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Preparation of an acellular spinal cord scaffold to improve its biological properties

  • Authors:
    • Hui Xing
    • Hong Yin
    • Chao Sun
    • Xianjun Ren
    • Yongyang Tian
    • Miao Yu
    • Tao Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Army Medical University, Chongqing 400037, P.R. China, Emergency Department of University‑Town Hospital of Chongqing Medical University, Chongqing 401331, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1075-1084
    |
    Published online on: June 6, 2019
       https://doi.org/10.3892/mmr.2019.10364
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Abstract

In recent years, acellular spinal cord scaffolds have been extensively studied in tissue engineering. Notably, acellular spinal cord scaffolds may be used to treat spinal cord injury; however, the method of preparation can result in low efficiency and may affect the biological properties of cells. This study aimed to use EDC crosslinking, combined with chemical extraction for tissue decellularization, in order to improve the efficiency of acellular scaffolds. To make the improved stent available for the clinical treatment of spinal cord injury, it is necessary to study its immunogenicity. Therefore, this study also focused on the adherence of rat bone marrow mesenchymal stem cells to scaffolds, and their differentiation into neuron‑like cells in the presence of suitable trophic factors. The results revealed that EDC crosslinking combined with chemical extraction methods may significantly improve the efficiency of acellular scaffolds, and may also confer better biological characteristics, including improved immunogenicity. Notably, it was able to promote adhesion of rat bone marrow mesenchymal stem cells and their differentiation into neuron‑like cells. These results suggested that the improved preparation method may be promising for the construction of multifunctional acellular scaffolds for the treatment of spinal cord injury.
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1 

Baiguera S, Jungebluth P, Burns A, Mavilia C, Haag J, De Coppi P and Macchiarini P: Tissue engineered human tracheas for in vivo implantation. Biomaterials. 31:8931–8938. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Chen RN, Ho HO, Tsai YT and Sheu MT: Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials. 25:2679–2686. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Ji R, Zhang N, You N, Li Q, Liu W, Jiang N, Liu J, Zhang H, Wang D, Tao K and Dou K: The differentiation of MSCs into functional hepatocyte-like cells in a liver biomatrix scaffold and their transplantation into liver-fibrotic mice. Biomaterials. 35:8995–9008

4 

Woods T and Gratzer PF: Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament-bone graft. Biomaterials. 36:7339–7349. 2005. View Article : Google Scholar

5 

Sánchez PL, Fernández-Santos ME, Costanza S, Climent AM, Moscoso I, Gonzalez-Nicolas MA, Sanz-Ruiz R, Rodríguez H, Kren SM, Garrido G, et al: Acellular human heart matrix: A critical step toward whole heart grafts. Biomaterials. 61:279–289. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Laronda MM, Jakus AE, Whelan KA, Wertheim JA, Shah RN and Woodruff TK: Initiation of puberty in mice following decellularized ovary transplant. Biomaterials. 50:20–29. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Ravindran S, Kotecha M, Huang C, Ye A, Pothirajan P, Yin Z, Magin R and George A: Biological and MRI characterization of biomimetic ECM scaffolds for cartilage tissue regeneration. Biomaterials. 78:58–70. 2015. View Article : Google Scholar

8 

Cai M, Huang T, Hou B and Guo Y: Role of demyelination efficiency within acellular nerve scaffolds during nerve regeneration across peripheral defects. Biomed Res Int. 2017:46063872017. View Article : Google Scholar : PubMed/NCBI

9 

Crapo PM, Medberry CJ, Reing JE, Tottey S, van der Merwe Y, Jones KE and Badylak SF: Biologic scaffolds composed of central nervous system. Biomaterials. 33:3539–3547. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Guo SZ, Ren XJ, Wu B and Jiang T: Preparation of the acellular scaffold of the spinal cord and the study of biocompatibility. Spinal Cord. 48:576–581. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Jiang T, Ren XJ, Tang JL, Yin H, Wang KJ and Zhou CL: Preparation and characterization of genipin-crosslinked rat acellular spinal cord scaffolds. Mater Sci Eng C Mater Biol Appl. 33:3514–3521. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Wang L, Wang ZH, Shen CY, You ML, Xiao JF and Chen GO: Differentiation of human bone marrow mesenchymal stem cells grown in terpolyesters of 3-hydroxyalkanoates scaffolds into nerve cells. Biomaterials. 31:1691–1698. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Zhang K, Liu Z, Li G, Lai BQ, Qin LN, Ding Y, Ruan JW, Zhang SX and Zeng YS: Electro-acupuncture promotes the survival and differentiation of transplanted bone marrow mesenchymal stem cells pre-induced with neurotrophin-3 and retinoic acid in gelatin sponge scaffold after rat spinal cord transection. Stem Cell Rev. 10:612–625. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Guan M, Xu Y, Wang W and Lin S: Differentiation into neurons of rat bone marrow-derived mesenchymal stem cells. Eur Cytokine Netw. 25:58–63. 2014.PubMed/NCBI

15 

Parekkadan B and Milwid JM: Mesenchymal stem cells as therapeutics. Annu Rev Biomed Eng. 12:87–117. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Banks JM, Mozdzen LC, Harley BA and Bailey RC: The combined effects of matrix stiffness and growth factor immobilization on the bioactivity and differentiation capabilities of adipose-derived stem cells. Biomaterials. 35:8951–8959. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Dado D and Levenberg S: Cell-scaffold mechanical interplay within engineered tissue. Semin Cell Deve Biol. 20:656–664. 2009. View Article : Google Scholar

19 

Kehoe S, Zhang XF and Boyd D: FDA approved guidance conduits and wraps for peripheral nerve injury: A review of materials and efficacy. Injury. 45:553–572. 2012. View Article : Google Scholar

20 

Liu J, Chen J, Liu B, Yang C, Xie D, Zheng X, Xu S, Chen T, Wang L, Zhang Z, et al: Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats. J Neurol Sci. 325:127–136. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Chen J, Zhang Z, Liu J, Zhou R, Zheng X, Chen T, Wang L, Huang M, Yang C, Li Z, et al: Acellular spinal cord scaffold seeded with bone marrow stromal cells protects tissue and promotes functional recovery in spinal cord-injured rats. J Neurosci Res. 92:307–317. 2014. View Article : Google Scholar : PubMed/NCBI

22 

See EY, Toh SL and Goh JC: Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: Implications for tissue engineering. Tissue Eng Part A. 16:1421–1431. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Ngangan AV and McDevitt TC: Acellularization of embryoid bodies via physical disruption methods. Biomaterials. 30:1143–1149. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Burk J, Erbe I, Berner D, Kacza J, Kasper C, Pfeiffer B, Winter K and Brehm W: Freeze-thaw cycles enhance decellularization of large tendons. Tissue Eng Part C Methods. 20:276–284. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Grauss RW, Hazekamp MG, Oppenhuizen F, van Munsteren CJ, Gittenberger-de Groot AC and DeRuiter MC: Histological evaluation of decellularised porcine aortic valves: Matrix changes due to different decellularisation methods. Eur J CardioThoracic Surg. 27:566–571. 2005. View Article : Google Scholar

26 

Huang GP, Shanmugasundaram S, Masih P, Pandya D, Amara S, Collins G and Arinzeh TL: An investigation of common crosslinking agents on the stability of electrospun collagen scaffolds. J Biomed Mater Res A. 103:762–771. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Kozowska J and Sionkowska A: Effects of different crosslinking methods on the properties of collagen-calcium phosphate composite materials. Int J Biol Macromol. 74:397–403. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Priyadarshani P, Li Y, Yang S and Yao L: Injectable hydrogel provides growth-permissive environment for human nucleus pulposus cells. J Biomed Mater Res A. 104:419–426. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Wang Y, Wang X, Shi J, Zhu R, Zhang J, Zhang Z, Ma D, Hou Y, Lin F, Yang J and Mizuno M: A biomimetic silk fibroin/sodium alginate composite scaffold for soft tissue engineering. Sci Rep. 6:394772016. View Article : Google Scholar : PubMed/NCBI

30 

Satake K, Lou J and Lenke LG: Migration of mesenchymal stem cells through cerebrospinal fluid into injured spinal cord tissue. Spine (Phila Pa 1976). 29:1971–1979. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Khalatbary AR and Tiraihi T: Localization of bone marrow stromal cells in injured spinal cord treated by intravenous route depends on the hemorrhagic lesions in traumatized spinal tissues. Neurol Res. 29:21–26. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Liu T, Xu J, Chan BP and Chew SY: Sustained release of neurotrophin-3 and chondroitinase ABC from electrospun collagen nanofiber scaffold for spinal cord injury repair. J Biomed Mater Res A. 100:236–242. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Haugh MG, Murphy CM, McKiernan RC, Altenbuchner C and O'Brien FJ: Crosslinking and mechanical properties significantly influence cell attachment, proliferation, and migration within collagen glycosaminoglycan scaffolds. Tissue Eng Part A. 17:1201–1208. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Thitiset T, Damrongsakkul S, Bunaprasert T, Leeanansaksiri W and Honsawek S: Development of collagen/demineralized bone powder scaffolds and periosteum-derived cells for bone tissue engineering application. Int J Mol Sci. 14:2056–2071. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Liu C, McKenna FM, Liang H, Johnstone A and Abel EW: Enhanced cell colonization of collagen scaffold by ultraviolet/ozone surface processing. Tissue Eng Part C Methods. 16:1305–1314. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xing H, Yin H, Sun C, Ren X, Tian Y, Yu M and Jiang T: Preparation of an acellular spinal cord scaffold to improve its biological properties. Mol Med Rep 20: 1075-1084, 2019.
APA
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., & Jiang, T. (2019). Preparation of an acellular spinal cord scaffold to improve its biological properties. Molecular Medicine Reports, 20, 1075-1084. https://doi.org/10.3892/mmr.2019.10364
MLA
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., Jiang, T."Preparation of an acellular spinal cord scaffold to improve its biological properties". Molecular Medicine Reports 20.2 (2019): 1075-1084.
Chicago
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., Jiang, T."Preparation of an acellular spinal cord scaffold to improve its biological properties". Molecular Medicine Reports 20, no. 2 (2019): 1075-1084. https://doi.org/10.3892/mmr.2019.10364
Copy and paste a formatted citation
x
Spandidos Publications style
Xing H, Yin H, Sun C, Ren X, Tian Y, Yu M and Jiang T: Preparation of an acellular spinal cord scaffold to improve its biological properties. Mol Med Rep 20: 1075-1084, 2019.
APA
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., & Jiang, T. (2019). Preparation of an acellular spinal cord scaffold to improve its biological properties. Molecular Medicine Reports, 20, 1075-1084. https://doi.org/10.3892/mmr.2019.10364
MLA
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., Jiang, T."Preparation of an acellular spinal cord scaffold to improve its biological properties". Molecular Medicine Reports 20.2 (2019): 1075-1084.
Chicago
Xing, H., Yin, H., Sun, C., Ren, X., Tian, Y., Yu, M., Jiang, T."Preparation of an acellular spinal cord scaffold to improve its biological properties". Molecular Medicine Reports 20, no. 2 (2019): 1075-1084. https://doi.org/10.3892/mmr.2019.10364
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