Open Access

IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway

  • Authors:
    • Shuting Zhu
    • Hong Wang
    • Zhihua Zhang
    • Mingming Ma
    • Zhi Zheng
    • Xun Xu
    • Tao Sun
  • View Affiliations

  • Published online on: October 11, 2020     https://doi.org/10.3892/mmr.2020.11578
  • Pages: 4837-4847
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Insulin‑like growth factor binding protein‑related protein 1 (IGFBP‑rP1) has been reported to have various functions in different cellular contexts. Our previous investigation discovered that IGFBP‑rP1 inhibited retinal angiogenesis in vitro and in vivo by inhibiting the pro‑angiogenic effect of VEGF and downregulating VEGF expression. Recently, IGFBP‑rP1 was confirmed to be downregulated in the aqueous humor of patients with neovascular age‑related macular degeneration compared with controls; however, its specific role remains unknown. The present study applied the technique of gene silencing, reverse transcription‑quantitative PCR, western blotting, cell viability assays, cell motility assays and tube formation assays. Chemical hypoxic conditions and choroidal endothelial (RF/6A) cells were used to explore the effect of IGFBP‑rP1‑silencing on the phenotype activation of RF/6A cells under hypoxic conditions and to elucidate the underlying mechanisms. siRNA achieved IGFBP‑rP1‑silencing in RF/6A cells without cytotoxicity. IGFBP‑rP1‑silencing significantly restored the viability of RF/6A cells in hypoxia and enhanced hypoxia‑induced migration and capillary‑like tube formation of RF/6A cells. Furthermore, IGFBP‑rP1‑silencing significantly upregulated the expression of B‑RAF, phosphorylated (p)‑MEK, p‑ERK and VEGF in RF/6A cells under hypoxic conditions; however, these upregulations were inhibited by exogenous IGFBP‑rP1. These data indicated that silencing IGFBP‑rP1 expression in RF/6A cells effectively promoted the hypoxia‑induced angiogenic potential of choroidal endothelial cells by upregulating RAF/MEK/ERK signaling pathway activation and VEGF expression.
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December-2020
Volume 22 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu S, Wang H, Zhang Z, Ma M, Zheng Z, Xu X and Sun T: IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway. Mol Med Rep 22: 4837-4847, 2020
APA
Zhu, S., Wang, H., Zhang, Z., Ma, M., Zheng, Z., Xu, X., & Sun, T. (2020). IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway. Molecular Medicine Reports, 22, 4837-4847. https://doi.org/10.3892/mmr.2020.11578
MLA
Zhu, S., Wang, H., Zhang, Z., Ma, M., Zheng, Z., Xu, X., Sun, T."IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway". Molecular Medicine Reports 22.6 (2020): 4837-4847.
Chicago
Zhu, S., Wang, H., Zhang, Z., Ma, M., Zheng, Z., Xu, X., Sun, T."IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway". Molecular Medicine Reports 22, no. 6 (2020): 4837-4847. https://doi.org/10.3892/mmr.2020.11578