A novel corona core‑shell nanoparticle for enhanced intracellular drug delivery

Jiang,Jianwei; Zhong,Xiaoming; Zhang,Hongyan; Wang,Chunlei

doi:10.3892/mmr.2020.10998

A novel corona core‑shell nanoparticle for enhanced intracellular drug delivery

Authors:
- Jianwei Jiang
- Xiaoming Zhong
- Hongyan Zhang
- Chunlei Wang
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Affiliations: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
Published online on: February 24, 2020 https://doi.org/10.3892/mmr.2020.10998
Pages: 1965-1972

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Abstract

Drugs, including small molecule anticancer drugs, have to be delivered and released into the cytoplasm or nucleus in order to elicit a therapeutic effect. In the present study, a novel corona core‑shell nanoparticle was proposed for enhanced cellular uptake and light‑responsive intracellular release. Light‑responsive core‑shell nanoparticles, i.e., gold nanoparticles‑coated liposomes (CS), were prepared and further modified with hyaluronic acid (HA) (CCS). HA modification enhances the endocytosis of the nanoparticles by HA receptor‑expressing cells, while the plasmonic properties of gold nanoparticles enable their light‑triggered drug release. The results demonstrated that the uptake of CCS in HA receptor‑expressing MDA‑MB‑231R cells was significantly enhanced compared with unmodified CS. Nanosecond pulsed laser irradiation (700 nm; 100 mJ/cm2; 5 pulses) rapidly triggered the intracellular release of a fluorescent dye, 6‑carboxyfluorescein (6‑CF), from CCS and CS trapped in endolysosomes. The released 6‑CF was evenly distributed throughout the entire cytosol and nucleus. Finally, the cytotoxicity of doxorubicin towards MDA‑MB‑231 cells was significantly increased by CCS delivery upon pulsed laser irradiation. In conclusion, with enhanced cellular uptake and light‑triggered intracellular release, CCS significantly enhanced the therapeutic effects of doxorubicin, and may serve as a promising avenue for intracellular drug delivery.

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1	Dreaden EC, Austin LA, Mackey MA and El-Sayed MA: Size matters: Gold nanoparticles in targeted cancer drug delivery. Ther Deliv. 3:457–478. 2012. View Article : Google Scholar : PubMed/NCBI
2	Lukianova-Hleb EY, Ren X, Sawant RR, Wu X, Torchilin VP and Lapotko DO: On-demand intracellular amplification of chemoradiation with cancer-specific plasmonic nanobubbles. Nat Med. 20:778–784. 2014. View Article : Google Scholar : PubMed/NCBI
3	Meng FH, Cheng R, Deng C and Zhong ZY: Intracellular drug release nanosystems. Mater Today. 15:436–442. 2012. View Article : Google Scholar
4	Li X, Kang P, Chen Z, Lal S, Zhang L, Gassensmith JJ and Qin Z: Rock the nucleus: Significantly enhanced nuclear membrane permeability and gene transfection by plasmonic nanobubble induced nanomechanical transduction. Chem Commun (Camb). 54:2479–2482. 2018. View Article : Google Scholar : PubMed/NCBI
5	Sun H, Guo B, Cheng R, Meng F, Liu H and Zhong Z: Biodegradable micelles with sheddable poly(ethylene glycol) shells for triggered intracellular release of doxorubicin. Biomaterials. 30:6358–6366. 2009. View Article : Google Scholar : PubMed/NCBI
6	Martin AL, Bernas LM, Rutt BK, Foster PJ and Gillies ER: Enhanced cell uptake of superparamagnetic iron oxide nanoparticles functionalized with dendritic guanidines. Bioconjug Chem. 19:2375–2384. 2008. View Article : Google Scholar : PubMed/NCBI
7	Komin A, Russell LM, Hristova KA and Searson PC: Peptide-based strategies for enhanced cell uptake, transcellular transport, and circulation: Mechanisms and challenges. Adv Drug Deliv Rev. 110-111:52–64. 2017. View Article : Google Scholar : PubMed/NCBI
8	Li XY, Guo SY, Zhu CL, Zhu QL, Gan Y, Rantanen J, Rahbek UL, Hovgaard L and Yang MS: Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles. Biomaterials. 34:9678–9687. 2013. View Article : Google Scholar : PubMed/NCBI
9	Mohanraj VJ, Barnes TJ and Prestidge CA: Silica nanoparticle coated liposomes: A new type of hybrid nanocapsule for proteins. Int J Pharm. 392:285–293. 2010. View Article : Google Scholar : PubMed/NCBI
10	Li X, Chen D, Le C, Zhu C, Gan Y, Hovgaard L and Yang M: Novel mucus-penetrating liposomes as a potential oral drug delivery system: Preparation, in vitro characterization and enhanced cellular uptake. Int J Nanomedicine. 6:3151–3162. 2011.PubMed/NCBI
11	Jiang J, Liu S, Wang C and Zhang H: Overcoming multidrug resistance by on-demand int racellular release of doxorubicin and verapamil. J Nanomater. 2018:72018. View Article : Google Scholar
12	Zhang Y, Huang L, Li Z, Ma G, Zhou Y and Han G: Illuminating cell signaling with near-infrared light-responsive nanomaterials. ACS Nano. 10:3881–3885. 2016. View Article : Google Scholar : PubMed/NCBI
13	Li N, Yu ZZ, Pan W, Han YY, Zhang TT and Tang B: A near-infrared light-triggered nanocarrier with reversible DNA valves for intracellular controlled release. Adv Funct Mater. 23:2255–2262. 2013. View Article : Google Scholar
14	Yavuz MS, Cheng Y, Chen J, Cobley CM, Zhang Q, Rycenga M, Xie J, Kim C, Song KH, Schwartz AG, et al: Gold nanocages covered by smart polymers for controlled release with near-infrared light. Nat Mater. 8:935–939. 2009. View Article : Google Scholar : PubMed/NCBI
15	Weissleder R: A clearer vision for in vivo imaging. Nat Biotechnol. 19:316–317. 2001. View Article : Google Scholar : PubMed/NCBI
16	Li X, Che Z, Mazhar K, Price T and Qin Z: Ultrafast near-infrared light-triggered intracellular uncaging to probe cell signaling. Adv Funct Mater. 27:16057782017. View Article : Google Scholar : PubMed/NCBI
17	Bignami A, Hosley M and Dahl D: Hyaluronic acid and hyaluronic acid-binding proteins in brain extracellular matrix. Anat Embryol (Berl). 188:419–433. 1993. View Article : Google Scholar : PubMed/NCBI
18	Surace C, Arpicco S, Dufay-Wojcicki A, Marsaud V, Bouclier C, Clay D, Cattel L, Renoir JM and Fattal E: Lipoplexes targeting the CD44 hyaluronic acid receptor for efficient transfection of breast cancer cells. Mol Pharm. 6:1062–1073. 2009. View Article : Google Scholar : PubMed/NCBI
19	Oh EJ, Park K, Kim KS, Kim J, Yang JA, Kong JH, Lee MY, Hoffman AS and Hahn SK: Target specific and long-acting delivery of protein, peptide, and nucleotide therapeutics using hyaluronic acid derivatives. J Control Release. 141:2–12. 2010. View Article : Google Scholar : PubMed/NCBI
20	Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF and Ailles LE: Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci USA. 104:973–978. 2007. View Article : Google Scholar : PubMed/NCBI
21	Lee H, Lee K, Kim IK and Park TG: Synthesis, characterization, and in vivo diagnostic applications of hyaluronic acid immobilized gold nanoprobes. Biomaterials. 29:4709–4718. 2008. View Article : Google Scholar : PubMed/NCBI
22	Jiang M, Gan L, Zhu C, Dong Y, Liu J and Gan Y: Cationic core-shell liponanoparticles for ocular gene delivery. Biomaterials. 33:7621–7630. 2012. View Article : Google Scholar : PubMed/NCBI
23	Fomina N, Sankaranarayanan J and Almutairi A: Photochemical mechanisms of light-triggered release from nanocarriers. Adv Drug Deliv Rev. 64:1005–1020. 2012. View Article : Google Scholar : PubMed/NCBI
24	Cheng R, Feng F, Meng F, Deng C, Feijen J and Zhong Z: Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery. J Control Release. 152:2–12. 2011. View Article : Google Scholar : PubMed/NCBI
25	Anderson LJ, Hansen E, Lukianova-Hleb EY, Hafner JH and Lapotko DO: Optically guided controlled release from liposomes with tunable plasmonic nanobubbles. J Control Release. 144:151–158. 2010. View Article : Google Scholar : PubMed/NCBI
26	Fuhrmann G, Serio A, Mazo M, Nair R and Stevens MM: Active loading into extracellular vesicles significantly improves the cellular uptake and photodynamic effect of porphyrins. J Control Release. 205:35–44. 2015. View Article : Google Scholar : PubMed/NCBI