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Article Open Access

In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia

  • Authors:
    • Shaohua Cong
    • Qingchun Tong
    • Qian Peng
    • Tao Shen
    • Xueqin Zhu
    • Yuanzhi Xu
    • Shengcai Qi
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Shanghai 10th People's Hospital of Tongji University, Shanghai 200072, P.R. China, Department of Stomatology, Jiading Central Hospital, Shanghai University of Medicine and Health Sciences, Shanghai 201800, P.R. China, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China
    Copyright: © Cong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5392-5398
    |
    Published online on: October 21, 2020
       https://doi.org/10.3892/mmr.2020.11620
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Abstract

Diosgenin (Dios), a natural steroidal sapogenin, is a bioactive compound extracted from dietary fenugreek seeds. It has a wide range of applications, exhibiting anti‑oxidant, anti‑inflammatory and anti‑cancer activities. However, whether the extracts have beneficial effects on periodontal pathogens has so far remained elusive. The aim of the present study was to investigate the anti‑bacterial effects of Dios on Porphyromonas gingivalis (P. gingivalis) and Prevotella intermedia (P. intermedia) in vitro. The anti‑microbial effect of Dios on P. gingivalis and P. intermedia was assessed by a direct contact test (DCT) and the Cell Counting Kit (CCK)‑8 assay at 60, 90 and 120 min. In addition, counting of colony‑forming units (CFU) and live/dead cell staining were used to evaluate the anti‑bacterial effects. The results of the DCT and CCK‑8 assays indicated that Dios had beneficial dose‑dependent inhibitory effects on P. gingivalis and P. intermedia. The CFU counting results also indicated that Dios had dose‑dependent anti‑bacterial effects on P. gingivalis and P. intermedia. Of note, Dios had significant anti‑bacterial effects on the biofilms of P. gingivalis and P. intermedia in vitro as visualized by the live/dead cell staining method. In conclusion, the present results demonstrated that Dios had a marked anti‑bacterial activity against P. gingivalis and P. intermedia in vitro, both in suspension and on biofilms. The present study highlighted the potential applications of Dios as a novel natural agent to prevent and treat periodontitis through its anti‑bacterial effects.
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1 

Page RC and Kornman KS: The pathogenesis of human periodontitis: An introduction. Periodontol 2000. 14:9–11. 1997.PubMed/NCBI

2 

Ravald N and Johansson CS: Tooth loss in periodontally treated patients: A long-term study of periodontal disease and root caries. J Clin Periodontol. 39:73–79. 2012.PubMed/NCBI

3 

Minty M, Canceil T, Serino M, Burcelin R, Terce F and Blasco-Baque V: Oral microbiota-induced periodontitis: A new risk factor of metabolic diseases. Rev Endocr Metab Disord. 20:449–459. 2019.PubMed/NCBI

4 

How KY, Song KP and Chan KG: Porphyromonas gingivalis: An overview of periodontopathic pathogen below the gum line. Front Microbiol. 7:532016.PubMed/NCBI

5 

Reddy PRT, Vandana KV and Prakash S: Antibacterial and anti-inflammatory properties of plantago ovata forssk. Leaves and seeds against periodontal pathogens: An in vitro study. Ayu. 39:226–229. 2018.PubMed/NCBI

6 

Hajishengallis G, Chavakis T, Hajishengallis E and Lambris JD: Neutrophil homeostasis and inflammation: Novel paradigms from studying periodontitis. J Leukoc Biol. 98:539–548. 2015.PubMed/NCBI

7 

Van der Velden U, Abbas F, Armand S, Loos BG, Timmerman MF, Van der Weijden GA, Van Winkelhoff AJ and Winkel EG: Java project on periodontal diseases. The natural development of periodontitis: Risk factors, risk predictors and risk determinants. J Clin Periodontol. 33:540–548. 2006.PubMed/NCBI

8 

Raber-Durlacher JE, van Steenbergen TJ, Van der Velden U, de Graaff J and Abraham-Inpijn L: Experimental gingivitis during pregnancy and post-partum: Clinical, endocrinological, and microbiological aspects. J Clin Periodontol. 21:549–558. 1994.PubMed/NCBI

9 

Kornman KS and Loesche WJ: The subgingival microbial flora during pregnancy. J Periodontal Res. 15:111–122. 1980.PubMed/NCBI

10 

Kolenbrander PE, Palmer RJ Jr, Periasamy S and Jakubovics NS: Oral multispecies biofilm development and the key role of cell-cell distance. Nat Rev Microbiol. 8:471–480. 2010.PubMed/NCBI

11 

Periasamy S and Kolenbrander PE: Mutualistic biofilm communities develop with Porphyromonas gingivalis and initial, early, and late colonizers of enamel. J Bacteriol. 191:6804–6811. 2009.PubMed/NCBI

12 

Socransky SS, Haffajee AD, Cugini MA, Smith C and Kent RL Jr: Microbial complexes in subgingival plaque. J Clin Periodontol. 25:134–144. 1998.PubMed/NCBI

13 

Graziani F, Karapetsa D, Alonso B and Herrera D: Nonsurgical and surgical treatment of periodontitis: How many options for one disease? Periodontol 2000. 75:152–188. 2017.PubMed/NCBI

14 

Qi M, Li X, Sun X, Li C, Tay FR, Weir MD, Dong B, Zhou Y, Wang L and Xu HHK: Novel nanotechnology and near-infrared photodynamic therapy to kill periodontitis-related biofilm pathogens and protect the periodontium. Dent Mater. 35:1665–1681. 2019.PubMed/NCBI

15 

Slots J: Periodontitis: Facts, fallacies and the future. Periodontol 2000. 75:7–23. 2017.PubMed/NCBI

16 

Pretzl B, Sälzer S, Ehmke B, Schlagenhauf U, Dannewitz B, Dommisch H, Eickholz P and Jockel-Schneider Y: Administration of systemic antibiotics during non-surgical periodontal therapy-a consensus report. Clin Oral Investig. 23:3073–3085. 2019.PubMed/NCBI

17 

Moro MG, Silveira Souto ML, Franco GCN, Holzhausen M and Pannuti CM: Efficacy of local phytotherapy in the nonsurgical treatment of periodontal disease: A systematic review. J Periodontal Res. 53:288–297. 2018.PubMed/NCBI

18 

Gunjal S, Ankola AV and Bhat K: In vitro antibacterial activity of ethanolic extract of Morus alba leaf against periodontal pathogens. Indian J Dent Res. 26:533–536. 2015.PubMed/NCBI

19 

Li X, Yu C, Hu Y, Xia X, Liao Y, Zhang J, Chen H, Lu W, Zhou W and Song Z: New application of psoralen and angelicin on periodontitis with anti-bacterial, anti-inflammatory, and osteogenesis effects. Front Cell Infect Microbiol. 8:1782018.PubMed/NCBI

20 

Yang L, Liu X, Zhong L, Sui Y, Quan G, Huang Y, Wang F and Ma T: Dioscin inhibits virulence factors of Candida albicans. Biomed Res Int. 2018:46517262018.PubMed/NCBI

21 

Tikhonova MA, Yu CH, Kolosova NG, Gerlinskaya LA, Maslennikova SO, Yudina AV, Amstislavskaya TG and Ho YJ: Comparison of behavioral and biochemical deficits in rats with hereditary defined or D-galactose-induced accelerated senescence: Evaluating the protective effects of diosgenin. Pharmacol Biochem Behav. 120:7–16. 2014.PubMed/NCBI

22 

Chen Z, Xu J, Wu Y, Lei S, Liu H, Meng Q and Xia Z: Diosgenin inhibited the expression of TAZ in hepatocellular carcinoma. Biochem Biophys Res Commun. 503:1181–1185. 2018.PubMed/NCBI

23 

Sethi G, Shanmugam MK, Warrier S, Merarchi M, Arfuso F, Kumar AP and Bishayee A: Pro-apoptotic and anti-cancer properties of diosgenin: A comprehensive and critical review. Nutrients. 10:6452018.

24 

Tao X, Yin L, Xu L and Peng J: Dioscin: A diverse acting natural compound with therapeutic potential in metabolic diseases, cancer, inflammation and infections. Pharmacol Res. 137:259–269. 2018.PubMed/NCBI

25 

Zand F, Zahed L, Mansouri P, Dehghanrad F, Bahrani M and Ghorbani M: The effects of oral rinse with 0.2 and 2% chlorhexidine on oropharyngeal colonization and ventilator associated pneumonia in adults' intensive care units. J Crit Care. 40:318–322. 2017.PubMed/NCBI

26 

Haydari M, Bardakci AG, Koldsland OC, Aass AM, Sandvik L and Preus HR: Comparing the effect of 0.06 -, 0.12 and 0.2% Chlorhexidine on plaque, bleeding and side effects in an experimental gingivitis model: A parallel group, double masked randomized clinical trial. BMC Oral Health. 17:1182017.PubMed/NCBI

27 

Kinane DF, Stathopoulou PG and Papapanou PN: Periodontal diseases. Nat Rev Dis Primers. 3:170382017.PubMed/NCBI

28 

Selim S and Al Jaouni S: Anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm. Nat Prod Res. 30:1830–1833. 2016.PubMed/NCBI

29 

Chen Y, Tang YM, Yu SL, Han YW, Kou JP, Liu BL and Yu BY: Advances in the pharmacological activities and mechanisms of diosgenin. Chin J Nat Med. 13:578–587. 2015.PubMed/NCBI

30 

Kiasalari Z, Rahmani T, Mahmoudi N, Baluchnejadmojarad T and Roghani M: Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation. Biomed Pharmacother. 86:654–661. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Wu L, Dong H, Zhao J, Wang Y, Yang Q, Jia C and Ma J: Diosgenin stimulates rat TM4 cell proliferation through activating plasma membrane translocation and transcriptional activity of estrogen receptors. Biol Reprod. 92:242015. View Article : Google Scholar : PubMed/NCBI

32 

Huang CH, Liu DZ and Jan TR: Diosgenin, a plant-derived sapogenin, enhances regulatory T-cell immunity in the intestine of mice with food allergy. J Nat Prod. 73:1033–1037. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Cai B, Seong KJ, Bae SW, Chun C, Kim WJ and Jung JY: A synthetic diosgenin primary amine derivative attenuates LPS-stimulated inflammation via inhibition of NF-κB and JNK MAPK signaling in microglial BV2 cells. Int Immunopharmacol. 61:204–214. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Choi KW, Park HJ, Jung DH, Kim TW, Park YM, Kim BO, Sohn EH, Moon EY, Um SH, Rhee DK, et al: Inhibition of TNF-α-induced adhesion molecule expression by diosgenin in mouse vascular smooth muscle cells via downregulation of the MAPK, Akt and NF-κB signaling pathways. Vascul Pharmacol. 53:273–280. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Tharaheswari M, Jayachandra Reddy N, Kumar R, Varshney KC, Kannan M and Sudha Rani S: Trigonelline and diosgenin attenuate ER stress, oxidative stress-mediated damage in pancreas and enhance adipose tissue PPARγ activity in type 2 diabetic rats. Mol Cell Biochem. 396:161–174. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Tao X, Qi Y, Xu L, Yin L, Han X, Xu Y, Wang C, Sun H and Peng J: Dioscin reduces ovariectomy-induced bone loss by enhancing osteoblastogenesis and inhibiting osteoclastogenesis. Pharmacol Res. 108:90–101. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Srinivasan S, Koduru S, Kumar R, Venguswamy G, Kyprianou N and Damodaran C: Diosgenin targets Akt-mediated prosurvival signaling in human breast cancer cells. Int J Cancer. 125:961–967. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Guo N, Tong T, Ren N, Tu Y and Li B: Saponins from seeds of genus Camellia: Phytochemistry and bioactivity. Phytochemistry. 149:42–55. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Hu K, Berenjian S, Larsson R, Gullbo J, Nygren P, Lövgren T and Morein B: Nanoparticulate Quillaja saponin induces apoptosis in human leukemia cell lines with a high therapeutic index. Int J Nanomedicine. 5:51–62. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Cai BX, Jin SL, Luo D, Lin XF and Gao J: Ginsenoside Rb1 suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair. Biol Pharm Bull. 32:837–841. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Balestrazzi A, Agoni V, Tava A, Avato P, Biazzi E, Raimondi E, Macovei A and Carbonera D: Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins. Physiol Plant. 141:227–238. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Zhang L and Wei W: Anti-inflammatory and immunoregulatory effects of paeoniflorin and total glucosides of paeony. Pharmacol Ther. 207:1074522020. View Article : Google Scholar : PubMed/NCBI

43 

Minami M, Takase H, Taira M and Makino T: In Vitro Effect of the Traditional Medicine Hainosan (Painongsan) on Porphyromonas gingivalis. Medicines (Basel). 6:582019. View Article : Google Scholar

44 

Gentil M, Pereira JV, Sousa YT, Pietro R, Neto MD, Vansan LP and de Castro França S: In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings. Phytother Res. 20:184–186. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cong S, Tong Q, Peng Q, Shen T, Zhu X, Xu Y and Qi S: In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia. Mol Med Rep 22: 5392-5398, 2020.
APA
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., & Qi, S. (2020). In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia. Molecular Medicine Reports, 22, 5392-5398. https://doi.org/10.3892/mmr.2020.11620
MLA
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., Qi, S."In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia". Molecular Medicine Reports 22.6 (2020): 5392-5398.
Chicago
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., Qi, S."In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia". Molecular Medicine Reports 22, no. 6 (2020): 5392-5398. https://doi.org/10.3892/mmr.2020.11620
Copy and paste a formatted citation
x
Spandidos Publications style
Cong S, Tong Q, Peng Q, Shen T, Zhu X, Xu Y and Qi S: In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia. Mol Med Rep 22: 5392-5398, 2020.
APA
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., & Qi, S. (2020). In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia. Molecular Medicine Reports, 22, 5392-5398. https://doi.org/10.3892/mmr.2020.11620
MLA
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., Qi, S."In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia". Molecular Medicine Reports 22.6 (2020): 5392-5398.
Chicago
Cong, S., Tong, Q., Peng, Q., Shen, T., Zhu, X., Xu, Y., Qi, S."In vitro anti‑bacterial activity of diosgenin on Porphyromonas gingivalis and Prevotella intermedia". Molecular Medicine Reports 22, no. 6 (2020): 5392-5398. https://doi.org/10.3892/mmr.2020.11620
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