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Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)

  • Authors:
    • Na Lin
    • Xiu‑Juan Dong
    • Ting‑Yong Wang
    • Wen‑Ji He
    • Jing Wei
    • Hai‑Ying Wu
    • Ting‑Hua Wang
  • View Affiliations / Copyright

    Affiliations: Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan 650500, P.R. China, Department of Physical Education, Hainan Normal University, Haikou, Hainan 571100, P.R. China, Department of Ultrasonic Cardiogram, Kunming Children's Hospital, Kunming Medical University, Kunming, Yunnan 650228, P.R. China, Department of Pharmacy, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000, P.R. China, Department of Emergency, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1819-1825
    |
    Published online on: June 25, 2019
       https://doi.org/10.3892/mmr.2019.10422
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Abstract

Tree shrews are most closely related to the primates and so possess a number of advantages in experimental studies; they have been used as an animal model in bacterial and virus infection, cancer, endocrine system disease, and certain nervous system diseases. Their olfactory ensheathing cells (OECs) are able to release several cytokines to promote neuronal survival, regeneration and remyelination. The present study used western blot analysis to identify antibody specificity in protein extracts from whole tree shrew brains to identify the specificity of p75 nerve growth factor receptor (NGFR) derived from rabbits (75 kDa). OECs were cultured and isolated, then stained and identified using the antibodies for p75NGFR. To investigate the capacity of OECs to express cytokines and growth factors, microarray technology was used, and the analysis revealed that OECs were able to express 9,821 genes. Of these genes, 44 genes were from the neurotrophic factor family, which may indicate their potential in transplantation in vivo. The present study considered the function of OECs as revealed by other studies, and may contribute to future research.
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Copy and paste a formatted citation
Spandidos Publications style
Lin N, Dong XJ, Wang TY, He WJ, Wei J, Wu HY and Wang TH: Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep 20: 1819-1825, 2019.
APA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., & Wang, T. (2019). Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Molecular Medicine Reports, 20, 1819-1825. https://doi.org/10.3892/mmr.2019.10422
MLA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20.2 (2019): 1819-1825.
Chicago
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20, no. 2 (2019): 1819-1825. https://doi.org/10.3892/mmr.2019.10422
Copy and paste a formatted citation
x
Spandidos Publications style
Lin N, Dong XJ, Wang TY, He WJ, Wei J, Wu HY and Wang TH: Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep 20: 1819-1825, 2019.
APA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., & Wang, T. (2019). Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Molecular Medicine Reports, 20, 1819-1825. https://doi.org/10.3892/mmr.2019.10422
MLA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20.2 (2019): 1819-1825.
Chicago
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20, no. 2 (2019): 1819-1825. https://doi.org/10.3892/mmr.2019.10422
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