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Article Open Access

Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms

Retraction in: /10.3892/mmr.2024.13271
  • Authors:
    • Zhang Sheng Xiong
    • Song Feng Gong
    • Wen Si
    • Taipeng Jiang
    • Qing Long Li
    • Tie Jun Wang
    • Wen Jie Wang
    • Rui Yue Wu
    • Kun Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Longhua District Central Hospital of Shenzhen, Shenzhen, Guangdong 518110, P.R. China, Department of Neurosurgery, The Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518000, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 887-894
    |
    Published online on: June 6, 2019
       https://doi.org/10.3892/mmr.2019.10369
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Abstract

The purpose of the present study was to determine the effects of metformin on the inhibition of proliferation, apoptosis, invasion and migration of A172 human glioma cells in vitro and determine the underlying mechanism. The effects of metformin at different concentrations (0, 0.1, 1 and 10 mmol/l) on the inhibition of A172 cell proliferation were detected using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. Cell apoptosis was detected by flow cytometry. Caspase‑3 activity was analyzed by spectrophotometry. The invasion and migration of cells were detected by Transwell assays. The levels of Bcl‑2‑associated X protein (Bax), B‑cell lymphoma 2 (Bcl‑2), AMP‑activated protein kinase (AMPK), phosphorylated‑(p)AMPK and mechanistic target of rapamycin (mTOR) protein expression were detected by western blot analysis, and changes in the malondialdehyde (MDA) content and activity of superoxide dismutase (SOD) were determined. Compared with the control group, metformin significantly increased the inhibition of proliferation and apoptosis, and significantly reduced the invasion and migration of A172 cells in dose‑ and time‑dependent manners (P<0.05). In addition, compared with the control group, metformin significantly enhanced the activity of caspase‑3, increased the expression of AMPK/pAMPK/Bax proteins and reduced the expression of mTOR/Bcl‑2 proteins (P<0.05). Metformin increased the MDA content and reduced the activity of SOD in a dose‑dependent manner (P<0.05). Metformin may inhibit glioma cell proliferation, migration and invasion, and promote its apoptosis; the effects may be associated with the AMPK/mTOR signaling pathway and oxidative stress.
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View References

1 

Ostrom QT, Gittleman H, Stetson L, Virk SM and Barnholtz-Sloan JS: Epidemiology of gliomas. Cancer Treat Res. 163:1–14. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Farmer RE, Ford D, Forbes HJ, Chaturvedi N, Kaplan R, Smeeth L and Bhaskaran K: Metformin and cancer in type 2 diabetes: A systematic review and comprehensive bias evaluation. Int J Epidemiol. 46:7452017. View Article : Google Scholar : PubMed/NCBI

3 

Li C, Xue Y, Xi YR and Xie K: Progress in the application and mechanism of metformin in treating non-small cell lung cancer. Oncol Lett. 13:2873–2880. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Yousef M and Tsiani E: Metformin in lung cancer: Review of in vitro and in vivo animal studies. Cancers (Basel). 9(pii): E452017. View Article : Google Scholar : PubMed/NCBI

5 

Whitburn J, Edwards CM and Sooriakumaran P: Metformin and prostate cancer: A new role for an old drug. Curr Urol Rep. 18:462017. View Article : Google Scholar : PubMed/NCBI

6 

Rêgo DF, Elias ST, Amato AA, Canto GL and Guerra EN: Anti-tumor effects of metformin on head and neck carcinoma cell lines: A systematic review. Oncol Lett. 13:554–566. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Zhou PT, Li B, Liu FR, Zhang MC, Wang Q, Li YY, Xu C, Liu YH, Yao Y and Li D: Metformin is associated with surival benefit in pancreatic cancer patients with diabetes: A systematic review and meta-analysis. Oncotarget. 8:25242–25250. 2017.PubMed/NCBI

8 

Hankinson SJ, Fam M and Patel NN: A review for clinicians: Prostate cancer and the antineoplastic properties of metformin. Urol Oncol. 35:21–29. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Perez-Lopez FR, Pasupuleti V, Gianuzzi X, Palma-Ardiles G, Hernandez-Fernandez W and Hernandez AV: Systematic review and meta-analysis of the effect of metformin treatment on overall mortality rates in women with endometrial cancer and type 2 diabetes mellitus. Maturitas. 101:6–11. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Samsuri NAB, Leech M and Marignol L: Metformin and improved treatment outcomes in radiation therapy-A review. Cancer Treat Rev. 55:150–162. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Zhou PT, Li B, Liu FR, Zhang MC, Wang Q, Li YY, Xu C, Liu YH, Yao Y and Li D: Metformin is associated with survival benefit in pancreatic cancer patients withdiabetes: A systematic review and meta-analysis. Oncotarget. 8:25242–25250. 2017.PubMed/NCBI

12 

Yu Z, Zhao G, Xie G, Zhao L, Chen Y, Yu H, Zhang Z, Li C and Li Y: Metformin and temozolomide act synergistically to inhibit growth of glioma cells and glioma stem cells in vitro and in vivo. Oncotarget. 6:32930–32943. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Seliger C, Meyer AL, Renner K, Leidgens V, Moeckel S, Jachnik B, Dettmer K, Tischler U, Gerthofer V, Rauer L, et al: Metformin inhibits proliferation and migration of glioblastoma cells independently of TGF-β2. Cell Cycle. 15:1755–1766. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Seliger C, Ricci C, Meier CR, Bodmer M, Jick SS, Bogdahn U, Hau P and Leitzmann MF: Diabetes, use of antidiabetic drugs, and the risk of glioma. Neuro Oncol. 18:340–349. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Guigas B and Viollet B: Targeting AMPK: From ancient drugs to new small-molecule activators. Exp Suppl. 107:327–350. 2016.PubMed/NCBI

16 

Cheng J, Zhang T, Ji H, Tao K, Guo J and Wei W: Functional characterization of AMP-activated protein kinase signaling in tumorigenesis. Biochim Biophys Acta. 1866:232–251. 2016.PubMed/NCBI

17 

Stopsack KH, Greenberg AJ and Mucci LA: Common medications and prostate cancer mortality: A review. World J Urol. 35:875–882. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Yang SH, Li S, Lu G, Xue H, Kim DH, Zhu JJ and Liu Y: Metformin treatment reduces temozolomide resistance of glioblastoma cells. Oncotarget. 7:78787–78803. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Liu X, Chhipa RR, Pooya S, Wortman M, Yachyshin S, Chow LM, Kumar A, Zhou X, Sun Y, Quinn B, et al: Discrete mechanisms of mTOR and cell cycle regulation by AMPK agonists independent of AMPK. Proc Natl Acad Sci USA. 111:E435–E444. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Carmignani M, Volpe AR, Aldea M, Soritau O, Irimie A, Florian IS, Tomuleasa C, Baritchii A, Petrushev B, Crisan G and Valle G: Glioblastoma stem cells: A new target for metformin and arsenic trioxide. J Biol Regul Homeost Agents. 28:1–15. 2014.PubMed/NCBI

21 

Podhorecka M, Ibanez B and Dmoszyńska A: Metformin-its potential anti-cancer and anti-aging effects. Postepy Hig Med Dosw (Online). 71:170–175. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Du L, Wang M, Kang Y, Li B, Guo M, Cheng Z and Bi C: Prognostic role of metformin intake in diabetic patients with colorectal cancer: An updated qualitative evidence of cohort studies. Oncotarget. 8:26448–26459. 2017.PubMed/NCBI

23 

Zhao B, Wang X, Zheng J, Wang H and Liu J: Effects of metformin treatment on glioma-induced brain edema. Am J Transl Res. 8:3351–3363. 2016.PubMed/NCBI

24 

Liu F, Yan L, Wang Z, Lu Y, Chu Y, Li X, Liu Y, Rui D, Nie S and Xiang H: Metformin therapy and risk of colorectal adenomas and colorectal cancer in type 2 diabetes mellitus patients: A systematic review and meta-analysis. Oncotarget. 8:16017–16026. 2017.PubMed/NCBI

25 

Rukoyatkina N, Butt E, Subramanian H, Nikolaev VO, Mindukshev I, Walter U, Gambaryan S and Benz PM: Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis. Cell Death Dis. 8:e28982017. View Article : Google Scholar : PubMed/NCBI

26 

Li H, Chen X, Yu Y, Wang Z, Zuo Y, Li S, Yang D, Hu S, Xiang M, Xu Z and Yu Z: Metformin inhibits the growth of nasopharyngeal carcinoma cells and sensitizes the cells to radiation via inhibition of the DNA damage repair pathway. Oncol Rep. 32:2596–2604. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Leidgens V, Proske J, Rauer L, Moeckel S, Renner K, Bogdahn U, Riemenschneider MJ, Proescholdt M, Vollmann-Zwerenz A, Hau P and Seliger C: Stattic and metformin inhibit brain tumor initiating cells by reducing STAT3-phosphorylation. Oncotarget. 8:8250–8263. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Pantovic A, Bosnjak M, Arsikin K, Kosic M, Mandic M, Ristic B, Tosic J, Grujicic D, Isakovic A, Micic N, et al: In vitro antiglioma action of indomethacin is mediated via AMP-activated protein kinase/mTOR complex 1 signalling pathway. Int J Biochem Cell Biol. 83:84–96. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Sesen J, Dahan P, Scotland SJ, Saland E, Dang VT, Lemarié A, Tyler BM, Brem H, Toulas C, Cohen-Jonathan Moyal E, et al: Metformin inhibits growth of human glioblastoma cells and enhances therapeutic response. PLoS One. 10:e1237212015. View Article : Google Scholar

30 

Hall J, Prabhakar S, Balaj L, Lai CP, Cerione RA and Breakefield XO: Delivery of therapeutic proteins via extracellular vesicles: Review and potential treatments for Parkinson's disease, glioma, and schwannoma. Cell Mol Neurobiol. 36:417–427. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xiong ZS, Gong SF, Si W, Jiang T, Li QL, Wang TJ, Wang WJ, Wu RY and Jiang K: Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271. Mol Med Rep 20: 887-894, 2019.
APA
Xiong, Z.S., Gong, S.F., Si, W., Jiang, T., Li, Q.L., Wang, T.J. ... Jiang, K. (2019). Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271. Molecular Medicine Reports, 20, 887-894. https://doi.org/10.3892/mmr.2019.10369
MLA
Xiong, Z. S., Gong, S. F., Si, W., Jiang, T., Li, Q. L., Wang, T. J., Wang, W. J., Wu, R. Y., Jiang, K."Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271". Molecular Medicine Reports 20.2 (2019): 887-894.
Chicago
Xiong, Z. S., Gong, S. F., Si, W., Jiang, T., Li, Q. L., Wang, T. J., Wang, W. J., Wu, R. Y., Jiang, K."Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271". Molecular Medicine Reports 20, no. 2 (2019): 887-894. https://doi.org/10.3892/mmr.2019.10369
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong ZS, Gong SF, Si W, Jiang T, Li QL, Wang TJ, Wang WJ, Wu RY and Jiang K: Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271. Mol Med Rep 20: 887-894, 2019.
APA
Xiong, Z.S., Gong, S.F., Si, W., Jiang, T., Li, Q.L., Wang, T.J. ... Jiang, K. (2019). Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271. Molecular Medicine Reports, 20, 887-894. https://doi.org/10.3892/mmr.2019.10369
MLA
Xiong, Z. S., Gong, S. F., Si, W., Jiang, T., Li, Q. L., Wang, T. J., Wang, W. J., Wu, R. Y., Jiang, K."Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271". Molecular Medicine Reports 20.2 (2019): 887-894.
Chicago
Xiong, Z. S., Gong, S. F., Si, W., Jiang, T., Li, Q. L., Wang, T. J., Wang, W. J., Wu, R. Y., Jiang, K."Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms Retraction in /10.3892/mmr.2024.13271". Molecular Medicine Reports 20, no. 2 (2019): 887-894. https://doi.org/10.3892/mmr.2019.10369
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