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Article

Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice

  • Authors:
    • Libo Peng
    • Zikang Xie
    • Jie Pei
    • Bing Wang
    • Yi Gao
    • Yuxing Qu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Changzhou Traditional Chinese Medicine Hospital, Changzhou, Jiangsu 213003, P.R. China
  • Pages: 2876-2882
    |
    Published online on: February 5, 2019
       https://doi.org/10.3892/mmr.2019.9936
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Abstract

Recent studies have suggested that puerarin may impede osteoclastogenesis and facilitate bone regeneration, in addition to attenuating tissue inflammation. The present study investigated the therapeutic effects of puerarin on inflammatory responses and monocyte recruitment in in vitro and in vivo osteoarthritis (OA) models. Puerarin treatment increased the proliferation of OA chondrocytes, as determined by Cell Counting Kit‑8 assay. In addition, the present results suggested that puerarin suppressed the interleukin‑1β‑induced production of inflammatory cytokines in OA chondrocytes and monocytes/macrophages, as assessed by ELISA. In a mouse model of mono‑iodoacetate‑induced OA, the present histological analyses suggested that administration with puerarin attenuated the inflammatory profile of OA joints and reduced cartilage destruction. Using flow cytometry, a decreased number of myeloid‑derived C‑C chemokine receptor 2+/lymphocyte Ag 6C+ monocytes was identified in the blood of OA mice treated with puerarin compared with control OA mice. Furthermore, quantitative real‑time polymerase chain reaction analysis suggested that puerarin treatment decreased C‑C chemokine ligand 2 expression in arthritic tissues. Collectively, the results suggested that puerarin treatment limited the recruitment of inflammatory monocytes. In summary, the present study provided pre‑clinical evidence that puerarin may serve as a potential target in the treatment of OA.
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Copy and paste a formatted citation
Spandidos Publications style
Peng L, Xie Z, Pei J, Wang B, Gao Y and Qu Y: Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice. Mol Med Rep 19: 2876-2882, 2019.
APA
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., & Qu, Y. (2019). Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice. Molecular Medicine Reports, 19, 2876-2882. https://doi.org/10.3892/mmr.2019.9936
MLA
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., Qu, Y."Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice". Molecular Medicine Reports 19.4 (2019): 2876-2882.
Chicago
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., Qu, Y."Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice". Molecular Medicine Reports 19, no. 4 (2019): 2876-2882. https://doi.org/10.3892/mmr.2019.9936
Copy and paste a formatted citation
x
Spandidos Publications style
Peng L, Xie Z, Pei J, Wang B, Gao Y and Qu Y: Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice. Mol Med Rep 19: 2876-2882, 2019.
APA
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., & Qu, Y. (2019). Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice. Molecular Medicine Reports, 19, 2876-2882. https://doi.org/10.3892/mmr.2019.9936
MLA
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., Qu, Y."Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice". Molecular Medicine Reports 19.4 (2019): 2876-2882.
Chicago
Peng, L., Xie, Z., Pei, J., Wang, B., Gao, Y., Qu, Y."Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice". Molecular Medicine Reports 19, no. 4 (2019): 2876-2882. https://doi.org/10.3892/mmr.2019.9936
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