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Article

Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus

  • Authors:
    • Fu Xiong
    • Xiaotian Dai
    • Yu‑Xue Li
    • Rui Wei
    • Li An
    • Yajuan Wang
    • Zhenhong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pulmonology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, P.R. China, Department of Respiratory Medicine, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China, Department of Respiratory Medicine, North China University of Science and Technology of Clinical Medicine, Tangshan, Hebei 063000, P.R. China, Department of Nanlou Pulmonology and National Clinical Research Center of Geriatrics Disease, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
  • Pages: 3337-3344
    |
    Published online on: February 26, 2019
       https://doi.org/10.3892/mmr.2019.9988
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Abstract

Methicillin‑resistant Staphylococcus aureus (S. aureus; MRSA) is one of the most common bacterial pathogens and MRSA infections are characterized by high mortality rates. Antimicrobial peptides are considered one of the most promising drugs for the treatment of resistant strains of S. aureus. The present study aimed to examine the antimicrobial activity of L12 against numerous bacterial species using the broth microdilution method. Furthermore, the synergistic effect of L12 combined with various antibacterial drugs was tested, and its antibacterial mechanism was investigated by a checkerboard assay. The alterations in bacterial morphology were detected by electron microscopy, and biofilm formation and removal were tested by crystal violet staining. The present results suggested that L12 affected the growth of gram‑positive strains, particularly S. aureus. Electron microscopy analysis suggested that L12 may target the cell membrane, and L12 increased the antibacterial activity of vancomycin and levofloxacin, exerting a synergistic effect. However, the minimal inhibitory concentrations (MICs) of L12 were not correlated with antibiotic resistance, the strains resistant to more antibiotics were not more resistant to L12. A sub‑MIC of L12 was able to inhibit biofilm formation in a dose‑dependent manner; however, concentrations of L12 ≤10 times the MIC were not sufficient to degrade previously formed biofilm. Collectively, the present study suggested that L12 may represent a novel potential therapeutic molecule for the treatment of S. aureus infections.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong F, Dai X, Li YX, Wei R, An L, Wang Y and Chen Z: Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus. Mol Med Rep 19: 3337-3344, 2019.
APA
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., & Chen, Z. (2019). Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus. Molecular Medicine Reports, 19, 3337-3344. https://doi.org/10.3892/mmr.2019.9988
MLA
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., Chen, Z."Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus". Molecular Medicine Reports 19.4 (2019): 3337-3344.
Chicago
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., Chen, Z."Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus". Molecular Medicine Reports 19, no. 4 (2019): 3337-3344. https://doi.org/10.3892/mmr.2019.9988
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong F, Dai X, Li YX, Wei R, An L, Wang Y and Chen Z: Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus. Mol Med Rep 19: 3337-3344, 2019.
APA
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., & Chen, Z. (2019). Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus. Molecular Medicine Reports, 19, 3337-3344. https://doi.org/10.3892/mmr.2019.9988
MLA
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., Chen, Z."Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus". Molecular Medicine Reports 19.4 (2019): 3337-3344.
Chicago
Xiong, F., Dai, X., Li, Y., Wei, R., An, L., Wang, Y., Chen, Z."Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus". Molecular Medicine Reports 19, no. 4 (2019): 3337-3344. https://doi.org/10.3892/mmr.2019.9988
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