Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells

Yan,Yu; Qian,Hua; Jiang,Hongda; Yu,Haiyang; Sun,Liyao; Wei,Xi; Sun,Yunduan; Ge,Hongyan; Zhou,Haizhou; Li,Xiaoguang; Hashimoto,Takashi; Tang,Xianling; Liu,Ping

doi:10.3892/mmr.2018.8581

April-2018 Volume 17 Issue 4

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April-2018 Volume 17 Issue 4

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Article Open Access

Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells

Authors:
- Yu Yan
- Hua Qian
- Hongda Jiang
- Haiyang Yu
- Liyao Sun
- Xi Wei
- Yunduan Sun
- Hongyan Ge
- Haizhou Zhou
- Xiaoguang Li
- Takashi Hashimoto
- Xianling Tang
- Ping Liu
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Affiliations: Eye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Academician Workstation, Harbin Medical University and Heilongjiang Academy of Medical Sciences, Harbin, Heilongjiang 150081, P.R. China, Department of Laboratory Diagnosis, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka 545‑8585, Japan

Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5726-5733
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Published online on: February 8, 2018

https://doi.org/10.3892/mmr.2018.8581
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Abstract

Cataracts are the most common eye disease to cause blindness in patients. The abnormal deposition of laminins (LMs) in the lens capsule and the disruption of capsular epithelium contribute to cataract development, although the mechanism by which this occurs is currently unclear. The present study aimed to reproduce HLE B‑3 basement membranes (BMs) using HLE B‑3 cells and to analyze the similarities of LM expression between HLE B‑3 BMs and human anterior lens capsule (ALC). Immunohistochemistry (IHC), ELISA, western blot analysis and immunoprecipitation (IP)‑western blot analysis were used to detect total LMs, LM trimers and 11 LM subunits in HLE B‑3 cells, HLE B‑3 BMs and human ALCs. In IHC staining, HLE B‑3 cells and human ALCs were positive for LMs. In LM ELISA, all samples analyzed were positive for LMs. Western blot analysis detected all LM subunits except for LMγ3 in HLE B‑3 cell lysate, 4 subunits (LMα4, LMα2, LMα1 and LMγ1) in HLE B‑3 cell culture supernatant, 5 subunits (LMα4, LMα2, LMα1, LMβ3 and LMγ1) in HLE B‑3 BMs, and 3 subunits (LMα4, LMγ2 and LMγ1) in human ALCs. The results of IP‑western blot analysis revealed that the LM411 trimer was detected in HLE B‑3 cell culture supernatant. These results indicated that HLE B‑3 BMs were similar to human ALCs in terms of LM expression. Therefore, HLE B‑3 BMs could be used as an in vitro ALC model to determine the role of LMs in ALC in the pathogenesis of cataracts and to select potential anti‑cataract drugs.

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1	Michael R and Bron AJ: The ageing lens and cataract: A model of normal and pathological ageing. Philos Trans R Soc Lond B Biol Sci. 366:1278–1292. 2011. View Article : Google Scholar : PubMed/NCBI
2	Bourne RR, Stevens GA, White RA, Smith JL, Flaxman SR, Price H, Jonas JB, Keeffe J, Leasher J, Naidoo K, et al: Causes of vision loss worldwide, 1990–2010: A systematic analysis. Lancet Glob Health. 1:e339–e349. 2013. View Article : Google Scholar : PubMed/NCBI
3	Khairallah M, Kahloun R, Bourne R, Limburg H, Flaxman SR, Jonas JB, Keeffe J, Leasher J, Naidoo K, Pesudovs K, et al: Number of people blind or visually impaired by cataract worldwide and in world regions, 1990 to 2010. Invest Ophthalmol Vis Sci. 56:6762–6279. 2015. View Article : Google Scholar : PubMed/NCBI
4	Pollreisz A and Schmidt-Erfurth U: Diabetic cataract-pathogenesis, epidemiology and treatment. J Ophthalmol. 2010:6087512010. View Article : Google Scholar : PubMed/NCBI
5	Roberts JE: Ultraviolet radiation as a risk factor for cataract and macular degeneration. Eye Contact Lens. 37:246–249. 2011. View Article : Google Scholar : PubMed/NCBI
6	Asbell PA, Dualan I, Mindel J, Brocks D, Ahmad M and Epstein S: Age-related cataract. Lancet. 365:599–609. 2005. View Article : Google Scholar : PubMed/NCBI
7	Rao GN, Khanna R and Payal A: The global burden of cataract. Curr Opin Ophthalmol. 22:4–9. 2011. View Article : Google Scholar : PubMed/NCBI
8	Bhat SP: The ocular lens epithelium. Biosci Rep. 21:537–563. 2001. View Article : Google Scholar : PubMed/NCBI
9	Li WC, Kuszak JR, Dunn K, Wang RR, Ma W, Wang GM, Spector A, Leib M, Cotliar AM, Weiss M, et al: Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals. J Cell Biol. 130:169–181. 1995. View Article : Google Scholar : PubMed/NCBI
10	Martinez G and de Iongh RU: The lens epithelium in ocular health and disease. Int J Biochem Cell Biol. 42:1945–1963. 2010. View Article : Google Scholar : PubMed/NCBI
11	Schéele S, Nyström A, Durbeej M, Talts JF, Ekblom M and Ekblom P: Laminin isoforms in development and disease. J Mol Med (Berl). 85:825–836. 2007. View Article : Google Scholar : PubMed/NCBI
12	Durbeej M: Laminins. Cell Tissue Res. 339:259–268. 2010. View Article : Google Scholar : PubMed/NCBI
13	Yurchenco PD and Patton BL: Developmental and pathogenic mechanisms of basement membrane assembly. Curr Pharm Des. 15:1277–1294. 2009. View Article : Google Scholar : PubMed/NCBI
14	Miner JH and Yurchenco PD: Laminin functions in tissue morphogenesis. Annu Res Cell Dev Biol. 20:255–284. 2004. View Article : Google Scholar
15	Byström B, Virtanen I, Rousselle P, Gullberg D and Pedrosa-Domellöf F: Distribution of laminins in the developing human eye. Invest Ophthalmol Vis Sci. 47:777–785. 2006. View Article : Google Scholar : PubMed/NCBI
16	Kohno T, Sorgente N, Ishibashi T, Goodnight R and Ryan SJ: Immunofluorescent studies of fibronectin and laminin in the human eye. Invest Ophthalmol Vis Sci. 28:506–514. 1987.PubMed/NCBI
17	Uechi G, Sun Z, Schreiber EM, Halfter W and Balasubramani M: Proteomic view of basement membranes from human retinal blood vessels, inner limiting membranes, and lens capsules. J Proteome Res. Jul 17–2014.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
18	Danysh BP and Duncan MK: The lens capsule. Exp Eye Res. 88:151–164. 2009. View Article : Google Scholar : PubMed/NCBI
19	Zenker M, Tralau T, Lennert T, Pitz S, Mark K, Madlon H, Dötsch J, Reis A, Müntefering H and Neumann LM: Congenital nephrosis, mesangial sclerosis and distinct eye abnormalities with microcoria: An autosomal recessive syndrome. Am J Med Genet A. 130A:1–145. 2004. View Article : Google Scholar
20	Stunf S, Hvala A, Vidovič Valentinčič N, Kraut A and Hawlina M: Ultrastructure of the anterior lens capsule and epithelium in cataracts associated with uveitis. Ophthalmic Res. 48:12–21. 2012. View Article : Google Scholar : PubMed/NCBI
21	Suh J, Moncaster JA, Wang L, Hafeez I, Herz J, Tanzi RE, Goldstein LE and Guénette SY: FE65 and FE65L1 amyloid precursor protein-binding protein compound null mice display adult-onset cataract and muscle weakness. FASEB J. 29:2628–2639. 2015. View Article : Google Scholar : PubMed/NCBI
22	Joo CK, Lee EH, Kim JC, Kim YH, Lee JH, Kim JT, Chung KH and Kim J: Degeneration and transdifferentiation of human lens epithelial cells in nuclear and anterior polar cataracts. J Cataract Refract Surg. 25:652–658. 1999. View Article : Google Scholar : PubMed/NCBI
23	de Iongh RU, Wederell E, Lovicu FJ and McAvoy JW: Transforming growth factor-beta-induced epithelial-mesenchymal transition in the lens: A model for cataract formation. Cells Tissues Organs. 179:43–55. 2005. View Article : Google Scholar : PubMed/NCBI
24	Arita T, Murata Y, Lin LR, Tsuji T and Reddy VN: Synthesis of lens capsule in long-term culture of human lens epithelial cells. Invest Ophthalmol Vis Sci. 34:355–362. 1993.PubMed/NCBI
25	Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y and Owaribe K: Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line. Exp Cell Res. 324:172–182. 2014. View Article : Google Scholar : PubMed/NCBI
26	Li X, Qian H, Sogame R, Hirako Y, Tsuruta D, Ishii N, Koga H, Tsuchisaka A, Jin Z, Tsubota K, et al: Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. Eur J Dermatol. 26:247–253. 2016.PubMed/NCBI
27	Andjelic S, Drašlar K, Hvala A and Hawlina M: Anterior lens epithelium in cataract patients with retinitis pigmentosa-scanning and transmission electron microscopy study. Acta Ophthalmol. 95:e212–e220. 2017. View Article : Google Scholar : PubMed/NCBI
28	Li X, Qian H, Takizawa M, Koga H, Tsuchisaka A, Ishii N, Hayakawa T, Ohara K, Sitaru C, Zillikens D, et al: N-linked glycosylation on laminin γ1 influences recognition of anti-laminin γ1 pemphigoid autoantibodies. J Dermatol Sci. 77:125–129. 2015. View Article : Google Scholar : PubMed/NCBI
29	Patrick B, Li J, Jeyabal PV, Reddy PM, Yang Y, Sharma R, Sinha M, Luxon B, Zimniak P, Awasthi S, et al: Depletion of 4-hydroxynonenal in hGSTA4-transfected HLE B-3 cells results in profound changes in gene expression. Biochem Biophys Res Commun. 334:425–432. 2005. View Article : Google Scholar : PubMed/NCBI
30	Sigle RO, Gil SG, Bhattacharya M, Ryan MC, Yang TM, Brown TA, Boutaud A, Miyashita Y, Olerud J and Carter WG: Globular domains 4/5 of the laminin alpha3 chain mediate deposition of precursor laminin 5. J Cell Sci. 117:4481–4494. 2004. View Article : Google Scholar : PubMed/NCBI
31	Frank DE and Carter WG: Laminin 5 deposition regulates keratinocyte polarization and persistent migration. J Cell Sci. 117:1351–1363. 2004. View Article : Google Scholar : PubMed/NCBI
32	Zhang XH, Sun HM and Yuan JQ: Extracellular matrix production of lens epithelial cells. J Cataract Refract Surg. 27:1303–1309. 2001. View Article : Google Scholar : PubMed/NCBI
33	Gonzalez AM, Gonzales M, Herron GS, Nagavarapu U, Hopkinson SB, Tsuruta D and Jones JC: Complex interactions between the laminin alpha 4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo. Proc Natl Acad Sci USA. 99:pp. 16075–16080. 2002; View Article : Google Scholar : PubMed/NCBI
34	Patarroyo M, Tryggvason K and Virtanen I: Laminin isoforms in tumor invasion, angiogenesis and metastasis. Semin Cancer Biol. 12:197–207. 2002. View Article : Google Scholar : PubMed/NCBI
35	Qu H, Liu X, Ni Y, Jiang Y, Feng X, Xiao J, Guo Y, Kong D, Li A, Li X, et al: Laminin 411 acts as a potent inducer of umbilical cord mesenchymal stem cell differentiation into insulin-producing cells. J Transl Med. 12:1352014. View Article : Google Scholar : PubMed/NCBI