Regulation of Fgf15 expression in the intestine by glucocorticoid receptor

Jia,Kunzhi; Zhang,Danping; Jia,Qi; Zhang,Qing‑Yu

doi:10.3892/mmr.2019.9915

Regulation of Fgf15 expression in the intestine by glucocorticoid receptor

Authors:
- Kunzhi Jia
- Danping Zhang
- Qi Jia
- Qing‑Yu Zhang
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Affiliations: The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, School of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education/College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China, Wadsworth Center, New York State Department of Health, School of Public Health, State University of New York, Albany, NY 12201, USA
Published online on: January 30, 2019 https://doi.org/10.3892/mmr.2019.9915
Pages: 2953-2959

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Abstract

Fibroblast growth factor 15 (FGF15) was previously identified to be highly expressed in the ileum and functions as an endocrine factor to regulate bile acid synthesis in the liver. FGF15 targets its receptor fibroblast growth factor receptor 4 in the liver and serves important roles in energy metabolism, including bile acid homeostasis, glucose metabolism and protein synthesis. The expression of FGF15 is known to be regulated by the transcription factor farnesoid X receptor (FXR). In the present study, reverse transcription‑quantitative polymerase chain reaction was used for measuring Fgf15 expression from the animal and tissue culture experiments, and it was identified that dexamethasone, a drug widely used in anti‑inflammation therapy, and a classical inducer of glucocorticoid receptor (GR)‑ and pregnane X receptor (PXR)‑target genes, may downregulate Fgf15 expression in the ileum. GR was identified to be highly expressed in the ileum by western blot analysis. Furthermore, it was demonstrated that the downregulation of Fgf15 by dexamethasone is due to the repression of ileal FXR activity via GR; however, not PXR, in the ileum. The present results provide insight for a better understanding of the adverse effects associated with dexamethasone therapy.

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