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Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer

  • Authors:
    • Vahinipriya Manoharan
    • Eric Hamilton Karunanayake
    • Kamani Hemamala Tennekoon
    • Sumadee De Silva
    • Kanishka De Silva
    • Preethika Angunawela
    • John Lunec
  • View Affiliations / Copyright

    Affiliations: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 00300, Sri Lanka, Department of Oncosurgery, National Cancer Institute, Maharagama 10280, Sri Lanka, Department of Pathology, Faculty of Medicine, University of Colombo, Colombo 00800, Sri Lanka, Northern Institute for Cancer Research, Newcastle University, Newcastle NE2 4AD, UK
    Copyright: © Manoharan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 2781-2791
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    Published online on: February 11, 2019
       https://doi.org/10.3892/mmr.2019.9948
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Abstract

Head and neck cancer (HNC) is the leading cancer in Sri Lankan males and second most common cancer among Sri Lankan females. This is the first study, to the best of our knowledge, that has focused on investigating the association between TP53 somatic DNA variants, with p53 protein expression and risk factors in a cohort of Sri Lankan patients with HNC. A total of 44 patients with cancer and 20 healthy controls were studied. In total, 36 genomic DNA sequence variants were found, including several novel variants (two deletions in exons 4 and 6, two in the 3' untranslated region and several intronic variants). A total of 14 tumour samples carried pathogenic TP53 mutations. A random selection of 24 samples was analysed immunohistochemically for p53 protein expression. All the samples with point missense variants were strongly immuno‑positive, whereas, samples with nonsense and frameshift TP53 variants were immuno‑negative for p53 immunohistochemical staining. Although, the human papilloma virus is a known risk factor for HNC, results from the present study identified an absence or lower level of infection in the Sri Lankan cohort.
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Copy and paste a formatted citation
Spandidos Publications style
Manoharan V, Karunanayake EH, Tennekoon KH, De Silva S, De Silva K, Angunawela P and Lunec J: Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer. Mol Med Rep 19: 2781-2791, 2019.
APA
Manoharan, V., Karunanayake, E.H., Tennekoon, K.H., De Silva, S., De Silva, K., Angunawela, P., & Lunec, J. (2019). Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer. Molecular Medicine Reports, 19, 2781-2791. https://doi.org/10.3892/mmr.2019.9948
MLA
Manoharan, V., Karunanayake, E. H., Tennekoon, K. H., De Silva, S., De Silva, K., Angunawela, P., Lunec, J."Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer". Molecular Medicine Reports 19.4 (2019): 2781-2791.
Chicago
Manoharan, V., Karunanayake, E. H., Tennekoon, K. H., De Silva, S., De Silva, K., Angunawela, P., Lunec, J."Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer". Molecular Medicine Reports 19, no. 4 (2019): 2781-2791. https://doi.org/10.3892/mmr.2019.9948
Copy and paste a formatted citation
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Spandidos Publications style
Manoharan V, Karunanayake EH, Tennekoon KH, De Silva S, De Silva K, Angunawela P and Lunec J: Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer. Mol Med Rep 19: 2781-2791, 2019.
APA
Manoharan, V., Karunanayake, E.H., Tennekoon, K.H., De Silva, S., De Silva, K., Angunawela, P., & Lunec, J. (2019). Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer. Molecular Medicine Reports, 19, 2781-2791. https://doi.org/10.3892/mmr.2019.9948
MLA
Manoharan, V., Karunanayake, E. H., Tennekoon, K. H., De Silva, S., De Silva, K., Angunawela, P., Lunec, J."Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer". Molecular Medicine Reports 19.4 (2019): 2781-2791.
Chicago
Manoharan, V., Karunanayake, E. H., Tennekoon, K. H., De Silva, S., De Silva, K., Angunawela, P., Lunec, J."Nucleotide variants and protein expression of TP53 in a Sri Lankan cohort of patients with head and neck cancer". Molecular Medicine Reports 19, no. 4 (2019): 2781-2791. https://doi.org/10.3892/mmr.2019.9948
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