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Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells

  • Authors:
    • Yingjuan Zheng
    • Chao Zhao
    • Naijian Zhang
    • Wenqin Kang
    • Rongrong Lu
    • Huadong Wu
    • Yingxue Geng
    • Yaping Zhao
    • Xiaoyan Xu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shu Yang Union Medical College Hospital, Suqian, Jiangsu 223600, P.R. China, Department of Clinical Laboratory, The 82nd Hospital of Chinese People's Liberation Army, Huaian, Jiangsu 223001, P.R. China, Department of Postgraduates, BengBu Medical College, Bengbu, Anhui 233004, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5635-5641
    |
    Published online on: February 26, 2018
       https://doi.org/10.3892/mmr.2018.8633
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Abstract

The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the effects of hyperthyroidism on the serum levels of the microRNA (miR) miR‑206 and the role of miR‑206 on TH‑regulated lipid metabolism in liver cells. Serum was obtained from 12 patients diagnosed with hyperthyroidism and 10 healthy control subjects. Human hepatoblastoma (HepG2) cells were used to study the effects of triiodothyronine (T3) and miR‑206 on lipid metabolism. Expression of miR‑206 in serum and cells was determined by reverse transcription‑quantitative polymerase chain reaction analysis. Lipid accumulation in HepG2 cells was assessed with Oil Red O staining. Suppression or overexpression of miR‑206 was performed via transfection with a miR‑206 mimic or miR‑206 inhibitor. Serum miR‑206 was significantly decreased in patients with hyperthyroidism compared with euthyroid controls. Treatment of HepG2 cells with T3 led to reduced total cholesterol (TC) and triglyceride (TG) content, accompanied by reduced miR‑206 expression. Inhibition of endogenous miR‑206 expression decreased intracellular TG and TC content in HepG2 cells. By contrast, overexpression of miR‑206 in HepG2 partially prevented the reduction in TG content induced by treatment with T3. In conclusion, serum miR‑206 expression is reduced in patients with hyperthyroidism. In addition, miR‑206 is involved in T3‑mediated regulation of lipid metabolism in HepG2 cells, indicating a role for miR‑206 in thyroid hormone‑induced disorders of lipid metabolism in the liver.
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1 

Cooper D: Hyperthyroidism. Lancet. 362:459–468. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Boelaert K and Franklyn JA: Thyroid hormone in health and disease. J Endocrinol. 187:1–15. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Oppenheimer JH, Schwartz HL, Lane JT and Thompson MP: Functional relationship of thyroid hormone-induced lipogenesis, lipolysis and thermogenesis in rat. J Clin Invest. 87:125–132. 1991. View Article : Google Scholar : PubMed/NCBI

4 

Kong YW, Cannell IG, de Moor CH, Hill K, Garside PG, Hamilton TL, Meijer HA, Dobbyn HC, Stoneley M, Spriggs KA, et al: The mechanism of micro-rna-mediated translation repression is determined by the promoter of the target gene. Proc Nail Acad Sci USA. 105:pp. 8866–8871. 2008; View Article : Google Scholar

5 

Chen XM: MicroRNA signatures in liver diseases. World J Gastroentero. 15:1665–1672. 2009. View Article : Google Scholar

6 

Lynn FC: Meta-regulation: microRNA regulation of glucose and lipid metabolism. Trends Endocrinol Metab. 20:452–459. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Dong H, Paquette M, Williams A, Zoeller RT, Wade M and Yauk C: Thyroid hormone may regulate mRNA abundance in liver by acting on micrornas. PLoS One. 5:e121362010. View Article : Google Scholar : PubMed/NCBI

8 

Visser WE, Heemstra KA, Swagemakers SM, Ozgür Z, Corssmit EP, Burggraaf J, VanIjcken WF, vanderSpek PJ, Smit JW and Visser TJ: Physiological thyroid hormone levels regulate numerous skeletal muscle transcripts. J Clin Endocr Metab. 94:3487–3496. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Moore KJ, Rayner KJ, Suárez Y and Fernández-Hernando C: The role of microRNAs in cholesterol efflux and hepatic lipid metabolism. Annu Rev Nutr. 31:49–63. 2011. View Article : Google Scholar : PubMed/NCBI

10 

López-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep g2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009. View Article : Google Scholar

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Brent GA: Mechanisms of thyroid hormone action. J Clin Invest. 122:3035–3043. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Iwen KA, Schröder E and Brabant G: Thyroid hormones and the metabolic syndrome. Eur Thyroid J. 2:83–92. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Webb P: Thyroid hormone receptor and lipid regulation. Curr Opin Invest Drugs. 11:1135–1142. 2010.

15 

Shoemaker TJ, Kono T, Mariash CN and Evansmolina C: Thyroid hormone analogues for the treatment of metabolic disorders: New potential for unmet clinical needs? Endocr Pract. 18:954–964. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Cheng SY, Leonard JL and Davis PJ: Molecular aspects of thyroid hormone actions. Endocr Rev. 31:139–170. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Cordeiro A, deSouza LL, Oliveira LS, Faustino LC, Santiago LA, Bloise FF, Ortiga-Carvalho TM, Almeida NA and Pazos-Moura CC: Thyroid hormone regulation of Sirtuin 1 expression and implications to integrated responses in fasted mice. J Endocrinol. 216:181–193. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Sinha RA, You SH, Zhou J, Siddique MM, Bay BH, Zhu X, Privalsky ML, Cheng SY, Stevens RD, Summers SA, et al: Thyroid hormone stimulates hepatic lipid catabolism via activation of autophagy. J Clin Invest. 122:2428–2438. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Yap CS, Sinha RA, Ota S, Katsuki M and Yen PM: Thyroid hormone negatively regulates CDX2 and SOAT2 mRNA expression via induction of miRNA-181d in hepatic cells. Biochen Bioph Res Commun. 440:635–639. 2013. View Article : Google Scholar

20 

Ståhlberg N, Merino R, Hernández LH, Fernández-Pérez L, Sandelin A, Engström P, Tollet-Egnell P, Lenhard B and Flores-Morales A: Exploring hepatic hormone actions using a compilation of gene expression profiles. BMC Physiol. 5:82005. View Article : Google Scholar : PubMed/NCBI

21 

Dong H, Yauk CL, Williams A, Lee A, Douglas GR and Wade MG: Hepatic gene expression changes in hypothyroid juvenile mice: Characterization of a novel negative thyroid-responsive element. Endocrinology. 148:3932–3940. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Shibata A, Kawakami Y, Kimura T, Miyazawa T and Nakagawa K: α-tocopherol attenuates the triglyceride- and cholesterol-lowering effects of rice bran tocotrienol in rats fed a western diet. J Agr Food Chem. 64:5361–5366. 2016. View Article : Google Scholar

23 

Goldberg IJ, Huang LS, Huggins LA, Yu S, Nagareddy PR, Scanlan TS and Ehrenkranz JR: Thyroid hormone reduces cholesterol via a non-LDL receptor-mediated pathway. Endocrinology. 153:5143–5149. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Vickers KC, Sethupathy P, Baran-Gale J and Remaley AT: Complexity of microRNA function and the role of isomiRs in lipid homeostasis. J Lipid Res. 54:1182–1191. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Vickers KC, Shoucri BM, Levin MG, Wu H, Pearson DS, Osei-Hwedieh D, Collins FS, Remaley AT and Sethupathy P: MicroRNA-27b is a regulatory hub in lipid metabolism and is altered in dyslipidemia. Hepatology. 57:533–542. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Zhong D, Huang G, Zhang Y, Zeng Y, Xu Z, Zhao Y, He X and He F: MicroRNA-1 and microRNA-206 suppress LXRα-induced lipogenesis in hepatocytes. Cell Signal. 25:1429–1437. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Vinod M, Chennamsetty I, Colin S, Belloy L, De Paoli F, Schaider H, Graier WF, Frank S, Kratky D, Staels B, et al: MIR-206 controls LXRα expression and promotes LXR-mediated cholesterol efflux in macrophages. Biochim Biophys Acta. 1841:827–835. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zheng Y, Zhao C, Zhang N, Kang W, Lu R, Wu H, Geng Y, Zhao Y and Xu X: Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells. Mol Med Rep 17: 5635-5641, 2018.
APA
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H. ... Xu, X. (2018). Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells. Molecular Medicine Reports, 17, 5635-5641. https://doi.org/10.3892/mmr.2018.8633
MLA
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H., Geng, Y., Zhao, Y., Xu, X."Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells". Molecular Medicine Reports 17.4 (2018): 5635-5641.
Chicago
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H., Geng, Y., Zhao, Y., Xu, X."Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells". Molecular Medicine Reports 17, no. 4 (2018): 5635-5641. https://doi.org/10.3892/mmr.2018.8633
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Y, Zhao C, Zhang N, Kang W, Lu R, Wu H, Geng Y, Zhao Y and Xu X: Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells. Mol Med Rep 17: 5635-5641, 2018.
APA
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H. ... Xu, X. (2018). Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells. Molecular Medicine Reports, 17, 5635-5641. https://doi.org/10.3892/mmr.2018.8633
MLA
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H., Geng, Y., Zhao, Y., Xu, X."Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells". Molecular Medicine Reports 17.4 (2018): 5635-5641.
Chicago
Zheng, Y., Zhao, C., Zhang, N., Kang, W., Lu, R., Wu, H., Geng, Y., Zhao, Y., Xu, X."Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells". Molecular Medicine Reports 17, no. 4 (2018): 5635-5641. https://doi.org/10.3892/mmr.2018.8633
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