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Article

Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro

  • Authors:
    • Fan Wu
    • Peng Tian
    • Yun Ma
    • Jingyi Wang
    • Huashuang Ou
    • Hua Zou
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China
  • Pages: 5384-5389
    |
    Published online on: February 1, 2018
       https://doi.org/10.3892/mmr.2018.8540
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Abstract

The present study aimed to evaluate the pro-apoptotic effects of bleomycin A5 on nasal polyp‑derived fibroblasts (NPDFs) and the underlying molecular mechanisms. Nasal polyp tissue was acquired from 10 patients during surgery and NPDFs were isolated from surgical tissues. Fibroblasts were identified using immunohistochemistry. Bleomycin A5 was used to treat NPDFs along a concentration gradient. Cell viability was evaluated using a Cell Counting Kit‑8 assay. A flow cytometric Annexin V‑fluorescein isothiocyanate/propidium iodide assay was used to determine the percentage of apoptotic NPDFs. The mRNA expression levels of apoptotic genes were determined by reverse transcription‑quantitative polymerase chain reaction and levels of proteins associated with apoptosis were determined by western blotting. The results indicated that bleomycin A5 was able to induce apoptosis in NPDFs in a dose‑dependent manner. NPDFs treated with bleomycin A5 were identified to contain significantly high amounts of the active forms of caspase‑3 and showed considerable cleavage of poly(ADP‑ribose) polymerase. The mRNA and protein expression levels of the pro‑apoptotic molecule Bcl‑2‑associated X protein were significantly higher in treated NPDFs than in untreated NPDFs. In contrast, the mRNA and protein expression of the anti‑apoptotic molecule B‑cell lymphoma 2 (Bcl‑2) was significantly lower in treated NPDFs. These results indicated that bleomycin A5 could induce apoptosis in primary NPDFs through activation of the Bcl‑2 family and caspase cascades in a time-, and concentration-dependent manner.
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1 

Thomas M, Yawn BP, Price D, Lund V, Mullol J and Fokkens W; European Position Paper on Rhinosinusitis and Nasal Polyps Group, : EPOS primary care guidelines: European position paper on the primary care diagnosis and management of rhinosinusitis and nasal polyps 2007-a summary. Prim Care Respir J. 17:79–89. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Hedman J, Kaprio J, Poussa T and Nieminen MM: Prevalence of asthma, aspirin intolerance, nasal polyposis and chronic obstructive pulmonary disease in a population-based study. Int J Epidemiol. 28:717–722. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Wang QP, Escudier E, Roudot-Thoraval F, Abd-Al Samad I, Peynegre R and Coste A: Myofibroblast accumulation induced by transforming growth factor-beta is involved in the pathogenesis of nasal polyps. Laryngoscope. 107:926–931. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Park IH, Park SJ, Cho JS, Moon YM, Kim TH, Lee SH and Lee HM: Role of reactive oxygen species in transforming growth factor beta1-induced alpha smooth-muscle actin and collagen production in nasal polyp-derived fibroblasts. Int Arch Allergy Immunol. 159:278–286. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Park HH, Park IH, Cho JS, Lee YM and Lee HM: The effect of macrolides on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts. Am J Rhinol Allergy. 24:348–353. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Shin JM, Park JH, Park IH and Lee HM: Doxycycline inhibits TGF-β1-induced extracellular matrix production in nasal polyp-derived fibroblasts. Int Forum Allergy Rhinol. 6:256–263. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Ledon JA, Savas J, Franca K, Chacon A and Nouri K: Intralesional treatment for keloids and hypertrophic scars: A review. Dermatol Surg. 39:1745–1757. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Jones CD, Guiot L, Samy M, Gorman M and Tehrani H: The use of chemotherapeutics for the treatment of keloid scars. Dermatol Reports. 7:58802015. View Article : Google Scholar : PubMed/NCBI

9 

Hendricks T, Martens MF, Huyben CM and Wobbes T: Inhibition of basal and TGF beta-induced fibroblast collagen synthesis by antineoplastic agents. Implications for wound healing. Br J Cancer. 67:545–550. 1993. View Article : Google Scholar : PubMed/NCBI

10 

Yeowell HN, Marshall MK, Walker LC, Ha V and Pinnell SR: Regulation of lysyl oxidase mRNA in dermal fibroblasts from normal donors and patients with inherited connective tissue disorders. Arch Biochem Biophys. 308:299–305. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Wallach-Dayan SB, Izbicki G, Cohen PY, Gerstl-Golan R, Fine A and Breuer R: Bleomycin initiates apoptosis of lung epithelial cells by ROS but not by Fas/FasL pathway. Am J Physiol Lung Cell Mol Physiol. 290:L790–L796. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Wang R, Ibarra-Sunga O, Verlinski L, Pick R and Uhal BD: Abrogation of bleomycin-induced epithelial apoptosis and lung fibrosis by captopril or by a caspase inhibitor. Am J Physiol Lung Cell Mol Physiol. 279:L143–L151. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Zhang X, Zou J, Li B, Ren X and Shi J: Eosinophil apoptosis in nasal polyposis tissue after bleomycin A5 local injection. Lin Chuang Er Bi Yan Hou Ke Za Zhi. 18:279–281. 2004.(In Chinese). PubMed/NCBI

14 

Park IH, Um JY, Hong SM, Cho JS, Lee SH, Lee SH and Lee HM: Metformin reduces TGF-β1-induced extracellular matrix production in nasal polyp-derived fibroblasts. Otolaryngol Head Neck Surg. 150:148–153. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Sharifi S, Barar J, Hejazi MS and Samadi N: Roles of the Bcl-2/Bax ratio, caspase-8 and 9 in resistance of breast cancer cells to paclitaxel. Asian Pac J Cancer Prev. 15:8617–8622. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Tsukui T, Ueha S, Abe J, Hashimoto S, Shichino S, Shimaoka T, Shand FH, Arakawa Y, Oshima K, Hattori M, et al: Qualitative rather than quantitative changes are hallmarks of fibroblasts in bleomycin-induced pulmonary fibrosis. Am J Pathol. 183:758–773. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Wang XQ, Liu YK, Qing C and Lu SL: A review of the effectiveness of antimitotic drug injections for hypertrophic scars and keloids. Ann Plast Surg. 63:688–692. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Li X, Zhang H, Soledad-Conrad V, Zhuang J and Uhal BD: Bleomycin-induced apoptosis of alveolar epithelial cells requires angiotensin synthesis de novo. Am J Physiol Lung Cell Mol Physiol. 284:L501–L507. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Bleackley RC and Heibein JA: Enzymatic control of apoptosis. Nat Prod Rep. 18:431–440. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Budinger GR, Mutlu GM, Eisenbart J, Fuller AC, Bellmeyer AA, Baker CM, Wilson M, Ridge K, Barrett TA, Lee VY and Chandel NS: Proapoptotic Bid is required for pulmonary fibrosis. Proc Natl Acad Sci USA. 103:pp. 4604–4609. 2006; View Article : Google Scholar : PubMed/NCBI

21 

Kasper M and Barth K: Bleomycin and its role in inducing apoptosis and senescence in lung cells-modulating effects of caveolin-1. Curr Cancer Drug Targets. 9:341–353. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Chen YB, Aon MA, Hsu YT, Soane L, Teng X, McCaffery JM, Cheng WC, Qi B, Li H, Alavian KN, et al: Bcl-×L regulates mitochondrial energetics by stabilizing the inner membrane potential. J Cell Biol. 195:263–276. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Prenek L, Boldizsár F, Kugyelka R, Ugor E, Berta G, Németh P and Berki T: The regulation of the mitochondrial apoptotic pathway by glucocorticoid receptor in collaboration with Bcl-2 family proteins in developing T cells. Apoptosis. 22:239–253. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu F, Tian P, Ma Y, Wang J, Ou H and Zou H: Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro. Mol Med Rep 17: 5384-5389, 2018.
APA
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., & Zou, H. (2018). Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro. Molecular Medicine Reports, 17, 5384-5389. https://doi.org/10.3892/mmr.2018.8540
MLA
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., Zou, H."Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro". Molecular Medicine Reports 17.4 (2018): 5384-5389.
Chicago
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., Zou, H."Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro". Molecular Medicine Reports 17, no. 4 (2018): 5384-5389. https://doi.org/10.3892/mmr.2018.8540
Copy and paste a formatted citation
x
Spandidos Publications style
Wu F, Tian P, Ma Y, Wang J, Ou H and Zou H: Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro. Mol Med Rep 17: 5384-5389, 2018.
APA
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., & Zou, H. (2018). Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro. Molecular Medicine Reports, 17, 5384-5389. https://doi.org/10.3892/mmr.2018.8540
MLA
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., Zou, H."Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro". Molecular Medicine Reports 17.4 (2018): 5384-5389.
Chicago
Wu, F., Tian, P., Ma, Y., Wang, J., Ou, H., Zou, H."Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro". Molecular Medicine Reports 17, no. 4 (2018): 5384-5389. https://doi.org/10.3892/mmr.2018.8540
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