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Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein

  • Authors:
    • Yunzhong Zhang
    • Jing Feng
    • Jizhen Cui
    • Guozheng Yang
    • Xianai Zhu
  • View Affiliations / Copyright

    Affiliations: Intensive Care Unit, Qingzhou Hospital, Taishan Medical University, Qingzhou, Shandong 262500, P.R. China, Department of Gynecology, Qingzhou Hospital, Taishan Medical University, Qingzhou, Shandong 262500, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5805-5813
    |
    Published online on: February 15, 2018
       https://doi.org/10.3892/mmr.2018.8609
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Abstract

The present study investigated the roles of pre-B cell leukemia transcription factor 3 (PBX3) in sepsis. Reverse transcription-quantitative polymerase chain reaction and western blot analysis indicated that overexpression of the PBX 3'‑untranslated region (UTR) promoted high mobility group box 1 (HMGB1) protein expression in human umbilical vein endothelial cells (HUVECs) (P<0.01). Furthermore, post‑treatment of PBX3 small interfering (si)RNA suppressed lipopolysaccharide (LPS)‑mediated HMGB1 release and attenuated HMGB1‑mediated hyperpermeability and leukocyte migration in HUVECs and septic mice (P<0.01). Additionally, post‑injection of PBX3 siRNA also induced the downregulation of cecal ligation and puncture‑induced HMGB1 release, production of IL‑6 and mortality (P<0.01). Mechanistically, the 3'UTRs of PBX3 and HMGB1 were identified to harbor six common micro (mi)RNA binding sites, and PBX 3'UTR increased HMGB1 expression in a 3'UTR‑ and miRNA‑dependent manner. Notably, the coding sequence of PBX3 did not increase HMGB1 expression in HUVECs. Collectively, the present study indicates that PBX 3'UTR may induce inflammatory responses and sepsis via acting as a competing endogenous RNA for HMGB1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Feng J, Cui J, Yang G and Zhu X: Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Mol Med Rep 17: 5805-5813, 2018.
APA
Zhang, Y., Feng, J., Cui, J., Yang, G., & Zhu, X. (2018). Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Molecular Medicine Reports, 17, 5805-5813. https://doi.org/10.3892/mmr.2018.8609
MLA
Zhang, Y., Feng, J., Cui, J., Yang, G., Zhu, X."Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein". Molecular Medicine Reports 17.4 (2018): 5805-5813.
Chicago
Zhang, Y., Feng, J., Cui, J., Yang, G., Zhu, X."Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein". Molecular Medicine Reports 17, no. 4 (2018): 5805-5813. https://doi.org/10.3892/mmr.2018.8609
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Feng J, Cui J, Yang G and Zhu X: Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Mol Med Rep 17: 5805-5813, 2018.
APA
Zhang, Y., Feng, J., Cui, J., Yang, G., & Zhu, X. (2018). Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Molecular Medicine Reports, 17, 5805-5813. https://doi.org/10.3892/mmr.2018.8609
MLA
Zhang, Y., Feng, J., Cui, J., Yang, G., Zhu, X."Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein". Molecular Medicine Reports 17.4 (2018): 5805-5813.
Chicago
Zhang, Y., Feng, J., Cui, J., Yang, G., Zhu, X."Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein". Molecular Medicine Reports 17, no. 4 (2018): 5805-5813. https://doi.org/10.3892/mmr.2018.8609
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