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Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3

  • Authors:
    • Meng Zhou
    • Xiaoqian Yu
    • Zhenhai Jing
    • Wei Wu
    • Chenglong Lu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Qilu Hospital of Shandong University, Qingdao, Shandong 266000, P.R. China, Department of Dermatology, Qingdao Hiser Medical Group, Qingdao Hospital of Traditional Chinese Medicine, Qingdao, Shandong 266032, P.R. China, Department of Oncology, Qingdao Hiser Medical Group, Qingdao Hospital of Traditional Chinese Medicine, Qingdao, Shandong 266032, P.R. China, College of Food Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266179, P.R. China, Department of Emergency, Qilu Hospital of Shandong University, Qingdao, Shandong 266000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1797-1807
    |
    Published online on: June 20, 2019
       https://doi.org/10.3892/mmr.2019.10408
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Abstract

Melanoma is an aggressive skin carcinoma with poor prognosis, and is prevalent worldwide. It was demonstrated that microRNA (miR)‑21 and mitogen‑activated protein kinase kinase 3 (MKK3) both participated in the occurrence and development of various tumors; however, their detailed roles in the progression of melanoma remain unclear. Reverse transcription‑quantitative PCR (RT‑qPCR) and western blot analyses were conducted to examine the expression levels of miR‑21 and MKK3 in clinical specimens of patients with melanoma and melanoma cell lines. A dual‑luciferase reporter assay was performed to verify the target interaction between miR‑21 and MKK3. The mRNA and protein expressions of MKK3 were measured using RT‑qPCR and western blot analysis, respectively, following transfection with miR‑21 mimics and inhibitor. Subsequently, Cell Counting Kit‑8 and colony formation assays, and flow cytometry were conducted to assess the effects of miR‑21 and MKK3 on the cell growth of melanoma. Cell migration and invasion experiments were performed to evaluate the effects of miR‑21 and MKK3 on the cell metastasis of melanoma. It was revealed that MKK3 was upregulated, and miR‑21 was downregulated in patients with melanoma and melanoma cell lines. MKK3 was demonstrated to be a direct target of miR‑21. Furthermore, it was demonstrated that upregulated miR‑21 expression and downregulated MKK3 expression suppressed cell proliferation and colony formation, promoted apoptosis, delayed the cell cycle, and inhibited cell migration and invasion. The present findings suggested that miR‑21 could inhibit the cell growth and metastasis of melanoma by negatively regulating MKK3.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou M, Yu X, Jing Z, Wu W and Lu C: Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3. Mol Med Rep 20: 1797-1807, 2019.
APA
Zhou, M., Yu, X., Jing, Z., Wu, W., & Lu, C. (2019). Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3. Molecular Medicine Reports, 20, 1797-1807. https://doi.org/10.3892/mmr.2019.10408
MLA
Zhou, M., Yu, X., Jing, Z., Wu, W., Lu, C."Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3". Molecular Medicine Reports 20.2 (2019): 1797-1807.
Chicago
Zhou, M., Yu, X., Jing, Z., Wu, W., Lu, C."Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3". Molecular Medicine Reports 20, no. 2 (2019): 1797-1807. https://doi.org/10.3892/mmr.2019.10408
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou M, Yu X, Jing Z, Wu W and Lu C: Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3. Mol Med Rep 20: 1797-1807, 2019.
APA
Zhou, M., Yu, X., Jing, Z., Wu, W., & Lu, C. (2019). Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3. Molecular Medicine Reports, 20, 1797-1807. https://doi.org/10.3892/mmr.2019.10408
MLA
Zhou, M., Yu, X., Jing, Z., Wu, W., Lu, C."Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3". Molecular Medicine Reports 20.2 (2019): 1797-1807.
Chicago
Zhou, M., Yu, X., Jing, Z., Wu, W., Lu, C."Overexpression of microRNA‑21 inhibits the growth and metastasis of melanoma cells by targeting MKK3". Molecular Medicine Reports 20, no. 2 (2019): 1797-1807. https://doi.org/10.3892/mmr.2019.10408
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