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Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats

  • Authors:
    • Xin Xue
    • Liang Zhang
    • Xiang Yin
    • Xing‑Xing Chen
    • Zong‑Feng Chen
    • Chen‑Xu Wang
    • Yu Xiang
    • Ming‑Yong Liu
    • Jian‑Hua Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, Third Military Medical University (Army Medical University), Chongqing 400042, P.R. China, Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, Third Military Medical University (Army Medical University), Chongqing 400042, P.R. China, Department of Orthopedic, No. 517 Hospital of People's Liberation Army, Xinzhou, Shanxi 030002, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4725-4733
    |
    Published online on: October 6, 2020
       https://doi.org/10.3892/mmr.2020.11565
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Abstract

Spinal cord injury (SCI) is a devastating disorder that often results in temporary and/or permanent functional impairment below the injured level. To date, few satisfactory therapeutic strategies are available to treat SCI. Hence, exploring novel strategies for SCI is an essential public health concern. Cell transplantation therapy, which is associated with neuroprotection, immunomodulation, axon regeneration, neuronal relay formation and myelin regeneration, provides a promising therapeutic strategy for SCI. The neuronal stem cell (NSC) preconditioning method is an emerging approach, which facilitates NSC survival and neuronal differentiation after implantation. The aim of the present study was to develop a feasible candidate for cell‑based therapy following SCI in rats and to investigate the role of high mobility group box‑1 (HMGB1) in NSC activation. The results of the present study showed that transplantation of NSCs, preconditioned with 1 ng/ml HMGB1, facilitated functional improvement of injured spinal cords, as indicated by Basso, Beattie and Bresnahan mean scores, mechanical hypersensitivity and cold stimulation. Meanwhile, the histological examination of hematoxylin and eosin staining indicated that engraftment of HMGB1‑preconditioned NSCs resulted in decreased atrophy of the injured spinal cord. Meanwhile, the transplantation of HMGB1‑preconditioned NSCs resulted in an increased number of functional Nissl bodies in neurons, as detected by Nissl staining, and an increase in the number of βIII‑tubulin+ cells in the epicenter of injured spinal cords in rats with SCI. In addition, the results also demonstrated that 1 ng/ml HMGB1 promoted the differentiation of NSCs into neurons, and that the ERK signaling pathway played an important role in this process. In conclusion, the present data indicated that the preconditioning strategy with 1 ng/ml HMGB1 may present a feasible candidate for cell‑based therapy following SCI in rats, which may enlarge the scope of HMGB1 in NSC activation.
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Copy and paste a formatted citation
Spandidos Publications style
Xue X, Zhang L, Yin X, Chen XX, Chen ZF, Wang CX, Xiang Y, Liu MY and Zhao JH: Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats. Mol Med Rep 22: 4725-4733, 2020.
APA
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C. ... Zhao, J. (2020). Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats. Molecular Medicine Reports, 22, 4725-4733. https://doi.org/10.3892/mmr.2020.11565
MLA
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C., Xiang, Y., Liu, M., Zhao, J."Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats". Molecular Medicine Reports 22.6 (2020): 4725-4733.
Chicago
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C., Xiang, Y., Liu, M., Zhao, J."Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats". Molecular Medicine Reports 22, no. 6 (2020): 4725-4733. https://doi.org/10.3892/mmr.2020.11565
Copy and paste a formatted citation
x
Spandidos Publications style
Xue X, Zhang L, Yin X, Chen XX, Chen ZF, Wang CX, Xiang Y, Liu MY and Zhao JH: Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats. Mol Med Rep 22: 4725-4733, 2020.
APA
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C. ... Zhao, J. (2020). Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats. Molecular Medicine Reports, 22, 4725-4733. https://doi.org/10.3892/mmr.2020.11565
MLA
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C., Xiang, Y., Liu, M., Zhao, J."Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats". Molecular Medicine Reports 22.6 (2020): 4725-4733.
Chicago
Xue, X., Zhang, L., Yin, X., Chen, X., Chen, Z., Wang, C., Xiang, Y., Liu, M., Zhao, J."Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats". Molecular Medicine Reports 22, no. 6 (2020): 4725-4733. https://doi.org/10.3892/mmr.2020.11565
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