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Article Open Access

Identification of Der f 23 as a new major allergen of Dermatophagoides farinae

  • Authors:
    • Yongshen He
    • Chuanran Dou
    • Yiming Su
    • Jialin Chen
    • Zhen Zhang
    • Zhenfu Zhao
    • Jiajie Chen
    • Kunmei Ji
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Health Science Center of Shenzhen University, Shenzhen, Guangdong 518060, P.R. China, Shenzhen Vanke Meisha Academy, Shenzhen, Guangdong 518000, P.R. China, The Affiliated International School of Shenzhen University, Shenzhen, Guangdong 518054, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1270-1278
    |
    Published online on: May 28, 2019
       https://doi.org/10.3892/mmr.2019.10305
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Abstract

House dust mites (HDM) are common allergen sources worldwide. At present, 32 of the 37 internationally recognized HDM allergen groups have been identified in Dermatophagoides farinae. The present study study describes the identification of the first known D. farinae Group 23 allergen (Der f 23). Recombinant Der f 23 protein (rDer f 23) was cloned, expressed and purified. The open reading frame of rDer f 23 was 525 base pairs and encoded a 174‑amino acid protein (GenBank accession no., KU166910.1). ELISAs indicated that 72/129 HDM allergic serum samples (55.8%) had specific immunoglobulin E (sIgE) binding activity to rDer f 23. Additionally, 3/10 patients with HDM allergies (30%) exhibited positive skin prick test reactions to rDer f 23. IgE western blot analysis data suggested that only 4/11 HDM allergic sera had a positive sIgE binding result. Sequence homology analysis revealed an extra P2 region (Ser56‑Thr117) in Der f 23 that was not present in the D. pteronyssinus homolog, which may affect sIgE binding. Der f 23ΔP2 demonstrated binding with HDM allergic sera, whereas the P2 peptide alone did not. The sIgE binding ability of Der f 23 ΔP2 (Der f 23 with a truncated P2 region) was more marked compared with that of Der f 23 in an IgE ELISA. These data indicate that P2 region in Der f 23 attenuates IgE binding ability. In conclusion, the results of the present study indicate that Der f 23 is a major HDM allergen with predominantly conformational sIgE binding epitopes. The allergenic identification of Der f 23 and its inclusion in World Health Organization/International Union of Immunological Societies database contributes to the theoretical basis underlying the diagnosis and treatment of HDM allergic diseases.
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Copy and paste a formatted citation
Spandidos Publications style
He Y, Dou C, Su Y, Chen J, Zhang Z, Zhao Z, Chen J and Ji K: Identification of Der f 23 as a new major allergen of Dermatophagoides farinae. Mol Med Rep 20: 1270-1278, 2019.
APA
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z. ... Ji, K. (2019). Identification of Der f 23 as a new major allergen of Dermatophagoides farinae. Molecular Medicine Reports, 20, 1270-1278. https://doi.org/10.3892/mmr.2019.10305
MLA
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z., Chen, J., Ji, K."Identification of Der f 23 as a new major allergen of Dermatophagoides farinae". Molecular Medicine Reports 20.2 (2019): 1270-1278.
Chicago
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z., Chen, J., Ji, K."Identification of Der f 23 as a new major allergen of Dermatophagoides farinae". Molecular Medicine Reports 20, no. 2 (2019): 1270-1278. https://doi.org/10.3892/mmr.2019.10305
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Dou C, Su Y, Chen J, Zhang Z, Zhao Z, Chen J and Ji K: Identification of Der f 23 as a new major allergen of Dermatophagoides farinae. Mol Med Rep 20: 1270-1278, 2019.
APA
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z. ... Ji, K. (2019). Identification of Der f 23 as a new major allergen of Dermatophagoides farinae. Molecular Medicine Reports, 20, 1270-1278. https://doi.org/10.3892/mmr.2019.10305
MLA
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z., Chen, J., Ji, K."Identification of Der f 23 as a new major allergen of Dermatophagoides farinae". Molecular Medicine Reports 20.2 (2019): 1270-1278.
Chicago
He, Y., Dou, C., Su, Y., Chen, J., Zhang, Z., Zhao, Z., Chen, J., Ji, K."Identification of Der f 23 as a new major allergen of Dermatophagoides farinae". Molecular Medicine Reports 20, no. 2 (2019): 1270-1278. https://doi.org/10.3892/mmr.2019.10305
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