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miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog

  • Authors:
    • Xiaoyuan Wang
    • Zhijian Li
    • Jie Bai
    • Wuqi Song
    • Fengmin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Microbiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogenic Biology, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3132-3138
    |
    Published online on: February 20, 2019
       https://doi.org/10.3892/mmr.2019.9973
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Abstract

Glaucoma is one of the leading causes of blindness. Previous studies have indicated that the oxidative stress‑induced apoptosis of trabecular meshwork cells (TMCs) may serve a key role in the pathogenesis of glaucoma, and that micro RNA(miR)‑17‑5p may be involved in this process. However, the specific mechanisms require further investigation. The aim of the present study was to investigate the effects of miR‑17‑5p on the proliferation and apoptosis of human TMCs (HTMCs) in response to oxidative stress. It was observed that exposure to H2O2 induced a significant decrease in the proliferation and a marked increase in the apoptosis of HTMCs. H2O2 exposure also suppressed the expression of miR‑17‑5p and promoted the expression of phosphatase and tensin homolog (PTEN). Furthermore, transient overexpression of miR‑17‑5p induced a significant increase in the proliferation and a significant decrease in the apoptosis of HTMCs by affecting the expression of PTEN, and the apoptosis‑related proteins B‑cell lymphoma‑associated X protein (Bax), B‑cell lymphoma‑extra large (Bcl‑xL) and B‑cell lymphoma‑2 (Bcl‑2). However, knockdown of miR‑17‑5p demonstrated the opposite results. The results of a dual luciferase reporter assay demonstrated that PTEN may be a direct target of miR‑17‑5p. In conclusion, miR‑17‑5p was downregulated in HTMCs under oxidative conditions, and miR‑17‑5p may regulate the apoptosis of HTMCs by targeting PTEN. These results provide a novel theoretical basis and potential therapeutic target for the treatment of glaucoma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Li Z, Bai J, Song W and Zhang F: miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog. Mol Med Rep 19: 3132-3138, 2019.
APA
Wang, X., Li, Z., Bai, J., Song, W., & Zhang, F. (2019). miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog. Molecular Medicine Reports, 19, 3132-3138. https://doi.org/10.3892/mmr.2019.9973
MLA
Wang, X., Li, Z., Bai, J., Song, W., Zhang, F."miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog". Molecular Medicine Reports 19.4 (2019): 3132-3138.
Chicago
Wang, X., Li, Z., Bai, J., Song, W., Zhang, F."miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog". Molecular Medicine Reports 19, no. 4 (2019): 3132-3138. https://doi.org/10.3892/mmr.2019.9973
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Li Z, Bai J, Song W and Zhang F: miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog. Mol Med Rep 19: 3132-3138, 2019.
APA
Wang, X., Li, Z., Bai, J., Song, W., & Zhang, F. (2019). miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog. Molecular Medicine Reports, 19, 3132-3138. https://doi.org/10.3892/mmr.2019.9973
MLA
Wang, X., Li, Z., Bai, J., Song, W., Zhang, F."miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog". Molecular Medicine Reports 19.4 (2019): 3132-3138.
Chicago
Wang, X., Li, Z., Bai, J., Song, W., Zhang, F."miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog". Molecular Medicine Reports 19, no. 4 (2019): 3132-3138. https://doi.org/10.3892/mmr.2019.9973
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