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PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis

  • Authors:
    • Yuanshu Zhao
    • Shoubo Zhang
  • View Affiliations / Copyright

    Affiliations: Functional Experiment Center, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 511436, P.R. China, Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510507, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2497-2502
    |
    Published online on: February 4, 2019
       https://doi.org/10.3892/mmr.2019.9930
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Abstract

Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan‑induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. PGAM1 expression was detected in mouse models of busulfan‑induced hypospermatogenesis by western blotting, reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry. Then, spermatogenic cell apoptosis in mouse models of busulfan‑induced hypospermatogenesis was assessed by TUNEL assay. The effect and potential mechanism of PGAM1 downregulation on spermatogenic cells were further investigated. The results indicated that PGAM1 expression was significantly downregulated in the mouse models of busulfan‑induced hypospermatogenesis, compared with those with normal spermatogenesis (P<0.05). Furthermore, the TUNEL assay revealed that the apoptosis of spermatogenic cells was accelerated in the mouse model of busulfan‑induced hypospermatogenesis. In addition, PGAM1 knockdown promoted the apoptosis of spermatogenic cells in vitro, which was associated with the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway. In conclusion, these data indicate that PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and contributes to spermatogenic cell apoptosis by regulating the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y and Zhang S: PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis. Mol Med Rep 19: 2497-2502, 2019.
APA
Zhao, Y., & Zhang, S. (2019). PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis. Molecular Medicine Reports, 19, 2497-2502. https://doi.org/10.3892/mmr.2019.9930
MLA
Zhao, Y., Zhang, S."PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis". Molecular Medicine Reports 19.4 (2019): 2497-2502.
Chicago
Zhao, Y., Zhang, S."PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis". Molecular Medicine Reports 19, no. 4 (2019): 2497-2502. https://doi.org/10.3892/mmr.2019.9930
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y and Zhang S: PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis. Mol Med Rep 19: 2497-2502, 2019.
APA
Zhao, Y., & Zhang, S. (2019). PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis. Molecular Medicine Reports, 19, 2497-2502. https://doi.org/10.3892/mmr.2019.9930
MLA
Zhao, Y., Zhang, S."PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis". Molecular Medicine Reports 19.4 (2019): 2497-2502.
Chicago
Zhao, Y., Zhang, S."PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis". Molecular Medicine Reports 19, no. 4 (2019): 2497-2502. https://doi.org/10.3892/mmr.2019.9930
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