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Article

Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

  • Authors:
    • Wei Zhang
    • Shanshan Zhou
    • Yuwei Gao
    • Hongquan Song
    • Xiaohui Jiao
    • Xiaotong Wang
    • Yong Li
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Neurology, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 5396-5401
    |
    Published online on: January 30, 2018
       https://doi.org/10.3892/mmr.2018.8521
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Abstract

Maternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate formation in mice. This TCDD treatment, which may be considered an environmental factor in cleft palate formation, is associated with alterations in DNA methylation. However, the underlying molecular mechanisms of DNA methylation produced by TCDD in mouse embryos are poorly understood. DNA methyltransferases (DNMTs) and methyl‑CpG binding domain proteins (MBDs) are thought to be closely associated with the actions of DNA methylation. Therefore, the present study tested the hypothesis that this cleft palate inducing effect of TCDD will alter the expression levels of DNMTs and various MBDs in palate tissue of fetal mice. Pregnant C57BL/6J mice were treated with either TCDD (64 µg/kg) or corn oil (control) at embryonic day 10.5 (E10.5) and fetal palates were harvested for structural and molecular analyses at E13.5, E14.5, E15.5 and E17.5. Expression levels of DNMTs and MBDs were assayed using reverse transcription‑quantitative polymerase chain reaction and western blotting. The incidence of cleft palates in the TCDD group was 98.24%, whereas no cases of cleft palate were observed in the control group. Expression levels of DNMTs and MBDs were significantly increased in the TCDD group compared with the control. The results demonstrate clear alterations in DNMTs and MBDs, as induced by TCDD, and suggest that such alterations are important in cleft palate formation in fetal mice.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Zhou S, Gao Y, Song H, Jiao X, Wang X and Li Y: Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. Mol Med Rep 17: 5396-5401, 2018.
APA
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., & Li, Y. (2018). Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. Molecular Medicine Reports, 17, 5396-5401. https://doi.org/10.3892/mmr.2018.8521
MLA
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., Li, Y."Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice". Molecular Medicine Reports 17.4 (2018): 5396-5401.
Chicago
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., Li, Y."Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice". Molecular Medicine Reports 17, no. 4 (2018): 5396-5401. https://doi.org/10.3892/mmr.2018.8521
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Zhou S, Gao Y, Song H, Jiao X, Wang X and Li Y: Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. Mol Med Rep 17: 5396-5401, 2018.
APA
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., & Li, Y. (2018). Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. Molecular Medicine Reports, 17, 5396-5401. https://doi.org/10.3892/mmr.2018.8521
MLA
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., Li, Y."Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice". Molecular Medicine Reports 17.4 (2018): 5396-5401.
Chicago
Zhang, W., Zhou, S., Gao, Y., Song, H., Jiao, X., Wang, X., Li, Y."Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice". Molecular Medicine Reports 17, no. 4 (2018): 5396-5401. https://doi.org/10.3892/mmr.2018.8521
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