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Effects of sheep/goat whey protein dietary supplementation on the redox status of rats

  • Authors:
    • Efthalia Kerasioti
    • Dimitrios Stagos
    • Aristides M. Tsatsakis
    • Demetrios A. Spandidos
    • Ioannis Taitzoglou
    • Demetrios Kouretas
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece, Department of Forensic Sciences and Toxicology, Medical School, University of Crete, 71409 Heraklion, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
    Copyright: © Kerasioti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5774-5781
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    Published online on: February 20, 2018
       https://doi.org/10.3892/mmr.2018.8622
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Abstract

The purpose of the present study is to estimate the effects of sheep/goat whey protein dietary supplementation on the redox status of blood and tissues of rats. Twelve male Wistar rats were divided into the control group (standard commercial diet) and whey group [standard commercial diet + sheep/goat whey protein (1 g kg b.w/day)] (6 rats/group). The animals were maintainted on their respective diet for 28 days. At the end of the experimental period, reduced glutathione, catalase activity, total antioxidant capacity, thiobarbituric reactive substances, protein carbonyls and the decomposition rate of H2O2 were measured in blood and tissues of rats. According to the results, the rats fed with the sheep/goat whey protein exhibited improved antioxidant status and decreased free radical‑induced toxic effects on lipids and proteins. Specifically, in blood, GSH and CAT levels were significantly increased while TBARS and protein carbonyl levels were significantly decreased compared to the control group. Regarding the effects on tissues, it was observed that GSH levels were significantly increased in small intestine, quadriceps muscle, pancreas and lung tissue compared to the control group. The decomposition rate of H2O2 was significantly decreased in liver, brain and quadriceps muscle, but was significantly increased in spleen tissue compared to the control group. TBARS levels were significantly decreased in liver, brain, quadriceps muscle, pancreas, lung and spleen tissue compared to the control group. Finally, protein carbonyl levels were significantly decreased in brain, small intestine, kidney, pancreas and spleen tissue compared to the control group. Thus, the present findings show the beneficial effects of sheep/goat whey protein, a by‑product of cheese manufacturing, on the redox status in an in vivo model.
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Copy and paste a formatted citation
Spandidos Publications style
Kerasioti E, Stagos D, Tsatsakis AM, Spandidos DA, Taitzoglou I and Kouretas D: Effects of sheep/goat whey protein dietary supplementation on the redox status of rats. Mol Med Rep 17: 5774-5781, 2018.
APA
Kerasioti, E., Stagos, D., Tsatsakis, A.M., Spandidos, D.A., Taitzoglou, I., & Kouretas, D. (2018). Effects of sheep/goat whey protein dietary supplementation on the redox status of rats. Molecular Medicine Reports, 17, 5774-5781. https://doi.org/10.3892/mmr.2018.8622
MLA
Kerasioti, E., Stagos, D., Tsatsakis, A. M., Spandidos, D. A., Taitzoglou, I., Kouretas, D."Effects of sheep/goat whey protein dietary supplementation on the redox status of rats". Molecular Medicine Reports 17.4 (2018): 5774-5781.
Chicago
Kerasioti, E., Stagos, D., Tsatsakis, A. M., Spandidos, D. A., Taitzoglou, I., Kouretas, D."Effects of sheep/goat whey protein dietary supplementation on the redox status of rats". Molecular Medicine Reports 17, no. 4 (2018): 5774-5781. https://doi.org/10.3892/mmr.2018.8622
Copy and paste a formatted citation
x
Spandidos Publications style
Kerasioti E, Stagos D, Tsatsakis AM, Spandidos DA, Taitzoglou I and Kouretas D: Effects of sheep/goat whey protein dietary supplementation on the redox status of rats. Mol Med Rep 17: 5774-5781, 2018.
APA
Kerasioti, E., Stagos, D., Tsatsakis, A.M., Spandidos, D.A., Taitzoglou, I., & Kouretas, D. (2018). Effects of sheep/goat whey protein dietary supplementation on the redox status of rats. Molecular Medicine Reports, 17, 5774-5781. https://doi.org/10.3892/mmr.2018.8622
MLA
Kerasioti, E., Stagos, D., Tsatsakis, A. M., Spandidos, D. A., Taitzoglou, I., Kouretas, D."Effects of sheep/goat whey protein dietary supplementation on the redox status of rats". Molecular Medicine Reports 17.4 (2018): 5774-5781.
Chicago
Kerasioti, E., Stagos, D., Tsatsakis, A. M., Spandidos, D. A., Taitzoglou, I., Kouretas, D."Effects of sheep/goat whey protein dietary supplementation on the redox status of rats". Molecular Medicine Reports 17, no. 4 (2018): 5774-5781. https://doi.org/10.3892/mmr.2018.8622
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