Open Access

Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis

  • Authors:
    • Qifan Zhang
    • Shibo Sun
    • Chen Zhu
    • Yujian Zheng
    • Qing Cai
    • Xiaolu Liang
    • Haorong Xie
    • Jie Zhou
  • View Affiliations

  • Published online on: February 4, 2019     https://doi.org/10.3892/mmr.2019.9929
  • Pages: 2479-2488
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify the differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor tissue of hepatocellular carcinoma (HCC) samples in order to investigate the mechanisms of HCC. The microarray data of the datasets GSE76427, GSE84005 and GSE57957 were downloaded from the Gene Expression Omnibus database. DEGs were identified using the limma package in the R programming language. Following the intersection of the DEGs screened from the three datasets, 218 genes were selected for further study. A protein‑protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes database. The construction and analysis of modules were performed using Cytoscape and the module with the highest score was selected for further analysis. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were conducted for genes involved in the PPI network and the selected subnetwork. The network of the enriched pathways and their associated genes was constructed using Cytoscape. For the genes in the global PPI network, metabolism‑associated pathways were significantly enriched; whereas, for the genes in the subnetwork, ‘cell cycle’, ‘oocyte meiosis’ and ‘DNA replication’ pathways were significantly enriched. To demonstrate the portability and repeatability of the prognostic value of the weighted genes, a validation cohort was obtained from datasets of The Cancer Genome Atlas and Kaplan‑Meier survival analysis was conducted. Evidence is presented that the expression levels of aldehyde dehydrogenase 2 family member, cytochrome P450 family 2 subfamily C member 8, alcohol dehydrogenase 4 (class II), pi polypeptide, alcohol dehydrogenase 1B (class I), β polypeptide and cytochrome P450 family 2 subfamily C member 9 were associated with the overall survival of patients with HCC and that the expression levels of pituitary tumor‑transforming 1, cell division cycle 20, DNA topoisomerase II α and cyclin B2 were negatively associated with the overall survival of patients with HCC. In conclusion, 9 weighted genes, involved in the development and progression of HCC, were identified using bioinformatics and survival analyses.
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April-2019
Volume 19 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang Q, Sun S, Zhu C, Zheng Y, Cai Q, Liang X, Xie H and Zhou J: Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis. Mol Med Rep 19: 2479-2488, 2019
APA
Zhang, Q., Sun, S., Zhu, C., Zheng, Y., Cai, Q., Liang, X. ... Zhou, J. (2019). Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis. Molecular Medicine Reports, 19, 2479-2488. https://doi.org/10.3892/mmr.2019.9929
MLA
Zhang, Q., Sun, S., Zhu, C., Zheng, Y., Cai, Q., Liang, X., Xie, H., Zhou, J."Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis". Molecular Medicine Reports 19.4 (2019): 2479-2488.
Chicago
Zhang, Q., Sun, S., Zhu, C., Zheng, Y., Cai, Q., Liang, X., Xie, H., Zhou, J."Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis". Molecular Medicine Reports 19, no. 4 (2019): 2479-2488. https://doi.org/10.3892/mmr.2019.9929