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Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway

  • Authors:
    • Lei Huang
    • Qiong Li
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Jingmen Number 1 People's Hospital, Jingmen, Hubei 448000, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4754-4762
    |
    Published online on: September 25, 2020
       https://doi.org/10.3892/mmr.2020.11537
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Abstract

Alveolar bone is vital for dental implantation and periodontal treatment. Notoginsenoside R1 (NTR1) may promote the differentiation of human alveolar osteoblasts (HAOBs), but the underlying molecular mechanisms remain unclear. The present study investigated the pro‑differentiation function of NTR1 on HAOBs in order to find new methods of dental treatment. HAOBs were surgically obtained from dental patients and the cells were isolated, cultured and identified under an inverted phase contrast microscope. The cells were treated with different concentrations of NTR1 alone or further stimulated by TNF‑α. An alkaline phosphate (ALP) activity assay and alizarin red staining were performed to detect ALP activity and mineralization of the cells, respectively. Cell viability was assayed using an MTT assay. The expressions of osteogenic‑related factors and the factors associated with the NF‑κB and Wnt/β‑catenin pathways were examined by reverse transcription‑quantitative PCR or western blot analysis. Successfully passaged HAOBs presented blue granules and red calcium deposits after staining. The viability of HAOBs was unchanged following treatment with NTR1 at ≤20 µmol/l and/or TNF‑α, but slightly reduced by 40 µmol/l NTR1. TNF‑α‑induced decreases of calcium nodules and ALP activity were decreased by NTR1 in HAOBs. TNF‑α also regulated the expressions of runt‑related transcription factor 2, osteopontin (OPN), osteocalcin (OCN), p50, phosphorylated p65, AXIN2, Dickkopf‑related protein 1 and β‑catenin, while the regulatory effect was reversed by NTR1. NTR1 promoted the differentiation of HAOBs in the TNF‑α‑induced inflammatory microenvironment through inhibiting the NF‑κB pathway and activating the Wnt/β‑catenin pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Huang L and Li Q: Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway. Mol Med Rep 22: 4754-4762, 2020.
APA
Huang, L., & Li, Q. (2020). Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway. Molecular Medicine Reports, 22, 4754-4762. https://doi.org/10.3892/mmr.2020.11537
MLA
Huang, L., Li, Q."Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway". Molecular Medicine Reports 22.6 (2020): 4754-4762.
Chicago
Huang, L., Li, Q."Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway". Molecular Medicine Reports 22, no. 6 (2020): 4754-4762. https://doi.org/10.3892/mmr.2020.11537
Copy and paste a formatted citation
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Spandidos Publications style
Huang L and Li Q: Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway. Mol Med Rep 22: 4754-4762, 2020.
APA
Huang, L., & Li, Q. (2020). Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway. Molecular Medicine Reports, 22, 4754-4762. https://doi.org/10.3892/mmr.2020.11537
MLA
Huang, L., Li, Q."Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway". Molecular Medicine Reports 22.6 (2020): 4754-4762.
Chicago
Huang, L., Li, Q."Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway". Molecular Medicine Reports 22, no. 6 (2020): 4754-4762. https://doi.org/10.3892/mmr.2020.11537
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