MicroRNA expression profiling in placenta and maternal plasma in early pregnancy loss

Hosseini,Mohammad  Kazem; Gunel,Tuba; Gumusoglu,Ece; Benian,Ali; Aydinli,Kilic

doi:10.3892/mmr.2018.8530

MicroRNA expression profiling in placenta and maternal plasma in early pregnancy loss

Authors:
- Mohammad Kazem Hosseini
- Tuba Gunel
- Ece Gumusoglu
- Ali Benian
- Kilic Aydinli
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Affiliations: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul 34134, Turkey, Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul 34098, Turkey, Medicus Health Center, Istanbul 34365, Turkey
Published online on: January 31, 2018 https://doi.org/10.3892/mmr.2018.8530
Pages: 4941-4952
Copyright: © Hosseini et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Early pregnancy loss (EPL), also termed early miscarriage, is determined as the unintentional expulsion of an embryo or fetus prior to the 12th week of gestation. EPL frequency is ~15% in pregnancies. Fetal development and growth is associate with placental function and vessel development; therefore, the placental genome would represent a useful miscarriage model for (epi)genetic and genomic studies. An important factor of placental development and function is epigenetic regulation of gene expression. microRNAs (miRNAs) are the primary epigenetic regulators which have an important role in placental development and function. In the present study, maternal plasma and villous tissue were collected from 16 EPL cases in 6th‑8th gestational weeks (GWs) and 8 abortions (control group) in 6th‑8th GWs. Detection of the differences in miRNA expression was performed using microarrays and dysregulated miRNAs were validated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). miRNA microarray findings revealed that four miRNAs, including hsa‑miRNA (miR)‑125a‑3p, hsa‑miR‑3663‑3p, hsa‑miR‑423‑5p and hsa‑miR‑575 were upregulated in tissue samples. In maternal plasma, two miRNAs (hsa‑let‑7c, hsa‑miR‑122) were upregulated and one miRNA (hsa‑miR‑135a) was downregulated. A total of 6 out of 7 dysregulated miRNAs were validated using RT‑qPCR. The target genes of these dysregulated miRNAs were detected using the GeneSpring database. The aim of the present study was to detect dysregulated miRNAs in maternal plasma and villous cells and identify the target genes of dysregulated miRNAs and their associated pathways. The target gene analyses have revealed that the affected genes are primarily associated with cell migration, proliferation, implantation, adhesion, angiogenesis and differentiation and all are involved with EPL pathogenesis. Therefore, the present study may contribute to the understanding of the molecular mechanisms which lead to EPL.

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