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A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds

  • Authors:
    • Tiantong Sun
    • Zhentao Man
    • Changliang Peng
    • Guozong Wang
    • Shui Sun
  • View Affiliations / Copyright

    Affiliations: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Orthopedics, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1157-1166
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    Published online on: June 4, 2019
       https://doi.org/10.3892/mmr.2019.10335
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Abstract

Osteonecrosis of the femoral head (ONFH) is a common osteological disease. Treatment of ONFH prior to the collapse of the femoral head is critical for increasing therapeutic efficiency. Tissue engineering therapy using bone mesenchymal stem cells (BMSCs) combined with a scaffold is a promising strategy. However, it is currently unclear how to improve the efficiency of BMSC recruitment under such conditions. In the present study, a specific cyclic peptide for Sprague‑Dawley rat BMSCs, CTTNPFSLC (known as C7), was used, which was identified via phage display technology. Its high affinity for BMSCs was demonstrated using flow cytometry and fluorescence staining. Subsequently, the cyclic peptide was placed on β‑tricalcium phosphate (β‑TCP) scaffolds using absorption and freeze‑drying processes. Adhesion, expansion and proliferation of BMSCs was investigated in vitro on the C7‑treated β‑TCP scaffolds and compared with pure β‑TCP scaffolds. The results revealed that C7 had a promoting effect on the adhesion, expansion and proliferation of BMSCs on β‑TCP scaffolds. Therefore, C7 may be effective in future tissue engineering therapy for ONFH.
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Copy and paste a formatted citation
Spandidos Publications style
Sun T, Man Z, Peng C, Wang G and Sun S: A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds. Mol Med Rep 20: 1157-1166, 2019.
APA
Sun, T., Man, Z., Peng, C., Wang, G., & Sun, S. (2019). A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds. Molecular Medicine Reports, 20, 1157-1166. https://doi.org/10.3892/mmr.2019.10335
MLA
Sun, T., Man, Z., Peng, C., Wang, G., Sun, S."A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds". Molecular Medicine Reports 20.2 (2019): 1157-1166.
Chicago
Sun, T., Man, Z., Peng, C., Wang, G., Sun, S."A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds". Molecular Medicine Reports 20, no. 2 (2019): 1157-1166. https://doi.org/10.3892/mmr.2019.10335
Copy and paste a formatted citation
x
Spandidos Publications style
Sun T, Man Z, Peng C, Wang G and Sun S: A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds. Mol Med Rep 20: 1157-1166, 2019.
APA
Sun, T., Man, Z., Peng, C., Wang, G., & Sun, S. (2019). A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds. Molecular Medicine Reports, 20, 1157-1166. https://doi.org/10.3892/mmr.2019.10335
MLA
Sun, T., Man, Z., Peng, C., Wang, G., Sun, S."A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds". Molecular Medicine Reports 20.2 (2019): 1157-1166.
Chicago
Sun, T., Man, Z., Peng, C., Wang, G., Sun, S."A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds". Molecular Medicine Reports 20, no. 2 (2019): 1157-1166. https://doi.org/10.3892/mmr.2019.10335
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