Open Access

Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis

  • Authors:
    • Yi‑Xin Du
    • Lei Ye
    • Zi‑Jian Song
    • Hui Lv
    • Qian Liu
    • Song‑Guo Li
    • Sheng‑Sheng Liu
    • Jian Hong
    • Yi Gao
    • Marion E. Schneider
    • Wei‑Dong Du
  • View Affiliations

  • Published online on: October 16, 2020     https://doi.org/10.3892/mmr.2020.11605
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hemophagocytic lymphohistiocytosis (HLH) is a rare but severe disease characterized by immune hyperactivation and cytokine storm. Given the high mortality rate of HLH, there is a need for more effective diagnostic tools and treatments. The present study developed a dendrimer‑based protein biochip for rapid, sensitive and simultaneous detection of serum interferon (IFN)‑γ and endogenous anti‑IFN‑γ antibody (Ab) in patients with HLH. A gold biochip was modified with 1, 4‑phenylene diisothiocyanate (PDITC), polyamidoamine (PAMAM) or PDITC‑activated PAMAM. The optimal immobilization concentration for Ab capture and the reaction concentration for detecting Ab on the PDITC‑activated PAMAM‑modified biochip were 6.25 and 3.12 µg/ml, respectively; the limit of detection of IFN‑γ protein was 50 pg/ml. The efficiency of the protein‑probed biochip in detecting IFN‑γ and anti‑IFN‑γ Ab in serum samples from 77 patients with HLH was evaluated; the positive rates for IFN‑γ and anti‑IFN‑γ IgG Ab were 63.6% (49/77) and 61.0% (47/77), respectively. The present results demonstrated that the PDITC‑activated PAMAM‑modified biochip might be a sensitive tool for the specific detection of IFN‑γ and anti‑IFN‑γ Ab in serum, and might have clinical applicability for the diagnosis of HLH.

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Spandidos Publications style
Du YX, Ye L, Song ZJ, Lv H, Liu Q, Li SG, Liu SS, Hong J, Gao Y, Schneider ME, Schneider ME, et al: Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis. Mol Med Rep 0: 0-0, 1899
APA
Du, Y., Ye, L., Song, Z., Lv, H., Liu, Q., Li, S. ... Du, W. (1899). Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis. Molecular Medicine Reports, 0, 0-0. https://doi.org/10.3892/mmr.2020.11605
MLA
Du, Y., Ye, L., Song, Z., Lv, H., Liu, Q., Li, S., Liu, S., Hong, J., Gao, Y., Schneider, M. E., Du, W."Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis". Molecular Medicine Reports 0.0 (1899): 0-0.
Chicago
Du, Y., Ye, L., Song, Z., Lv, H., Liu, Q., Li, S., Liu, S., Hong, J., Gao, Y., Schneider, M. E., Du, W."Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis". Molecular Medicine Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/mmr.2020.11605