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Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling

  • Authors:
    • Jianhui Li
    • Junjun Jia
    • Ning He
    • Li Jiang
    • Hao Yu
    • Haoyu Li
    • Yifan Peng
    • Haiyang Xie
    • Lin Zhou
    • Shusen Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1663-1671
    |
    Published online on: June 12, 2019
       https://doi.org/10.3892/mmr.2019.10379
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Abstract

A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 12 rats were randomly assigned into two groups (n=6 for each group): The kidney‑liver perfusion (KL) group and liver perfusion (LP) group. Perfusate samples were collected during the perfusion process for the analysis of pH, K+ and liver function. Liver tissues were obtained for the evaluation of adenosine triphosphate (ATP), terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling and immunohistochemistry of Ki67. Cell cycle inhibitors, apoptosis‑associated genes and signal transducer and activator of transcription 3 (Stat3) were analyzed using quantitative polymerase chain reaction and western blot analysis. Results: Overall pH and K+ values of the KL group were significantly different from the LP group and more stable; aspartate aminotransferase, alanine transaminase and lactate dehydrogenase levels increased progressively over time in the LP group and were significantly different at different time points compared with pre‑perfusion levels and the KL group, which suggested the KL group was superior to the LP group. In addition, KL reduced portal vein resistance and was associated with lower ATP consumption compared with the LP group. Furthermore, liver proliferation was upregulated with the upregulation of the interleukin 6 (IL‑6)/Stat3 signaling pathway in KL compared with LP. The present study revealed for the first time that KL and hypothermic machine perfusion demonstrated a more proactive repair capability by maintaining liver regeneration via the upregulation of the IL‑6/Stat3 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Jia J, He N, Jiang L, Yu H, Li H, Peng Y, Xie H, Zhou L, Zheng S, Zheng S, et al: Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling. Mol Med Rep 20: 1663-1671, 2019.
APA
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H. ... Zheng, S. (2019). Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling. Molecular Medicine Reports, 20, 1663-1671. https://doi.org/10.3892/mmr.2019.10379
MLA
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H., Peng, Y., Xie, H., Zhou, L., Zheng, S."Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling". Molecular Medicine Reports 20.2 (2019): 1663-1671.
Chicago
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H., Peng, Y., Xie, H., Zhou, L., Zheng, S."Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling". Molecular Medicine Reports 20, no. 2 (2019): 1663-1671. https://doi.org/10.3892/mmr.2019.10379
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Jia J, He N, Jiang L, Yu H, Li H, Peng Y, Xie H, Zhou L, Zheng S, Zheng S, et al: Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling. Mol Med Rep 20: 1663-1671, 2019.
APA
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H. ... Zheng, S. (2019). Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling. Molecular Medicine Reports, 20, 1663-1671. https://doi.org/10.3892/mmr.2019.10379
MLA
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H., Peng, Y., Xie, H., Zhou, L., Zheng, S."Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling". Molecular Medicine Reports 20.2 (2019): 1663-1671.
Chicago
Li, J., Jia, J., He, N., Jiang, L., Yu, H., Li, H., Peng, Y., Xie, H., Zhou, L., Zheng, S."Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling". Molecular Medicine Reports 20, no. 2 (2019): 1663-1671. https://doi.org/10.3892/mmr.2019.10379
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