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Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis

  • Authors:
    • Yi Wu
    • Carly Siu‑Yin Lam
    • Dennis Yan‑Yin Tse
    • Chi Ho To
    • Quan Liu
    • Sally A. McFadden
    • Rachel Ka‑Man Chun
    • King Kit Li
    • Jianfang Bian
    • Chuen Lam
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑Sen University, Guangzhou, Guangdong 510060, P.R. China, Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong, SAR, P.R. China, School of Psychology, Faculty of Science and IT, The University of Newcastle, Callaghan, NSW 2308, Australia
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5571-5580
    |
    Published online on: February 8, 2018
       https://doi.org/10.3892/mmr.2018.8584
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Abstract

The current study aimed to investigate the differential protein expression in guinea pig retinas in response to lens-induced myopia (LIM) before fully compensated eye growth. Four days old guinea pigs (n=5) were subjected to ‑4D LIM for 8 days. Refractive errors were measured before and at the end of the lens wear period. Ocular dimensions were also recorded using high‑frequency A‑scan ultrasonography. After the LIM treatment, retinas of both eyes were harvested and soluble proteins were extracted. Paired retinal protein expressions in each animal were profiled and compared using a sensitive fluorescence difference two‑dimensional gel electrophoresis. The quantitative retinal proteomes of myopic and control eye were analysed using computerised DeCyder software. Those proteins that were consistently changed with at least 1.2‑fold difference (P<0.05) in the same direction in all five animals were extracted, trypsin digested and identified by tandem mass spectrometry. Significant myopia was induced in guinea pigs after 8 days of lens wear. The vitreous chamber depth in lens‑treated eyes was found to be significantly elongated. Typically, more than 1,000 protein spots could be detected from each retina. Thirty‑two of them showed differential expression between myopic and untreated retina. Among these proteins, 21 spots were upregulated and 11 were downregulated. Eight protein spots could be successfully identified which included β‑actin, enolase 1, cytosolic malate dehydrogenase, Ras‑related protein Rab‑11B, protein‑L‑isoaspartate (D‑aspartate) O‑methyltransferase, PKM2 protein, X‑linked eukaryotic translation initiation factor 1A and ACP1 protein. The present study serves as the first report to uncover the retinal 2D proteome expressions in mammalian guinea pig myopia model using a top‑down fluorescent dyes labelling gel approach. The results showed a downregulation in glycolytic enzymes that may suggest a significant alteration of glycolysis during myopia development. Other protein candidates also suggested multiple pathways which could provide new insights for further study of the myopic eye growth.
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1 

Markoulli M, Papas E, Cole N and Holden B: Differential gel electrophoresis of the tear proteome. Optom Vis Sci. 89:E875–E883. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Joseph R, Srivastava OP and Pfister RR: Differential epithelial and stromal protein profiles in keratoconus and normal human corneas. Exp Eye Res. 92:282–298. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Chowdhury UR, Madden BJ, Charlesworth MC and Fautsch MP: Proteome analysis of human aqueous humor. Invest Ophthalmol Vis Sci. 51:4921–4931. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Tse DY, Lam CS, Guggenheim JA, Lam C, Li KK, Liu Q and To CH: Simultaneous defocus integration during refractive development. Invest Ophthalmol Vis Sci. 48:5352–5359. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Wallman J, Turkel J and Trachtman J: Extreme myopia produced by modest change in early visual experience. Science. 201:1249–1251. 1978. View Article : Google Scholar : PubMed/NCBI

6 

Norton TT: Experimental myopia in tree shrews. Ciba Found Symp. 155:178–199. 1990.PubMed/NCBI

7 

Shen W, Vijayan M and Sivak JG: Inducing form-deprivation myopia in fish. Invest Ophthalmol Vis Sci. 46:1797–1803. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Barathi VA, Boopathi VG, Yap EP and Beuerman RW: Two models of experimental myopia in the mouse. Vision Res. 48:904–916. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Bradley DV, Fernandes A, Lynn M, Tigges M and Boothe RG: Emmetropization in the rhesus monkey (Macaca mulatta): Birth to young adulthood. Invest Ophthalmol Vis Sci. 40:214–229. 1999.PubMed/NCBI

10 

Troilo D, Nickla DL and Wildsoet CF: Form deprivation myopia in mature common marmosets (Callithrix jacchus). Invest Ophthalmol Vis Sci. 41:2043–2049. 2000.PubMed/NCBI

11 

Howlett MH and McFadden SA: Form-deprivation myopia in the guinea pig (Cavia porcellus). Vision Res. 46:267–283. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Robb RM: Refractive errors associated with hemangiomas of the eyelids and orbit in infancy. Am J Ophthalmol. 83:52–58. 1977. View Article : Google Scholar : PubMed/NCBI

13 

O'Leary DJ and Millodot M: Eyelid closure causes myopia in humans. Experientia. 35:1478–1479. 1979. View Article : Google Scholar : PubMed/NCBI

14 

Hoyt CS, Stone RD, Fromer C and Billson FA: Monocular axial myopia associated with neonatal eyelid closure in human infants. Am J Ophthalmol. 91:197–200. 1981. View Article : Google Scholar : PubMed/NCBI

15 

Johnson CA, Post RB, Chalupa LM and Lee TJ: Monocular deprivation in humans: A study of identical twins. Invest Ophthalmol Vis Sci. 23:135–138. 1982.PubMed/NCBI

16 

Wallman J and Winawer J: Homeostasis of eye growth and the question of myopia. Neuron. 43:447–468. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Morgan IG: The biological basis of myopic refractive error. Clin Exp Optom. 86:276–288. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Wildsoet C and Wallman J: Choroidal and scleral mechanisms of compensation for spectacle lenses in chicks. Vision Res. 35:1175–1194. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Troilo D, Gottlieb MD and Wallman J: Visual deprivation causes myopia in chicks with optic nerve section. Curr Eye Res. 6:993–999. 1987. View Article : Google Scholar : PubMed/NCBI

20 

Lam TC, Li KK, Lo SC, Guggenheim JA and To CH: Application of fluorescence difference gel electrophoresis technology in searching for protein biomarkers in chick myopia. J Proteome Res. 6:4135–4149. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Lam TC, Li KK, Lo SC, Guggenheim JA and To CH: A chick retinal proteome database and differential retinal protein expressions during early ocular development. J Proteome Res. 5:771–784. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Bertrand E, Fritsch C, Diether S, Lambrou G, Müller D, Schaeffel F, Schindler P, Schmid KL, van Oostrum J and Voshol H: Identification of apolipoprotein A-I as a ‘STOP’ signal for myopia. Mol Cell Proteomics. 5:2158–2166. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Wu Y, Liu Q, To CH, Li KK, Chun RKM, Yu JFJ and Lam TC: Differential retinal protein expressions during form deprivation myopia in albino guinea pigs. Curr Proteomics. 11:37–47. 2014. View Article : Google Scholar

24 

Leotta AJ, Bowrey HE, Zeng G and McFadden SA: Temporal properties of the myopic response to defocus in the guinea pig. Ophthalmic Physiol Opt. 33:227–244. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Howlett MH and McFadden SA: Spectacle lens compensation in the pigmented guinea pig. Vision Res. 49:219–227. 2009. View Article : Google Scholar : PubMed/NCBI

26 

McFadden SA, Tse DY, Bowrey HE, Leotta AJ, Lam CS, Wildsoet CF and To CH: Integration of defocus by dual power Fresnel lenses inhibits myopia in the mammalian eye. Invest Ophthalmol Vis Sci. 55:908–917. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Howlett MH and McFadden SA: Emmetropization and schematic eye models in developing pigmented guinea pigs. Vision Res. 47:1178–1190. 2007. View Article : Google Scholar : PubMed/NCBI

28 

McFadden SA, Howlett MH and Mertz JR: Retinoic acid signals the direction of ocular elongation in the guinea pig eye. Vision Res. 44:643–653. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Tonge R, Shaw J, Middleton B, Rowlinson R, Rayner S, Young J, Pognan F, Hawkins E, Currie I and Davison M: Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics. 1:377–396. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Frost MR and Norton TT: Alterations in protein expression in tree shrew sclera during development of lens-induced myopia and recovery. Invest Ophthalmol Vis Sci. 53:322–336. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Comi GP, Fortunato F, Lucchiari S, Bordoni A, Prelle A, Jann S, Keller A, Ciscato P, Galbiati S, Chiveri L, et al: Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis. Ann Neurol. 50:202–207. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Lo AS, Liew CT, Ngai SM, Tsui SK, Fung KP, Lee CY and Waye MM: Developmental regulation and cellular distribution of human cytosolic malate dehydrogenase (MDH1). J Cell Biochem. 94:763–773. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Muto A, Arai K and Watanabe S: Rab11-FIP4 is predominantly expressed in neural tissues and involved in proliferation as well as in differentiation during zebrafish retinal development. Dev Biol. 292:90–102. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Kim E, Lowenson JD, Clarke S and Young SG: Phenotypic analysis of seizure-prone mice lacking L-isoaspartate (D-aspartate) O-methyltransferase. J Biol Chem. 274:20671–20678. 1999. View Article : Google Scholar : PubMed/NCBI

35 

Morohoshi K, Ohbayashi M, Patel N, Chong V, Bird AC and Ono SJ: Identification of anti-retinal antibodies in patients with age-related macular degeneration. Exp Mol Pathol. 93:193–199. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Lindsay K, Linton J and Hurley J: Unique expression and regulation of glycolytic enzyme PKM2 in photoreceptor cells and the role of enzymatic activity modulating metabolism of the retina. Invest Ophthalmol Vis Sci. 54:6922013.

37 

Chaudhuri J, Si K and Maitra U: Function of eukaryotic translation initiation factor 1A (eIF1A) (formerly called eIF-4C) in initiation of protein synthesis. J Biol Chem. 272:7883–7891. 1997. View Article : Google Scholar : PubMed/NCBI

38 

Majumdar R, Bandyopadhyay A and Maitra U: Mammalian translation initiation factor eIF1 functions with eIF1A and eIF3 in the formation of a stable 40 S preinitiation complex. J Biol Chem. 278:6580–6587. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Kainuma M and Hershey JW: Depletion and deletion analyses of eucaryotic translation initiation factor 1A in Saccharomyces cerevisiae. Biochimie. 83:505–514. 2001. View Article : Google Scholar : PubMed/NCBI

40 

Chiarugi P, Taddei ML, Schiavone N, Papucci L, Giannoni E, Fiaschi T, Capaccioli S, Raugei G and Ramponi G: LMW-PTP is a positive regulator of tumor onset and growth. Oncogene. 23:3905–3914. 2004. View Article : Google Scholar : PubMed/NCBI

41 

Iannaccone U, Bergamaschi A, Magrini A, Marino G, Bottini N, Lucarelli P, Bottini E and Gloria-Bottini F: Serum glucose concentration and ACP1 genotype in healthy adult subjects. Metabolism. 54:891–894. 2005. View Article : Google Scholar : PubMed/NCBI

42 

Lucarini N, Bottini N, Antonacci E, Borgiani P, Faggioni G and Gloria-Bottini F: Diabetic complications and the genetics of signal transduction. A study of retinopathy in NIDDM. Dis Markers. 13:169–176. 1997.PubMed/NCBI

43 

Lu F, Zhou X, Jiang L, Fu Y, Lai X, Xie R and Qu J: Axial myopia induced by hyperopic defocus in guinea pigs: A detailed assessment on susceptibility and recovery. Exp Eye Res. 89:101–108. 2009. View Article : Google Scholar : PubMed/NCBI

44 

Lu F, Zhou X, Zhao H, Wang R, Jia D, Jiang L, Xie R and Qu J: Axial myopia induced by a monocularly-deprived facemask in guinea pigs: A non-invasive and effective model. Exp Eye Res. 82:628–636. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Mazurek S, Boschek CB, Hugo F and Eigenbrodt E: Pyruvate kinase type M2 and its role in tumor growth and spreading. Semin Cancer Biol. 15:300–308. 2005. View Article : Google Scholar : PubMed/NCBI

46 

Mazurek S: Pyruvate kinase type M2: A key regulator of the metabolic budget system in tumor cells. Int J Biochem Cell Biol. 43:969–980. 2011. View Article : Google Scholar : PubMed/NCBI

47 

Tan SQ, Geng X, Liu JH, Pan WH, Wang LX, Liu HK, Hu L and Chao HM: Xue-fu-Zhu-Yu decoction protects rats against retinal ischemia by downregulation of HIF-1α and VEGF via inhibition of RBP2 and PKM2. BMC Complement Altern Med. 17:3652017. View Article : Google Scholar : PubMed/NCBI

48 

López-Alemany R, Longstaff C, Hawley S, Mirshahi M, Fábregas P, Jardí M, Merton E, Miles LA and Félez J: Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase. Am J Hematol. 72:234–242. 2003. View Article : Google Scholar : PubMed/NCBI

49 

Jin ZB, Wu J, Huang XF, Feng CY, Cai XB, Mao JY, Xiang L, Wu KC, Xiao X, Kloss BA, et al: Trio-based exome sequencing arrests de novo mutations in early-onset high myopia. Proc Natl Acad Sci USA. 114:pp. 4219–4224. 2017; View Article : Google Scholar : PubMed/NCBI

50 

Souda P, Ryan CM, Cramer WA and Whitelegge J: Profiling of integral membrane proteins and their post translational modifications using high-resolution mass spectrometry. Methods. 55:330–336. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Chun RKM, Tse DYY, Zuo B, Li KK, Zhang G, Liu Q, Zhang SA and To CH: Proteome analysis of guinea pig retina during recovery from lens-induced myopia. Invest Ophthalmol Vis Sci. 52:56462011.

52 

Dunker S, Sadun AA and Sebag J: Neuron specific enolase in retinal detachment. Curr Eye Res. 23:382–385. 2001. View Article : Google Scholar : PubMed/NCBI

53 

Quintyn JC, Pereira F, Hellot MF, Brasseur G and Coquerel A: Concentration of neuron-specific enolase and S100 protein in the subretinal fluid of rhegmatogenous retinal detachment. Graefes Arch Clin Exp Ophthalmol. 243:1167–1174. 2005. View Article : Google Scholar : PubMed/NCBI

54 

Feldkaemper MP, Neacsu I and Schaeffel F: Insulin acts as a powerful stimulator of axial myopia in chicks. Invest Ophthalmol Vis Sci. 50:13–23. 2009. View Article : Google Scholar : PubMed/NCBI

55 

Tarutta E, Chua WH, Young T, Goldschmidt E, Saw SM, Rose KA, Smith E III, Mutti DO, Ashby R, Stone RA, et al: Myopia: Why study the mechanisms of myopia? Novel approaches to risk factors signalling eye growth-how could basic biology be translated into clinical insights? Where are genetic and proteomic approaches leading? How does visual function contribute to and interact with Ametropia? Does eye shape matter? Why Ametropia at all? Optom Vis Sci. 88:404–447. 2011. View Article : Google Scholar

56 

Cordain L, Eaton SB, Brand Miller J, Lindeberg S and Jensen C: An evolutionary analysis of the aetiology and pathogenesis of juvenile-onset myopia. Acta Ophthalmol Scand. 80:125–135. 2002. View Article : Google Scholar : PubMed/NCBI

57 

Maccari R and Ottanà R: Low molecular weight phosphotyrosine protein phosphatases as emerging targets for the design of novel therapeutic agents. J Med Chem. 55:2–22. 2012. View Article : Google Scholar : PubMed/NCBI

58 

Liu SZ, Wen D, Mao JF, Tan XP, Xia ZH and Fu CY: The expression of NMDAR1 in the retina of guinea pigs with form-deprivation myopia. Chin J Optom Ophthalmol. 10:1–5. 2008.(In Chinese).

59 

Zhou X, Ye J, Willcox MD, Xie R, Jiang L, Lu R, Shi J, Bai Y and Qu J: Changes in protein profiles of guinea pig sclera during development of form deprivation myopia and recovery. Mol Vis. 16:2163–2174. 2010.PubMed/NCBI

60 

Unlu M, Morgan ME and Minden JS: Difference gel electrophoresis: A single gel method for detecting changes in protein extracts. Electrophoresis. 18:2071–2077. 1997. View Article : Google Scholar : PubMed/NCBI

61 

Lam TC, Chun RK, Li KK and To CH: Snapshots for intra-and inter-ocular differences at retinal proteins levels. Int J Ophthalmol Eye Res. 2:70–76. 2014.

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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Lam CS, Tse DY, To CH, Liu Q, McFadden SA, Chun RK, Li KK, Bian J, Lam C, Lam C, et al: Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis. Mol Med Rep 17: 5571-5580, 2018.
APA
Wu, Y., Lam, C.S., Tse, D.Y., To, C.H., Liu, Q., McFadden, S.A. ... Lam, C. (2018). Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis. Molecular Medicine Reports, 17, 5571-5580. https://doi.org/10.3892/mmr.2018.8584
MLA
Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C."Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis". Molecular Medicine Reports 17.4 (2018): 5571-5580.
Chicago
Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C."Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis". Molecular Medicine Reports 17, no. 4 (2018): 5571-5580. https://doi.org/10.3892/mmr.2018.8584
Copy and paste a formatted citation
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Spandidos Publications style
Wu Y, Lam CS, Tse DY, To CH, Liu Q, McFadden SA, Chun RK, Li KK, Bian J, Lam C, Lam C, et al: Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis. Mol Med Rep 17: 5571-5580, 2018.
APA
Wu, Y., Lam, C.S., Tse, D.Y., To, C.H., Liu, Q., McFadden, S.A. ... Lam, C. (2018). Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis. Molecular Medicine Reports, 17, 5571-5580. https://doi.org/10.3892/mmr.2018.8584
MLA
Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C."Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis". Molecular Medicine Reports 17.4 (2018): 5571-5580.
Chicago
Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C."Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis". Molecular Medicine Reports 17, no. 4 (2018): 5571-5580. https://doi.org/10.3892/mmr.2018.8584
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