Medicine Reports 17.4 (2018): 5238-5246.
Chicago
Feng, C., Liu, M., Fan, X., Yang, M., Liu, H., Zhou, Y."Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes". Molecular Medicine Reports 17, no. 4 (2018): 5238-5246. https://doi.org/10.3892/mmr.2018.8517
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