Development of an enzyme‑linked immunosorbent assay for camptothecin

Yan,Liting; Nan,Xiang; Zhang,Cunzheng; Wang,Haifang; Huang,Xiaoyan; Hu,Jun; Liu,Yingqian

doi:10.3892/mmr.2019.10342

Development of an enzyme‑linked immunosorbent assay for camptothecin

Authors:
- Liting Yan
- Xiang Nan
- Cunzheng Zhang
- Haifang Wang
- Xiaoyan Huang
- Jun Hu
- Yingqian Liu
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Affiliations: Central Laboratory, Shaanxi Provincial People's Hospital, Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi 710068, P.R. China, School of Pharmacy, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Institute of Food Quality and Safety Inspection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, P.R. China, Institute of Integrated Medicine, Shaanxi University of Traditional Chinese Medicine, Xi'an, Shaanxi 712046, P.R. China
Published online on: June 5, 2019 https://doi.org/10.3892/mmr.2019.10342
Pages: 959-966
Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The use of camptothecin and its analogues has increased in clinical settings and in agriculture. Therefore, camptothecins and their derivatives, metabolites and degradation products are frequently found in the environment. Therefore, it is important to develop an ELISA for the quantification of camptothecins in human plasma, plants, animal tissues and other matrices. The present study developed a novel competitive indirect ELISA for camptothecin using a monoclonal antibody (MAb). In total, two haptens and various carrier proteins were tested to select the most suitable immunogen for the production of MAbs against camptothecin. Hapten 1 conjugated with keyhole limpet hemocyanin was selected for the preparation of MAb 5A3, and was used to establish a competitive indirect ELISA for camptothecin. A total of three derivatives of camptothecin used in clinical practice were examined. Topotecan showed an IC50 value of 0.68 µg/ml with a detection limit of 0.19 µg/ml, belotecan showed an IC50 value of 0.87 µg/ml with a detection limit of 0.22 µg/ml and irinotecan showed an IC50 value of 2.85 µg/ml with a detection limit of 0.47 µg/ml. The cross‑reactivity results suggested that the assay developed in the present study possessed a high sensitivity to camptothecin. Therefore, this immunoassay technique may be suitable for monitoring the levels of camptothecin in compound analysis, clinical applications, and analyses of food and environmental samples.

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