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Article

NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model

  • Authors:
    • Yanhua Lan
    • Huizhi Xie
    • Yang Shi
    • Qianrui Jin
    • Xiaolei Zhang
    • Yu Wang
    • Zhijian Xie
  • View Affiliations / Copyright

    Affiliations: Department of Oral Medicine, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China, Department of General Dentistry, Hangzhou Dental Hospital, Hangzhou, Zhejiang 310006, P.R. China, Department of Implantology, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 3291-3297
    |
    Published online on: February 20, 2019
       https://doi.org/10.3892/mmr.2019.9975
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Abstract

Osteomyelitis, which is characterized by progressive inflammatory bone destruction and resorption, is a difficult‑to‑treat infection. Staphylococcus aureus (S. aureus) is one of the major causes of this disease. This pathogenic microorganism possesses several characteristics, which facilitate its involvement in the occurrence and progression of osteomyelitis. A cell‑permeable peptide inhibitor of the IκB kinase complex, the nuclear factor (NF)‑κB essential modulator‑binding domain (NBD) peptide, has been reported to block osteoclastogenesis and may be considered a potential strategy for preventing inflammatory bone resorption. However, it remains to be determined as to whether the NBD peptide can regulate inflammation and bone resorption in S. aureus‑induced osteomyelitis. In order to investigate the role of NBD in S. aureus‑induced osteomyelitis, the present study obtained the NBD peptide, and confirmed that it inhibited receptor activator of NF‑κB ligand‑induced osteoclastogenesis in vitro. Subsequently, a bone defect was generated and S. aureus was injected into the mandible of experimental animals, in order to establish an in vivo osteomyelitis model. The present study analyzed the following three experimental groups: Untreated, treated with debridement, and treated with debridement plus NBD peptide administration. The results revealed that treatment with the NBD peptide reduced the bone defect in a 3‑dimensional manner, and reduced bone resorption. To the best of our knowledge, the present study is the first to demonstrate that, in a model of osteomyelitis caused by S. aureus, the NBD peptide serves a role in inhibiting osteolysis and promoting bone remodeling in the direction of osteogenesis. The effects were better than those produced by debridement alone, thus suggesting that it may have promising therapeutic potential in osteomyelitis.
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1 

Hajishengallis G, Moutsopoulos NM, Hajishengallis E and Chavakis T: Immune and regulatory functions of neutrophils in inflammatory bone loss. Semin Immunol. 28:146–158. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Inzana JA, Schwarz EM, Kates SL and Awad HA: Biomaterials approaches to treating implant-associated osteomyelitis. Biomaterials. 81:58–71. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Wang R, Braughton KR, Kretschmer D, Bach TH, Queck SY, Li M, Kennedy AD, Dorward DW, Klebanoff SJ, Peschel A, et al: Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat Med. 13:1510–1514. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Park OJ, Kim J, Yang J, Yun CH and Han SH: Muramyl dipeptide, a shared structural motif of peptidoglycans, is a novel inducer of bone formation through induction of Runx2. J Bone Miner Res. 32:1455–1468. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Verdrengh M, Bokarewa M, Ohlsson C, Stolina M and Tarkowski A: RANKL-targeted therapy inhibits bone resorption in experimental Staphylococcus aureus-induced arthritis. Bone. 46:752–758. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Xing L, Xiu Y and Boyce BF: Osteoclast fusion and regulation by RANKL-dependent and independent factors. World J Orthop. 3:212–222. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Zuo C, Huang Y, Bajis R, Sahih M, Li YP, Dai K and Zhang X: Osteoblastogenesis regulation signals in bone remodeling. Osteoporos Int. 23:1653–1663. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Gross C, Weber M, Creutzburg K, Möbius P, Preidl R, Amann K and Wehrhan F: Osteoclast profile of medication-related osteonecrosis of the jaw secondary to bisphosphonate therapy: A comparison with osteoradionecrosis and osteomyelitis. J Transl Med. 15:1282017. View Article : Google Scholar : PubMed/NCBI

9 

Aurore V, Caldana F, Blanchard M, Kharoubi Hess S, Lannes N, Mantel PY, Filgueira L and Walch M: Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts. Nanomedicine. 14:601–607. 2018. View Article : Google Scholar : PubMed/NCBI

10 

May MJ, D'Acquisto F, Madge LA, Glöckner J, Pober JS and Ghosh S: Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex. Science. 289:1550–1554. 2000. View Article : Google Scholar : PubMed/NCBI

11 

May MJ, Marienfeld RB and Ghosh S: Characterization of the Ikappa B-kinase NEMO binding domain. J Biol Chem. 277:45992–46000. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Kleppe M, Koche R, Zou L, van Galen P, Hill CE, Dong L, De Groote S, Papalexi E, Hanasoge Somasundara AV, Cordner K, et al: Dual targeting of oncogenic activation and inflammatory signaling increases therapeutic efficacy in myeloproliferative neoplasms. Cancer Cell. 33:785–787. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Luo G, Li F, Li X, Wang ZG and Zhang B: TNF-α and RANKL promote osteoclastogenesis by upregulating RANK via the NF-κB pathway. Mol Med Rep. 17:6605–6611. 2018.PubMed/NCBI

14 

Oeckinghaus A and Ghosh S: The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol. 1:a0000342009. View Article : Google Scholar : PubMed/NCBI

15 

Vancurova I and Vancura A: Regulation and function of nuclear IκBα in inflammation and cancer. Am J Clin Exp Immunol. 1:56–66. 2012.PubMed/NCBI

16 

Dai S, Hirayama T, Abbas S and Abu-Amer Y: The IkappaB kinase (IKK) inhibitor, NEMO-binding domain peptide, blocks osteoclastogenesis and bone erosion in inflammatory arthritis. J Biol Chem. 279:37219–37222. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Strickland I and Ghosh S: Use of cell permeable NBD peptides for suppression of inflammation. Ann Rheum Dis. 65 (Suppl 3):iii75–iii82. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Jimi E, Aoki K, Saito H, D'Acquisto F, May MJ, Nakamura I, Sudo T, Kojima T, Okamoto F, Fukushima H, et al: Selective inhibition of NF-kappa B blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo. Nat Med. 10:617–624. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Wang Y, Dong G, Jeon HH, Elazizi M, La LB, Hameedaldeen A, Xiao E, Tian C, Alsadun S, Choi Y and Graves DT: FOXO1 mediates RANKL-induced osteoclast formation and activity. J Immunol. 194:2878–2887. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Swarnkar G and Abu-Amer Y: Regulation of NF-κB signaling in osteoclasts and myeloid progenitors. Methods Mol Biol. 1280:527–542. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Xu J, Wu HF, Ang ES, Yip K, Woloszyn M, Zheng MH and Tan RX: NF-kappaB modulators in osteolytic bone diseases. Cytokine Growth Factor Rev. 20:7–17. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Chacon GE, Bower DL, Larsen PE, McGlumphy EA and Beck FM: Heat production by 3 implant drill systems after repeated drilling and sterilization. J Oral Maxillofac Surg. 64:265–269. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Moskowitz JS, Blaisse MR, Samuel RE, Hsu HP, Harris MB, Martin SD, Lee JC, Spector M and Hammond PT: The effectiveness of the controlled release of gentamicin from polyelectrolyte multilayers in the treatment of Staphylococcus aureus infection in a rabbit bone model. Biomaterials. 31:6019–6030. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Sánchez AR, Sheridan PJ, Lohse C and Weaver A: Assessment of peripheral dual-energy X-ray absorptiometry measurements in peri-implant bone defects in dogs. J Periodontol. 75:658–662. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Kröpil P, Hakimi AR, Jungbluth P, Riegger C, Rubbert C, Miese F, Lanzman RS, Wild M, Schek A, Scherer A, et al: Cone beam CT in assessment of tibial bone defect healing: An animal study. Acad Radiol. 19:320–325. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Hotokezaka H, Sakai E, Kanaoka K, Saito K, Matsuo K, Kitaura H, Yoshida N and Nakayama K: U0126 and PD98059, specific inhibitors of MEK, accelerate differentiation of RAW264.7 cells into osteoclast-like cells. J Biol Chem. 277:47366–47372. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Wang Y, Liu X, Dou C, Cao Z, Liu C, Dong S and Fei J: Staphylococcal protein A promotes osteoclastogenesis through MAPK signaling during bone infection. J Cell Physiol. 232:2396–2406. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lan Y, Xie H, Shi Y, Jin Q, Zhang X, Wang Y and Xie Z: NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model. Mol Med Rep 19: 3291-3297, 2019.
APA
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., & Xie, Z. (2019). NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model. Molecular Medicine Reports, 19, 3291-3297. https://doi.org/10.3892/mmr.2019.9975
MLA
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., Xie, Z."NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model". Molecular Medicine Reports 19.4 (2019): 3291-3297.
Chicago
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., Xie, Z."NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model". Molecular Medicine Reports 19, no. 4 (2019): 3291-3297. https://doi.org/10.3892/mmr.2019.9975
Copy and paste a formatted citation
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Spandidos Publications style
Lan Y, Xie H, Shi Y, Jin Q, Zhang X, Wang Y and Xie Z: NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model. Mol Med Rep 19: 3291-3297, 2019.
APA
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., & Xie, Z. (2019). NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model. Molecular Medicine Reports, 19, 3291-3297. https://doi.org/10.3892/mmr.2019.9975
MLA
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., Xie, Z."NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model". Molecular Medicine Reports 19.4 (2019): 3291-3297.
Chicago
Lan, Y., Xie, H., Shi, Y., Jin, Q., Zhang, X., Wang, Y., Xie, Z."NEMO‑binding domain peptide ameliorates inflammatory bone destruction in a Staphylococcus aureus‑induced chronic osteomyelitis model". Molecular Medicine Reports 19, no. 4 (2019): 3291-3297. https://doi.org/10.3892/mmr.2019.9975
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