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Article

Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice

  • Authors:
    • Hye Jin Kim
    • Hyun Yang
    • Dong Ho Jung
    • Joo Tae Hwang
    • Byoung‑Seob Ko
  • View Affiliations / Copyright

    Affiliations: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea
  • Pages: 3321-3329
    |
    Published online on: February 22, 2019
       https://doi.org/10.3892/mmr.2019.9977
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Abstract

Cuscuta chinensis Lamak (CCL) has traditionally been used in Korea to treat sexual disorders and skin problems. The aim of the present study was to investigate the effects of CCL extract on surgical injury‑induced ischemia in the hind limbs of mice. Specifically, female C57BL/6 mice were ovariectomized, and their hind‑limb vessels were ligated with surgical silk (6‑0) and excised. CCL (150 or 450 mg/kg/BW) was then administered to the mice for 3 weeks, and the blood flow rate was evaluated using a laser Doppler system at ‑7, 0, 7, 14 and 21 days following hind‑limb ischemia. The serum expression profiles of angiogenic and inflammatory mediators were measured using an antibody array, and the transcript levels were reverse transcription‑quantitative polymerase chain reaction. The rate of hind limb blood flow was normalized to non‑ischemic lesions and revealed to be markedly elevated at 14 and 21 days following ischemia when compared with the vehicle group. The density of capillaries in the hind limbs was also significantly increased following treatment with CCL in a dose‑dependent manner. In addition, the transcriptional expression of angiogenetic factors were upregulated, whereas that of inflammatory cytokines were downregulated. Finally, vascular endothelial cell migration and tube formation were evaluated in vitro using human umbilical vein endothelial cells (HUVECs) and identified to be significantly increased following treatment with CCL. Overall, the results of the present study indicate that CCL extract exhibits therapeutic potential for the treatment of hind‑limb ischemia as it promotes peripheral angiogenic and anti‑inflammatory effects in mice.
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Copy and paste a formatted citation
Spandidos Publications style
Kim HJ, Yang H, Jung DH, Hwang JT and Ko BS: Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice. Mol Med Rep 19: 3321-3329, 2019.
APA
Kim, H.J., Yang, H., Jung, D.H., Hwang, J.T., & Ko, B. (2019). Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice. Molecular Medicine Reports, 19, 3321-3329. https://doi.org/10.3892/mmr.2019.9977
MLA
Kim, H. J., Yang, H., Jung, D. H., Hwang, J. T., Ko, B."Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice". Molecular Medicine Reports 19.4 (2019): 3321-3329.
Chicago
Kim, H. J., Yang, H., Jung, D. H., Hwang, J. T., Ko, B."Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice". Molecular Medicine Reports 19, no. 4 (2019): 3321-3329. https://doi.org/10.3892/mmr.2019.9977
Copy and paste a formatted citation
x
Spandidos Publications style
Kim HJ, Yang H, Jung DH, Hwang JT and Ko BS: Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice. Mol Med Rep 19: 3321-3329, 2019.
APA
Kim, H.J., Yang, H., Jung, D.H., Hwang, J.T., & Ko, B. (2019). Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice. Molecular Medicine Reports, 19, 3321-3329. https://doi.org/10.3892/mmr.2019.9977
MLA
Kim, H. J., Yang, H., Jung, D. H., Hwang, J. T., Ko, B."Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice". Molecular Medicine Reports 19.4 (2019): 3321-3329.
Chicago
Kim, H. J., Yang, H., Jung, D. H., Hwang, J. T., Ko, B."Ameliorating effects of Cuscuta chinensis Lamak extract on hind‑limb ischemia, and angiogenic‑ or inflammatory associated factors in ovariectomized mice". Molecular Medicine Reports 19, no. 4 (2019): 3321-3329. https://doi.org/10.3892/mmr.2019.9977
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