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Article

circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis

  • Authors:
    • Yikun Qu
    • Penghui Dou
    • Ming Hu
    • Jian Xu
    • Weibin Xia
    • Hongmei Sun
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China, Department of Radiation and Chemotherapy, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China, Department of Oncology, Oncology Hospital of Jiamusi City, Jiamusi, Heilongjiang 154002, P.R. China
  • Pages: 3314-3320
    |
    Published online on: February 18, 2019
       https://doi.org/10.3892/mmr.2019.9965
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Abstract

Chondrocyte extracellular matrix‑related circular RNAs (circRNA‑CER) have been demonstrated to be involved in various diseases. However, its role in the development of human breast cancer is not clearly understood. The aims of the present study were to assess circRNA‑CER expression in paired cancer tissue and adjacent non‑tumor tissue from 24 patients with breast cancer, and to explore the roles and mechanisms by which circRNA‑CER mediates the malignant progression of breast cancer cells. The results revealed that circRNA‑CER and matrix metalloproteinase 13 (MMP13) were upregulated, whereas miR‑136 was downregulated in breast cancer tissues compared with adjacent non‑tumor tissues. In vitro silencing of circRNA‑CER using small interfering RNA (siRNA) had inhibitory effects on MCF‑7 breast cancer cell proliferation and migration, and similar results were obtained following overexpression of microRNA (miR)‑136 in MCF‑7 cells by transfection with miR‑136 mimics. The subsequent mechanistic study revealed that the expression levels of MMP13 were significantly lower in MCF‑7 cells following transfection with miR‑136 mimics, and silencing of circRNA‑CER enhanced miR‑136 and decreased MMP13 expression levels. Furthermore, silencing of miR‑136 by transfection with miR‑136 inhibitors resulted in an increase in MCF‑7 cell proliferation and migration. miR‑136 inhibitor‑derived biological effects were reversed by co‑transfection of cells with miR‑136 inhibitors and circRNA‑CER siRNA. Taken together, the present results suggested that circRNA‑CER may serve an important role in the progression of breast cancer by regulating the activity of the miR‑136/MMP13 axis, and may be a potential biomarker for the prediction and treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Qu Y, Dou P, Hu M, Xu J, Xia W and Sun H: circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis. Mol Med Rep 19: 3314-3320, 2019.
APA
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., & Sun, H. (2019). circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis. Molecular Medicine Reports, 19, 3314-3320. https://doi.org/10.3892/mmr.2019.9965
MLA
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., Sun, H."circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis". Molecular Medicine Reports 19.4 (2019): 3314-3320.
Chicago
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., Sun, H."circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis". Molecular Medicine Reports 19, no. 4 (2019): 3314-3320. https://doi.org/10.3892/mmr.2019.9965
Copy and paste a formatted citation
x
Spandidos Publications style
Qu Y, Dou P, Hu M, Xu J, Xia W and Sun H: circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis. Mol Med Rep 19: 3314-3320, 2019.
APA
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., & Sun, H. (2019). circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis. Molecular Medicine Reports, 19, 3314-3320. https://doi.org/10.3892/mmr.2019.9965
MLA
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., Sun, H."circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis". Molecular Medicine Reports 19.4 (2019): 3314-3320.
Chicago
Qu, Y., Dou, P., Hu, M., Xu, J., Xia, W., Sun, H."circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis". Molecular Medicine Reports 19, no. 4 (2019): 3314-3320. https://doi.org/10.3892/mmr.2019.9965
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