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Article

MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma

  • Authors:
    • Liping Guo
    • Baoli Li
    • Meijing Miao
    • Jianjun Yang
    • Jinshan Ji
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Medical College of Yan'an University, Yan'an, Shanxi 716000, P.R. China, Department of Pharmacology, Medical College of Yan'an University, Yan'an, Shanxi 716000, P.R. China, Department of Nursing, Medical College of Yan'an University, Yan'an, Shanxi 716000, P.R. China, Department of Interventional Radiology, Affiliated Hospital of Yan'an University, Yan'an, Shanxi 716000, P.R. China, Department of Preventive Medicine, Medical College of Yan'an University, Yan'an, Shanxi 716000, P.R. China
  • Pages: 2913-2920
    |
    Published online on: February 5, 2019
       https://doi.org/10.3892/mmr.2019.9934
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Abstract

Previous studies have demonstrated that numerous tumor‑specific microRNAs (miRNAs) are upregulated or downregulated in hepatocellular carcinoma (HCC), and that their dysregulation is implicated in HCC occurrence and development. Therefore, investigation of crucial miRNAs involved in HCC oncogenesis and progression may provide novel insights into the therapy of patients with this malignant tumor. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assays were performed to detect tissue and cellular expression levels of miRNA‑663b (miR‑663b) in HCC. The effects of miR‑663b overexpression on the proliferation and invasion of HCC cells were examined using Cell Counting Kit‑8 and Transwell invasion assays, respectively. The direct target of miR‑663b in HCC cells was determined by bioinformatics analysis, luciferase reporter assay, RT‑qPCR and western blot analysis. It was observed that miR‑663b was expressed at low levels in HCC tissues and cell lines. miR‑663b upregulation suppressed the proliferative and invasive abilities of HCC cells. Additionally, Grb2‑associated binding 2 (GAB2) was regarded as a direct target gene of miR‑663b in HCC cells. Furthermore, GAB2 was overexpressed in HCC tissues, and overexpression of GAB2 was inversely correlated with levels of miR‑663b. GAB2 overexpression was able to rescue the suppressive effects of miR‑663b on HCC cells. These results demonstrated that this newly‑identified miR‑663b/GAB2 axis may be implicated in HCC occurrence and development.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Global Burden of Disease Cancer Collaboration, ; Fitzmaurice C, Dicker D, Pain A, Hamavid H, Moradi-Lakeh M, MacIntyre MF, Allen C, Hansen G, Woodbrook R, et al: The global burden of cancer 2013. JAMA Oncol. 1:505–527. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Cabrera R and Nelson DR: Review article: The management of hepatocellular carcinoma. Aliment Pharmacol Ther. 31:461–476. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Marrero JA and Welling T: Modern diagnosis and management of hepatocellular carcinoma. Clin Liver Dis. 13:233–247. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Forner A, Hessheimer AJ, Isabel Real M and Bruix J: Treatment of hepatocellular carcinoma. Crit Rev Oncol Hematol. 60:89–98. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Ishizawa T, Hasegawa K, Aoki T, Takahashi M, Inoue Y, Sano K, Imamura H, Sugawara Y, Kokudo N and Makuuchi M: Neither multiple tumors nor portal hypertension are surgical contraindications for hepatocellular carcinoma. Gastroenterology. 134:1908–1916. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Forner A, Llovet JM and Bruix J: Hepatocellular carcinoma. Lancet. 379:1245–1255. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Yu MC and Yuan JM: Environmental factors and risk for hepatocellular carcinoma. Gastroenterology 127 (5 Suppl 1). S72–S78. 2004.

9 

Borchert GM, Lanier W and Davidson BL: RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol. 13:1097–1101. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Klingenberg M, Matsuda A, Diederichs S and Patel T: Non-coding RNA in hepatocellular carcinoma: Mechanisms, biomarkers and therapeutic targets. J Hepatol. 67:603–618. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Weidle UH, Birzele F, Kollmorgen G and Nopora A: Potential microRNA-related targets for therapeutic intervention with ovarian cancer metastasis. Cancer Genomics Proteomics. 15:1–15. 2018.PubMed/NCBI

13 

Celano M, Rosignolo F, Maggisano V, Pecce V, Iannone M, Russo D and Bulotta S: MicroRNAs as Biomarkers in Thyroid Carcinoma. Int J Genomics. 2017:64965702017. View Article : Google Scholar : PubMed/NCBI

14 

Irmak-Yazicioglu MB: Mechanisms of microRNA deregulation and microRNA targets in gastric cancer. Oncol Res Treat. 39:136–139. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Zhang Y, Yang L, Wang S, Liu Z and Xiu M: MiR-29a suppresses cell proliferation by targeting SIRT1 in hepatocellular carcinoma. Cancer Biomark. 22:151–159. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Li H, Wang H and Ren Z: MicroRNA-214-5p inhibits the invasion and migration of hepatocellular carcinoma cells by targeting Wiskott-Aldrich syndrome like. Cell Physiol Biochem. 46:757–764. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Wu M, Huang C, Huang X, Liang R, Feng Y and Luo X: MicroRNA-144-3p suppresses tumor growth and angiogenesis by targeting SGK3 in hepatocellular carcinoma. Oncol Rep. 38:2173–2181. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Hayes CN and Chayama K: MicroRNAs as biomarkers for liver disease and hepatocellular carcinoma. Int J Mol Sci. 17:2802016. View Article : Google Scholar : PubMed/NCBI

19 

Anwar SL and Lehmann U: MicroRNAs: Emerging Novel Clinical Biomarkers for Hepatocellular Carcinomas. J Clin Med. 4:1631–1650. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Liu Z, Wang Y, Dou C, Sun L, Li Q, Wang L, Xu Q, Yang W, Liu Q and Tu K: MicroRNA-1468 promotes tumor progression by activating PPAR-gamma-mediated AKT signaling in human hepatocellular carcinoma. J Exp Clin Cancer Res. 37:492018. View Article : Google Scholar : PubMed/NCBI

21 

Liang S, Zhang N, Deng Y, Chen L, Zhang Y, Zheng Z, Luo W, Lv Z, Li S and Xu T: miR-663b promotes tumor cell proliferation, migration and invasion in nasopharyngeal carcinoma through targeting TUSC2. Exp Ther Med. 14:1095–1103. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Du M, Shi D, Yuan L, Li P, Chu H, Qin C, Yin C, Zhang Z and Wang M: Circulating miR-497 and miR-663b in plasma are potential novel biomarkers for bladder cancer. Sci Rep. 5:104372015. View Article : Google Scholar : PubMed/NCBI

23 

Shu Y, Ye W, Gu YL and Sun P: Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression. Bratisl Lek Listy. 119:41–46. 2018.PubMed/NCBI

24 

Wang YL, Shen Y, Xu JP, Han K, Zhou Y, Yang S, Yin JY, Min DL and Hu HY: Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b. Acta Pharmacol Sin. 38:1394–1400. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Faria SC, Szklaruk J, Kaseb AO, Hassabo HM and Elsayes KM: TNM/Okuda/Barcelona/UNOS/clip international multidisciplinary classification of hepatocellular carcinoma: Concepts, perspectives, and radiologic implications. Abdom Imaging. 39:1070–1087. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Hu X, He B, Zhou L, Xie H and Zheng S: Expression pattern and clinical significance of Gab2 protein in hepatocellular carcinoma. Clin Lab. 62:1087–1092. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Chen Y, Liu Q, Wu M, Li M, Ding H, Shan X, Liu J, Tao T, Ni R and Chen X: GAB2 promotes cell proliferation by activating the ERK signaling pathway in hepatocellular carcinoma. Tumour Biol. 37:11763–11773. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Cheng J, Zhong Y, Chen S, Sun Y, Huang L, Kang Y, Chen B, Chen G, Wang F, Tian Y, et al: Gab2 mediates hepatocellular carcinogenesis by integrating multiple signaling pathways. FASEB J. 31:5530–5542. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Zhang T, Liu W, Meng W, Zhao H, Yang Q, Gu SJ, Xiao CC, Jia CC and Fu BS: Downregulation of miR-542-3p promotes cancer metastasis through activating TGF-beta/Smad signaling in hepatocellular carcinoma. Onco Targets Ther. 11:1929–1939. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Cui H, Song R, Wu J, Wang W, Chen X and Yin J: MicroRNA-337 regulates the PI3K/AKT and Wnt/β-catenin signaling pathways to inhibit hepatocellular carcinoma progression by targeting high-mobility group AT-hook 2. Am J Cancer Res. 8:405–421. 2018.PubMed/NCBI

32 

Morishita A and Tsutomu M: MicroRNAs as possible biomarkers for hepatocellular carcinoma. Hepatol Res. 48:499–501. 2018. View Article : Google Scholar : PubMed/NCBI

33 

Cai H, An Y, Chen X, Sun D, Chen T, Peng Y, Zhu F, Jiang Y and He X: Epigenetic inhibition of miR-663b by long non-coding RNA HOTAIR promotes pancreatic cancer cell proliferation via up-regulation of insulin-like growth factor 2. Oncotarget. 7:86857–86870. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo L, Li B, Miao M, Yang J and Ji J: MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma. Mol Med Rep 19: 2913-2920, 2019.
APA
Guo, L., Li, B., Miao, M., Yang, J., & Ji, J. (2019). MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma. Molecular Medicine Reports, 19, 2913-2920. https://doi.org/10.3892/mmr.2019.9934
MLA
Guo, L., Li, B., Miao, M., Yang, J., Ji, J."MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma". Molecular Medicine Reports 19.4 (2019): 2913-2920.
Chicago
Guo, L., Li, B., Miao, M., Yang, J., Ji, J."MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma". Molecular Medicine Reports 19, no. 4 (2019): 2913-2920. https://doi.org/10.3892/mmr.2019.9934
Copy and paste a formatted citation
x
Spandidos Publications style
Guo L, Li B, Miao M, Yang J and Ji J: MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma. Mol Med Rep 19: 2913-2920, 2019.
APA
Guo, L., Li, B., Miao, M., Yang, J., & Ji, J. (2019). MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma. Molecular Medicine Reports, 19, 2913-2920. https://doi.org/10.3892/mmr.2019.9934
MLA
Guo, L., Li, B., Miao, M., Yang, J., Ji, J."MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma". Molecular Medicine Reports 19.4 (2019): 2913-2920.
Chicago
Guo, L., Li, B., Miao, M., Yang, J., Ji, J."MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma". Molecular Medicine Reports 19, no. 4 (2019): 2913-2920. https://doi.org/10.3892/mmr.2019.9934
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