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Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells

  • Authors:
    • Fang Ju
    • Na Li
    • Wenming Wang
    • Haicheng Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Qingdao Central Hospital, Qingdao, Shandong 266042, P.R. China, Department of Respiratory Medicine, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China, Department of Neurology, Qingdao Central Hospital, Qingdao, Shandong 266042, P.R. China
    Copyright: © Ju et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2503-2508
    |
    Published online on: January 28, 2019
       https://doi.org/10.3892/mmr.2019.9899
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Abstract

Colorectal cancer (CRC), also known as bowel cancer, is one of the leading causes of cancer‑associated mortality worldwide at present. The aim of the present study was to detect the effects of matrix metalloproteinase 1 (MMP1) on the viability and migration of a CRC cell line in the presence or absence of variation X‑ray radiation doses. The CRC cell line, SW620, was cultured and treated with different X‑ray doses (0, 0.1, 0.5, 1, 3 and 6 Gy). MMP1 expression was downregulated via the application of a specific small interfering (si)‑RNA. The viability and migration of SW620 cells prior to and following transfection were detected with MTT and Transwell chamber assays, respectively. The application of siRNA transfection to silence MMP1 in SW620 cells resulted in reduced cell viability and migration (P<0.05). Compared with the control, the cell viability and migration of cells were significantly reduced when exposed to 0.5, 1, 3, and 6 Gy X‑ray radiation (P<0.05). In SW620 cells treated with different X‑ray doses, the mRNA expression levels of MMP1 were significantly reduced (P<0.05). Cells treated with 0.5 Gy X‑ray exposure exhibited the lowest mRNA expression levels of MMP1 when compared with other doses of X‑ray radiation. The expression of MMP1 was associated with the promotion of the viability and migration of SW620 cells. X‑ray radiation with 6 Gy dosages significantly reduced cell viability when compared with the control. Thus, MMP1‑targeted therapy combined with radiotherapy could be used for treating CRC.
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1 

McGuire S: World cancer report 2014. Geneva, Switzerland: World Health Organization, International Agency for Research on Cancer, WHO press, 2015. Adv Nutr. 7:418–419. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Fearon ER and Vogelstein B: A genetic model for colorectal tumorigenesis. Cell. 61:759–767. 1990. View Article : Google Scholar : PubMed/NCBI

3 

Lange F, Franz B, Maletzki C, Linnebacher M, Hühns M and Jaster R: Biological and molecular effects of small molecule kinase inhibitors on low-passage human colorectal cancer cell lines. Biomed Res Int. 2014:5686932014. View Article : Google Scholar : PubMed/NCBI

4 

Holm T, Cedermark B and Rutqvist LE: Local recurrence of rectal adenocarcinoma after ‘curative’ surgery with and without preoperative radiotherapy. Br J Surg. 81:452–455. 1994. View Article : Google Scholar : PubMed/NCBI

5 

Hanahan D and Weinberg RA: The hallmarks of cancer. Cell. 100:57–70. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Park IJ and Yu CS: Current issues in locally advanced colorectal cancer treated by preoperative chemoradiotherapy. World J Gastroenterol. 20:2023–2029. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Braendengen M, Tveit KM, Berglund A, Birkemeyer E, Frykholm G, Påhlman L, Wiig JN, Byström P, Bujko K and Glimelius B: Randomized phase III study comparing preoperative radiotherapy with chemoradiotherapy in nonresectable rectal cancer. J Clin Oncol. 26:3687–3694. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Kao PS, Chang SC, Wang LW, Lee RC, Liang WY, Lin TC, Chen WS, Jiang JK, Yang SH, Wang HS and Lin JK: The impact of preoperative chemoradiotherapy on advanced low rectal cancer. J Surg Oncol. 102:771–777. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Parsons SL, Watson SA, Brown PD, Collins HM and Steele RJ: Matrix metalloproteinases. Br J Surg. 84:160–166. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Visse R and Nagase H: Matrix metalloproteinases and tissue inhibitors of metalloproteinases: Structure, function, and biochemistry. Circ Res. 92:827–839. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Zucker S and Vacirca J: Role of matrix metalloproteinases (MMPs) in colorectal cancer. Cancer Metastasis Rev. 23:101–117. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Arakaki PA, Marques MR and Santos MC: MMP-1 polymorphism and its relationship to pathological processes. J Biosci. 34:313–320. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Murray GI, Duncan ME, O'Neil P, Melvin WT and Fothergill JE: Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer. Nat Med. 2:461–462. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Zinzindohoué F, Lecomte T, Ferraz JM, Houllier AM, Cugnenc PH, Berger A, Blons H and Laurent-Puig P: Prognostic significance of MMP-1 and MMP-3 functional promoter polymorphisms in colorectal cancer. Clin Cancer Res. 11:594–599. 2005.PubMed/NCBI

15 

Bendardaf R, Buhmeida A, Ristamäki R, Syrjänen K and Pyrhönen S: MMP-1 (collagenase-1) expression in primary colorectal cancer and its metastases. Scand J Gastroenterol. 42:1473–1478. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Han G, Wei Z, Lu Z, Cui H, Bai X, Ge H and Zhang W: Association between matrix metalloproteinase 1 −1607 1G>2G polymorphism and cancer risk: A meta-analysis including 19706 subjects. Int J Clin Exp Med. 7:2992–2999. 2014.PubMed/NCBI

17 

Drzymala M, Hawkins M, Henrys A, Bedford J, Norman A and Tait D: The effect of treatment position, prone or supine, on dose-volume histograms for pelvic radiotherapy in patients with rectal cancer. Br J Radiol. 82:321–327. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Kim JY, Kim DY, Kim TH, Park SY, Lee SB, Shin KH, Pyo H, Kim JY and Cho KH: Intensity-modulated radiotherapy with a belly board for rectal cancer. Int J Colorectal Dis. 22:373–379. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AM and Bos JL: Genetic alterations during colorectal-tumor development. N Engl J Med. 319:525–532. 1988. View Article : Google Scholar : PubMed/NCBI

21 

Häfner MF and Debus J: Radiotherapy for colorectal cancer: Current standards and future perspectives. Visc Med. 32:172–177. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Tahara K, Mimori K, Iinuma H, Iwatsuki M, Yokobori T, Ishii H, Anai H, Kitano S and Mori M: Serum matrix-metalloproteinase-1 is a bona fide prognostic marker for colorectal cancer. Ann Surg Oncol. 17:3362–3369. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Teran BL and Thomas J: Antimycin induces apoptosis in rapamycin resistant SW620 colorectal cancer cells. FASEB J. 27:793–798. 2013.PubMed/NCBI

24 

Westermarck J and Kähäri VM: Regulation of matrix metalloproteinase expression in tumor invasion. FASEB J. 13:781–792. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Langenskiöld M, Ivarsson ML, Holmdahl L, Falk P, Kåbjörn-Gustafsson C and Angenete E: Intestinal mucosal MMP-1-a prognostic factor in colon cancer. Scand J Gastroenterol. 48:563–569. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Nishioka M, Shimada M, Kurita N, Iwata T, Morimoto S, Yoshikawa K, Higashijima J and Miyatani T: Gene expression profile can predict pathological response to preoperative chemoradiotherapy in rectal cancer. Cancer Genomics Proteomics. 8:87–92. 2011.PubMed/NCBI

27 

Artacho-Cordón F, Ríos-Arrabal S, Lara PC, Artacho-Cordón A, Calvente I and Núñez MI: Matrix metalloproteinases: Potential therapy to prevent the development of second malignancies after breast radiotherapy. Surg Oncol. 21:143–151. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Angenete E, Oresland T, Falk P, Breimer M, Hultborn R and Ivarsson ML: Preoperative radiotherapy and extracellular matrix remodeling in rectal mucosa and tumour matrix metalloproteinases and plasminogen components. Acta Oncol. 48:1144–1151. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Supiot S, Gouraud W, Campion LC, Jezéquel P, Buecher B, Charrier J, Heymann MF, Mahé MA, Rio E and Chérel M: Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study. World J Gastroenterol. 19:3249–3254. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Jo S, Choi Y, Park SK, Kim JY, Kim HJ, Lee YH, Oh WY, Cho H and Ahn KJ: Efficacy of dose-escalated radiotherapy for recurrent colorectal cancer. Ann Coloproctol. 32:66–72. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Sanghera P, Wong DW, McConkey CC, Geh JI and Hartley A: Chemoradiotherapy for rectal cancer: An updated analysis of factors affecting pathological response. Clin Oncol (R Coll Radiol). 20:176–183. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Sun K, Zhang X, Deng H, et al: Effects of X-ray dose on expression of microRNA-221 and p57~ (kip2) in human colorectal carcinoma cells. Cancer Res Prev Treat. 40:921–924. 2013.

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Copy and paste a formatted citation
Spandidos Publications style
Ju F, Li N, Wang W and Yuan H: Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells. Mol Med Rep 19: 2503-2508, 2019.
APA
Ju, F., Li, N., Wang, W., & Yuan, H. (2019). Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells. Molecular Medicine Reports, 19, 2503-2508. https://doi.org/10.3892/mmr.2019.9899
MLA
Ju, F., Li, N., Wang, W., Yuan, H."Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells". Molecular Medicine Reports 19.4 (2019): 2503-2508.
Chicago
Ju, F., Li, N., Wang, W., Yuan, H."Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells". Molecular Medicine Reports 19, no. 4 (2019): 2503-2508. https://doi.org/10.3892/mmr.2019.9899
Copy and paste a formatted citation
x
Spandidos Publications style
Ju F, Li N, Wang W and Yuan H: Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells. Mol Med Rep 19: 2503-2508, 2019.
APA
Ju, F., Li, N., Wang, W., & Yuan, H. (2019). Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells. Molecular Medicine Reports, 19, 2503-2508. https://doi.org/10.3892/mmr.2019.9899
MLA
Ju, F., Li, N., Wang, W., Yuan, H."Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells". Molecular Medicine Reports 19.4 (2019): 2503-2508.
Chicago
Ju, F., Li, N., Wang, W., Yuan, H."Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells". Molecular Medicine Reports 19, no. 4 (2019): 2503-2508. https://doi.org/10.3892/mmr.2019.9899
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