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Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221

  • Authors:
    • Chen Zhao
    • Jin‑Yang Chen
    • Wen‑Ming Peng
    • Bo Yuan
    • Qing Bi
    • You‑Jia Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Research and Development Department, Zhejiang Healthfuture Institute for Cell‑Based Applied Technology, Hangzhou, Zhejiang 310052, P.R. China, Department of Orthopedics, Tonglu TCM Hospital, Hangzhou, Zhejiang 311500, P.R. China, Department of Orthopedics, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1881-1889
    |
    Published online on: February 7, 2020
       https://doi.org/10.3892/mmr.2020.10982
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Abstract

Osteoarthritis (OA) is one of the most prevalent joint disorders globally. Patients suffering from OA are often obese and adiposity is linked to chronic inflammation. In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. First, it was tested whether patient‑derived ADSCs could differentiate into chondrogenic and osteogenic lineages. The ADSCs were then used as a source of exosomes. It was found that exosomes isolated from ADSCs, when co‑cultured with activated synovial fibroblasts, downregulated the expression of pro‑inflammatory markers interleukin (IL)‑6, NF‑κB and tumor necrosis factor‑α, while they upregulated the expression of the anti‑inflammatory cytokine IL‑10; without exosomes, the opposite observations were made. In addition, inflammation‑inflicted oxidative stress was induced in vitro by stimulating chondrocytes with H2O2. Treatment with exosomes protected articular chondrocytes from H2O2‑induced apoptosis. Furthermore, exosome treatment promoted chondrogenesis in periosteal cells and increased chondrogenic markers, including Collagen type II and β‑catenin; inhibition of Wnt/β‑catenin, using the antagonist ICG‑001, prevented exosome‑induced chondrogenesis. Periosteal cells treated with exosomes exhibited higher levels of microRNA (miR)‑145 and miR‑221. The upregulation of miR‑145 and miR‑221 was associated with the enhanced proliferation of periosteal cells and chondrogenic potential, respectively. The present study provided evidence in support for the use of patient‑derived exosomes, produced from ADSCs, for potential chondrogenic regeneration and subsequent amelioration of osteoarthritis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao C, Chen JY, Peng WM, Yuan B, Bi Q and Xu YJ: Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221. Mol Med Rep 21: 1881-1889, 2020.
APA
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., & Xu, Y. (2020). Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221. Molecular Medicine Reports, 21, 1881-1889. https://doi.org/10.3892/mmr.2020.10982
MLA
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., Xu, Y."Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221". Molecular Medicine Reports 21.4 (2020): 1881-1889.
Chicago
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., Xu, Y."Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221". Molecular Medicine Reports 21, no. 4 (2020): 1881-1889. https://doi.org/10.3892/mmr.2020.10982
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao C, Chen JY, Peng WM, Yuan B, Bi Q and Xu YJ: Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221. Mol Med Rep 21: 1881-1889, 2020.
APA
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., & Xu, Y. (2020). Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221. Molecular Medicine Reports, 21, 1881-1889. https://doi.org/10.3892/mmr.2020.10982
MLA
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., Xu, Y."Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221". Molecular Medicine Reports 21.4 (2020): 1881-1889.
Chicago
Zhao, C., Chen, J., Peng, W., Yuan, B., Bi, Q., Xu, Y."Exosomes from adipose‑derived stem cells promote chondrogenesis and suppress inflammation by upregulating miR‑145 and miR‑221". Molecular Medicine Reports 21, no. 4 (2020): 1881-1889. https://doi.org/10.3892/mmr.2020.10982
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