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Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway

  • Authors:
    • Yahui Hu
    • Xin Li
    • Guowei Huang
    • Jizuo Wang
    • Wei Lu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3095-3104
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    Published online on: February 22, 2019
       https://doi.org/10.3892/mmr.2019.9978
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Abstract

Bone mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self‑renewal and multidirectional differentiation. However, it is difficult to obtain high quality MSCs and to induce them to differentiate into neuron‑like cells. Fasudil, a Rho kinase inhibitor, exhibits therapeutic potential in spinal cord injuries and stroke. The present study investigated the effect of fasudil on the differentiation of MSCs into neuron‑like cells. MSCs were obtained from rat femur marrow, expanded in culture medium, and used at the third passage for subsequent experiments. MSCs were pre‑induced with 10 ng/ml basic fibroblast growth factor (bFGF) for 24 h, which was followed by induction with fasudil. A control untreated group and a group treated with fasudil + XAV939, a Wnt/β‑catenin pathway inhibitor, were also used in the present study. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blot analysis and immunofluorescence staining were performed in order to detect neuron‑specific markers, including neuron‑specific enolase (NSE), nestin and neurofilament‑M (NF‑M). Following induction with fasudil, neuron‑like cell morphology was observed. In the fasudil + XAV939 and control groups, no obvious changes in cell shape were observed. The results of RT‑qPCR, western blot analysis and immunofluorescence staining indicated that expression of the neuron‑specific markers NSE, nestin and NF‑M was detected in the fasudil group. The differentiation of MSCs into neuron‑like cells induced by fasudil was eliminated when the Wnt/β‑catenin pathway was inhibited. The present study demonstrated that fasudil may induce MSCs to differentiate into neuron‑like cells, however further studies are required to determine the specific mechanisms involved in the effect of fasudil on the Wnt/β‑catenin pathway. In addition, further research is required to examine the functional characteristics of the induced neuron‑like cells, in order to establish their suitability for clinical treatments in the future.
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1 

Alvarez-Dolado M, Pardal R, Garcia-Verdugo JM, Fike JR, Lee HO, Pfeffer K, Lois C, Morrison SJ and Alvarez-Buylla A: Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature. 425:968–973. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Bjornson CR, Rietze RL, Reynolds BA, Magli MC and Vescovi AL: Turning brain into blood: A hematopoietic fate adopted by adult neural stem cells in vivo. Science. 283:534–537. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Eglitis MA and Mezey E: Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice. Proc Natl Acad Sci USA. 94:4080–4085. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Petersen BE, Bowen WC, Patrene KD, Mars WM, Sullivan AK, Murase N, Boggs SS, Greenberger JS and Goff JP: Bone marrow as a potential source of hepatic oval cells. Science. 284:1168–1170. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Wu J, Sun Z, Sun HS, Wu J, Weisel RD, Keating A, Li ZH, Feng ZP and Li RK: Intravenously administered bone marrow cells migrate to damaged brain tissue and improve neural function in ischemic rats. Cell Transplant. 16:993–1005. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Yamazaki Y, Kanno H, Maeda K, Yoshida T, Kobayashi N, Kubo A, Yamaguchi Y and Saito T: Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats. Neuroreport. 21:287–292. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Liu W, Jiang X, Fu X, Cui S, Du M, Cai Y and Xu R: Bone marrow stromal cells can be delivered to the site of traumatic brain injury via intrathecal transplantation in rabbits. Neurosci Lett. 434:160–164. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Chartoff EH, Damez-Werno D, Sonntag KC, Hassinger L, Kaufmann DE, Peterson J, McPhie D, Cataldo AM and Cohen BM: Detection of intranasally delivered bone marrow-derived mesenchymal stromal cells in the lesioned mouse brain: A cautionary report. Stem Cells Int. 2011:5865862011. View Article : Google Scholar : PubMed/NCBI

9 

Wakao S, Hayashi T, Kitada M, Kohama M, Matsue D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, et al: Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration. Exp Neurol. 223:537–547. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Cuevas P, Carceller F, Dujovny M, Garcia-Gómez I, Cuevas B, González-Corrochano R, Diaz-González D and Reimers D: Peripheral nerve regeneration by bone marrow stromal cells. Neurol Res. 24:634–638. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Shimizu S, Kitada M, Ishikawa H, Itokazu Y, Wakao S and Dezawa M: Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property. Biochem Biophys Res Commun. 359:915–920. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Choong PF, Mok PL, Cheong SK, Leong CF and Then KY: Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro. Cytotherapy. 9:170–183. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Kondo T, Johnson SA, Yoder MC, Romand R and Hashino E: Sonic hedgehog and retinoic acid synergistically promote sensory fate specification from bone marrow-derived pluripotent stem cells. Proc Natl Acad Sci USA. 102:4789–4794. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Song S and Sanchez-Ramos J: Preparation of neural progenitors from bone marrow and umbilical cord blood. Methods Mol Biol. 438:123–134. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Chen Y, Teng FY and Tang BL: Coaxing bone marrow stromal mesenchymal stem cells towards neuronal differentiation: Progress and uncertainties. Cell Mol Life Sci. 63:1649–1657. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Qin T, Fang F, Song M, Li R, Ma Z and Ma S: Umbelliferone reverses depression-like behavior in chronic unpredictable mild stress-induced rats by attenuating neuronal apoptosis via regulating ROCK/Akt pathway. Behav Brain Res. 317:147–156. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Li Q, Huang XJ, He W, Ding J, Jia JT, Fu G, Wang HX and Guo LJ: Neuroprotective potential of fasudil mesylate in brain ischemia-reperfusion injury of rats. Cell Mol Neurobiol. 29:169–180. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Alokam R, Singhal S, Srivathsav GS, Garigipati S, Puppala S, Sriram D and Perumal Y: Design of dual inhibitors of ROCK-I and NOX2 as potential leads for the treatment of neuroinflammation associated with various neurological diseases including autism spectrum disorder. Mol Biosyst. 11:607–617. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Li L, Zhi D, Shen Y, Liu K, Li H and Chen J: Effects of CC-chemokine receptor 5 on ROCK2 and P-MLC2 expression after focal cerebral ischaemia-reperfusion injury in rats. Brain Inj. 30:468–473. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Koyanagi M, Takahashi J, Arakawa Y, Doi D, Fukuda H, Hayashi H, Narumiya S and Hashimoto N: Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res. 86:270–280. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Satoh S, Toshima Y, Hitomi A, Ikegaki I, Seto M and Asano T: Wide therapeutic time window for Rho-kinase inhibition therapy in ischemic brain damage in a rat cerebral thrombosis model. Brain Res. 1193:102–108. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Ding J, Li QY, Yu JZ, Wang X, Sun CH, Lu CZ and Xiao BG: Fasudil, a Rho kinase inhibitor, drives mobilization of adult neural stem cells after hypoxia/reoxygenation injury in mice. Mol Cell Neurosci. 43:201–208. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Lee HS, Kim KS, Lim HS, Choi M, Kim HK, Ahn HY, Shin JC and Joe YA: Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model. J Cell Biochem. 116:310–319. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Zhao EY, Wang LD, Wen QQ, Guan WJ, Jing LJ, Peng T, Wen GQ and Jia YJ: Effect of notch signaling on differentiation of rat marrow mesenchymal stem cells into neurons induced by fasudil hydrochloride. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 26:428–432. 2010.(In Chinese). PubMed/NCBI

25 

Zhao EY, Jia YJ, Wang DM, Wen GQ, Guan WJ, Jing LJ and Deng YD: Effect of p65 gene inhibited by siRNA on differention of rat marrow mesenchymal stem cells into neurons. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 31:254–258. 2015.(In Chinese). PubMed/NCBI

26 

Wu R, Tang Y, Zang W, Wang Y, Li M, Du Y, Zhao G and Xu Y: MicroRNA-128 regulates the differentiation of rat bone mesenchymal stem cells into neuron-like cells by Wnt signaling. Mol Cell Biochem. 387:151–158. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Yu Q, Liu L, Duan Y, Wang Y, Xuan X, Zhou L and Liu W: Wnt/β-catenin signaling regulates neuronal differentiation of mesenchymal stem cells. Biochem Biophys Res Commun. 439:297–302. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Li L, Tam L, Liu L, Jin T and Ng DS: Wnt-signaling mediates the anti-adipogenic action of lysophosphatidic acid through cross talking with the Rho/Rho associated kinase ROCK) pathway. Biochem Cell Biol. 89:515–521. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Zhao Y, Zhang Q, Xi J, Xiao B, Li Y and Ma C: Neuroprotective effect of fasudil on inflammation through PI3K/Akt and Wnt/β-catenin dependent pathways in a mice model of Parkinson's disease. Int J Clin Exp Pathol. 8:2354–2364. 2015.PubMed/NCBI

30 

Zhao YF, Zhang Q, Xi JY, Li YH, Ma CG and Xiao BG: Multitarget intervention of Fasudil in the neuroprotection of dopaminergic neurons in MPTP-mouse model of Parkinson's disease. J Neurol Sci. 353:28–37. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Long X, Olszewski M, Huang W and Kletzel M: Neural cell differentiation in vitro from adult human bone marrow mesenchymal stem cells. Stem Cells Dev. 14:65–69. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Ge L, Liu K, Liu Z and Lu M: Co-transplantation of autologous OM-MSCs and OM-OECs: A novel approach for spinal cord injury. Rev Neurosci. 27:259–270. 2016.PubMed/NCBI

34 

Kumagai G, Tsoulfas P, Toh S, McNiece I, Bramlett HM and Dietrich WD: Genetically modified mesenchymal stem cells (MSCs) promote axonal regeneration and prevent hypersensitivity after spinal cord injury. Exp Neurol. 248:369–380. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Kopen GC, Prockop DJ and Phinney DG: Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci USA. 96:10711–10716. 1999. View Article : Google Scholar : PubMed/NCBI

36 

Bianco P, Robey PG and Simmons PJ: Mesenchymal stem cells: Revisiting history, concepts, and assays. Cell Stem Cell. 2:313–319. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Deng W, Obrocka M, Fischer I and Prockop DJ: In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Biochem Biophys Res Commun. 282:148–152. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Bae KS, Park JB, Kim HS, Kim DS, Park DJ and Kang SJ: Neuron-like differentiation of bone marrow-derived mesenchymal stem cells. Yonsei Med J. 52:401–412. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Kim BJ, Seo JH, Bubien JK and Oh YS: Differentiation of adult bone marrow stem cells into neuroprogenitor cells in vitro. Neuroreport. 13:1185–1188. 2002. View Article : Google Scholar : PubMed/NCBI

40 

Sagara J and Makino N: Glutathione induces neuronal differentiation in rat bone marrow stromal cells. Neurochem Res. 33:16–21. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Wang N, Xie K, Huo S, Zhao J, Zhang S and Miao J: Suppressing phosphatidylcholine-specific phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells. J Cell Biochem. 100:1548–1557. 2007. View Article : Google Scholar : PubMed/NCBI

42 

Pacary E, Legros H, Valable S, Duchatelle P, Lecocq M, Petit E, Nicole O and Bernaudin M: Synergistic effects of CoCl(2) and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells. J Cell Sci. 119:2667–2678. 2006. View Article : Google Scholar : PubMed/NCBI

43 

Pacary E, Petit E and Bernaudin M: Concomitant inhibition of prolyl hydroxylases and ROCK initiates differentiation of mesenchymal stem cells and PC12 towards the neuronal lineage. Biochem Biophys Res Commun. 377:400–406. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Dickson BJ: Rho GTPases in growth cone guidance. Curr Opin Neurobiol. 11:103–110. 2001. View Article : Google Scholar : PubMed/NCBI

45 

Sit ST and Manser E: Rho GTPases and their role in organizing the actin cytoskeleton. J Cell Sci. 124:679–683. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Begum R, Nur-E-Kamal MS and Zaman MA: The role of Rho GTPases in the regulation of the rearrangement of actin cytoskeleton and cell movement. Exp Mol Med. 36:358–366. 2004. View Article : Google Scholar : PubMed/NCBI

47 

Luo L: Rho GTPases in neuronal morphogenesis. Nat Rev Neurosci. 1:173–180. 2000. View Article : Google Scholar : PubMed/NCBI

48 

Bito H, Furuyashiki T, Ishihara H, Shibasaki Y, Ohashi K, Mizuno K, Maekawa M, Ishizaki T and Narumiya S: A critical role for a Rho-associated kinase, p160ROCK, in determining axon outgrowth in mammalian CNS neurons. Neuron. 26:431–441. 2000. View Article : Google Scholar : PubMed/NCBI

49 

Hirose M, Ishizaki T, Watanabe N, Uehata M, Kranenburg O, Moolenaar WH, Matsumura F, Maekawa M, Bito H and Narumiya S: Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells. J Cell Biol. 141:1625–1636. 1998. View Article : Google Scholar : PubMed/NCBI

50 

Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH and Jung JH: Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg. 34:537–542. 2005. View Article : Google Scholar : PubMed/NCBI

51 

Shin HK, Salomone S, Potts EM, Lee SW, Millican E, Noma K, Huang PL, Boas DA, Liao JK, Moskowitz MA and Ayata C: Rho-kinase inhibition acutely augments blood flow in focal cerebral ischemia via endothelial mechanisms. J Cereb Blood Flow Metab. 27:998–1009. 2007. View Article : Google Scholar : PubMed/NCBI

52 

Impellizzeri D, Mazzon E, Paterniti I, Esposito E and Cuzzocrea S: Effect of fasudil, a selective inhibitor of Rho kinase activity, in the secondary injury associated with the experimental model of spinal cord trauma. J Pharmacol Exp Ther. 343:21–33. 2012. View Article : Google Scholar : PubMed/NCBI

53 

Fournier AE, Takizawa BT and Strittmatter SM: Rho kinase inhibition enhances axonal regeneration in the injured CNS. J Neurosci. 23:1416–1423. 2003. View Article : Google Scholar : PubMed/NCBI

54 

Wang S, Kan Q, Sun Y, Han R, Zhang G, Peng T and Jia Y: Caveolin-1 regulates neural differentiation of rat bone mesenchymal stem cells into neurons by modulating Notch signaling. Int J Dev Neurosci. 31:30–35. 2013. View Article : Google Scholar : PubMed/NCBI

55 

Zechner D, Fujita Y, Hülsken J, Müller T, Walther I, Taketo MM, Crenshaw EB III, Birchmeier W and Birchmeier C: beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system. Dev Biol. 258:406–418. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hu Y, Li X, Huang G, Wang J and Lu W: Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway. Mol Med Rep 19: 3095-3104, 2019.
APA
Hu, Y., Li, X., Huang, G., Wang, J., & Lu, W. (2019). Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway. Molecular Medicine Reports, 19, 3095-3104. https://doi.org/10.3892/mmr.2019.9978
MLA
Hu, Y., Li, X., Huang, G., Wang, J., Lu, W."Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway". Molecular Medicine Reports 19.4 (2019): 3095-3104.
Chicago
Hu, Y., Li, X., Huang, G., Wang, J., Lu, W."Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway". Molecular Medicine Reports 19, no. 4 (2019): 3095-3104. https://doi.org/10.3892/mmr.2019.9978
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Y, Li X, Huang G, Wang J and Lu W: Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway. Mol Med Rep 19: 3095-3104, 2019.
APA
Hu, Y., Li, X., Huang, G., Wang, J., & Lu, W. (2019). Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway. Molecular Medicine Reports, 19, 3095-3104. https://doi.org/10.3892/mmr.2019.9978
MLA
Hu, Y., Li, X., Huang, G., Wang, J., Lu, W."Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway". Molecular Medicine Reports 19.4 (2019): 3095-3104.
Chicago
Hu, Y., Li, X., Huang, G., Wang, J., Lu, W."Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway". Molecular Medicine Reports 19, no. 4 (2019): 3095-3104. https://doi.org/10.3892/mmr.2019.9978
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