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Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells

  • Authors:
    • Liping Wu
    • Lidi Deng
    • Hong Hong
    • Caixia Peng
    • Xueqin Zhang
    • Zhengyuan Chen
    • Junqi Ling
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China, Zhujiang New Town Dental Clinic, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Endodontics, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 939-950
    |
    Published online on: May 29, 2019
       https://doi.org/10.3892/mmr.2019.10308
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Abstract

The dental follicle develops into the periodontal ligament, cementum and alveolar bone. Human dental follicle cells (hDFCs) are the precursor cells of periodontal development. Long non‑coding RNAs (lncRNAs) have been revealed to be crucial factors that regulate a variety of biological processes; however, whether lncRNAs serve a role in human periodontal development remains unknown. Therefore, the present study used microarrays to detect the differentially expressed lncRNAs and mRNAs between hDFCs and human periodontal ligament cells (hPDLCs). A total of 845 lncRNAs and 1,012 mRNAs were identified to be differentially expressed in hDFCs and hPDLCs (fold change >2.0 or <‑2.0; P<0.05). Microarray data were validated by reverse transcription‑quantitative polymerase chain reaction. Bioinformatics analyses, including gene ontology, pathway analysis and coding‑non‑coding gene co‑expression network analysis, were performed to determine the functions of the differentially expressed lncRNAs and mRNAs. Bioinformatics analysis identified that a number of pathways may be associated with periodontal development, including the p53 and calcium signaling pathways. This analysis also revealed a number of lncRNAs, including NR_033932, T152410, ENST00000512129, ENST00000540293, uc021sxs.1 and ENST00000609146, which may serve important roles in the biological process of hDFCs. In addition, the lncRNA termed maternally expressed 3 (MEG3) was identified to be differentially expressed in hDFCs by reverse transcription‑quantitative polymerase chain reaction. The knockdown of MEG3 was associated with a reduction of pluripotency makers in hDFCs. In conclusion, for the first time, to the best of our knowledge, the current study determined the different expression profiles of lncRNAs and mRNAs between hDFCs and hPDLCs. The observations made may provide a solid foundation for further research into the molecular mechanisms of lncRNAs in human periodontal development.
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Copy and paste a formatted citation
Spandidos Publications style
Wu L, Deng L, Hong H, Peng C, Zhang X, Chen Z and Ling J: Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells. Mol Med Rep 20: 939-950, 2019.
APA
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., & Ling, J. (2019). Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells. Molecular Medicine Reports, 20, 939-950. https://doi.org/10.3892/mmr.2019.10308
MLA
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., Ling, J."Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells". Molecular Medicine Reports 20.2 (2019): 939-950.
Chicago
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., Ling, J."Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells". Molecular Medicine Reports 20, no. 2 (2019): 939-950. https://doi.org/10.3892/mmr.2019.10308
Copy and paste a formatted citation
x
Spandidos Publications style
Wu L, Deng L, Hong H, Peng C, Zhang X, Chen Z and Ling J: Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells. Mol Med Rep 20: 939-950, 2019.
APA
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., & Ling, J. (2019). Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells. Molecular Medicine Reports, 20, 939-950. https://doi.org/10.3892/mmr.2019.10308
MLA
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., Ling, J."Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells". Molecular Medicine Reports 20.2 (2019): 939-950.
Chicago
Wu, L., Deng, L., Hong, H., Peng, C., Zhang, X., Chen, Z., Ling, J."Comparison of long non‑coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells". Molecular Medicine Reports 20, no. 2 (2019): 939-950. https://doi.org/10.3892/mmr.2019.10308
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