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Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

  • Authors:
    • Jiangang Liu
    • Zhongjie Liu
    • Xiaohui Hu
    • Yuan Zhang
    • Shiming Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Neurosurgery, Shiqianxian People's Hospital, Guizhou 555100, P.R. China, Department of Radiology, Suzhou Guangji Hospital, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5065-5073
    |
    Published online on: February 1, 2018
       https://doi.org/10.3892/mmr.2018.8536
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Abstract

During the development of postoperative vascular restenosis, the aberrant proliferation of vascular smooth muscle cells (VSMCs) is a critical event resulting in intimal hyperplasia. Inflammatory responses involving the activation of nuclear factor (NF)‑κB are among the major molecular processes underlying restenosis. The present study aimed to investigate the roles of NF‑κB in VSMC proliferation and restenosis following vascular anastomosis, as well as to evaluate the potential of synthetic E‑selectin to downregulate NF‑κB and thus inhibit vascular hyperplasia. A total of 72 adult male Sprague‑Dawley rats were randomly assigned to three groups: Control, operation and treatment groups. Rats in the operation and treatment groups received longitudinal incisions in the right carotid arteries, which were closed using interrupted sutures. Following vascular anastomosis, synthetic E‑selectin (10 mg/kg), or an equal volume of saline, was immediately injected into the right femoral vein of rats in the treatment and operation groups, respectively. Following surgery, the mRNA and protein expression levels of NF‑κB at the site of anastomosis, the levels of tumor necrosis factor‑α and interleukin‑6 in the serum, NF‑κB binding activity, and the presence of proliferating cell nuclear antigen (PCNA)‑positive cells were evaluated by western blotting, reverse transcription‑quantitative polymerase chain reaction, ELISA, electrophoretic mobility shift assay and immunofluorescence staining. The present results demonstrated that following treatment with synthetic E‑selectin, the levels of NF‑κB and the inflammatory response, as well as the presence of PCNA‑positive cells, were significantly reduced (P<0.01). In conclusion, the results of the present study suggested that synthetic E‑selectin may exert anti‑inflammatory and anti‑restenotic effects following vascular anastomosis in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Liu Z, Hu X, Zhang Y and Zhang S: Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats. Mol Med Rep 17: 5065-5073, 2018.
APA
Liu, J., Liu, Z., Hu, X., Zhang, Y., & Zhang, S. (2018). Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats. Molecular Medicine Reports, 17, 5065-5073. https://doi.org/10.3892/mmr.2018.8536
MLA
Liu, J., Liu, Z., Hu, X., Zhang, Y., Zhang, S."Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats". Molecular Medicine Reports 17.4 (2018): 5065-5073.
Chicago
Liu, J., Liu, Z., Hu, X., Zhang, Y., Zhang, S."Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats". Molecular Medicine Reports 17, no. 4 (2018): 5065-5073. https://doi.org/10.3892/mmr.2018.8536
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Liu Z, Hu X, Zhang Y and Zhang S: Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats. Mol Med Rep 17: 5065-5073, 2018.
APA
Liu, J., Liu, Z., Hu, X., Zhang, Y., & Zhang, S. (2018). Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats. Molecular Medicine Reports, 17, 5065-5073. https://doi.org/10.3892/mmr.2018.8536
MLA
Liu, J., Liu, Z., Hu, X., Zhang, Y., Zhang, S."Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats". Molecular Medicine Reports 17.4 (2018): 5065-5073.
Chicago
Liu, J., Liu, Z., Hu, X., Zhang, Y., Zhang, S."Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats". Molecular Medicine Reports 17, no. 4 (2018): 5065-5073. https://doi.org/10.3892/mmr.2018.8536
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