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DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway

  • Authors:
    • Li Cong
    • Yong Zhang
    • He Huang
    • Jianguo Cao
    • Xiaohua Fu
  • View Affiliations / Copyright

    Affiliations: Medical College, Hunan Normal University, Changsha, Hunan 410013, P.R. China, Department of Pathology, Maternal and Child Health Hospital of Hunan Province, Changsha, Hunan 410008, P.R. China
    Copyright: © Cong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5692-5699
    |
    Published online on: February 26, 2018
       https://doi.org/10.3892/mmr.2018.8635
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Abstract

7-Difluoromethoxy-5,4'-dimethoxy-genistein (DFMG) is a novel chemical compound synthesized using genistein. Previous studies have indicated that DFMG can reverse the apoptosis of vascular endothelial cells (VECs) by regulating the mitochondrial apoptosis pathway. The present study aimed to investigate the activity and molecular mechanism underlying DFMG‑mediated protection of vascular smooth muscle cell (VSMCs) using a non‑contact co‑culture model established by using Transwell insert. Secretion of interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) were measured by ELISA. Proliferation and migration of VSMCs were assessed using a Cell Counting kit‑8 and wound healing assays, respectively. Toll‑like receptor 4 (TLR4) mRNA and protein levels were detected by reverse transcription-quantitative polymerase chain reaction and western blotting analyses, respectively. In the present study, lysophosphatidylcholine (LPC) significantly increased the secretion of IL‑6 and TNF‑α in VECs. VECs treated with LPC markedly increased proliferation and migration of VSMCs, which were inhibited by DFMG. Transfection of either TLR4 short hairpin RNA (shRNA) or TLR4 cDNA in VECs inhibited and increased proliferation and migration of VSMCs, respectively. Furthermore, transfection of VECs with TLR4 shRNA suppressed the proliferation and migration of VSMCs induced by co‑culture with injured VECs, which was further enhanced by treatment with DFMG. By contrast, transfection of VECs with TLR4 cDNA enhanced proliferation and migration of VSMCs and this effect was inhibited by treatment with DFMG. Taken together, the results of the present study demonstrated that DFMG can reverse proliferation and migration of VSMCs induced by co‑culture with injured VECs via suppression of the TLR4‑mediated signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Cong L, Zhang Y, Huang H, Cao J and Fu X: DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway. Mol Med Rep 17: 5692-5699, 2018.
APA
Cong, L., Zhang, Y., Huang, H., Cao, J., & Fu, X. (2018). DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway. Molecular Medicine Reports, 17, 5692-5699. https://doi.org/10.3892/mmr.2018.8635
MLA
Cong, L., Zhang, Y., Huang, H., Cao, J., Fu, X."DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway". Molecular Medicine Reports 17.4 (2018): 5692-5699.
Chicago
Cong, L., Zhang, Y., Huang, H., Cao, J., Fu, X."DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway". Molecular Medicine Reports 17, no. 4 (2018): 5692-5699. https://doi.org/10.3892/mmr.2018.8635
Copy and paste a formatted citation
x
Spandidos Publications style
Cong L, Zhang Y, Huang H, Cao J and Fu X: DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway. Mol Med Rep 17: 5692-5699, 2018.
APA
Cong, L., Zhang, Y., Huang, H., Cao, J., & Fu, X. (2018). DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway. Molecular Medicine Reports, 17, 5692-5699. https://doi.org/10.3892/mmr.2018.8635
MLA
Cong, L., Zhang, Y., Huang, H., Cao, J., Fu, X."DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway". Molecular Medicine Reports 17.4 (2018): 5692-5699.
Chicago
Cong, L., Zhang, Y., Huang, H., Cao, J., Fu, X."DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway". Molecular Medicine Reports 17, no. 4 (2018): 5692-5699. https://doi.org/10.3892/mmr.2018.8635
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