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Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans

  • Authors:
    • Baohong Jiang
    • Rui Wang
    • Zefei Lin
    • Jieyi Ma
    • Jin Cui
    • Mian Wang
    • Ruiming  Liu
    • Weibin Wu
    • Chunxiang Zhang
    • Wen Li
    • Shenming Wang
  • View Affiliations / Copyright

    Affiliations: Division of Vascular Surgery, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Laboratory of General Surgery, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, National‑Local Joint Engineering Laboratory of Vascular Disease Diagnosis and Treatment, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35233, USA
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5069-5082
    |
    Published online on: October 22, 2020
       https://doi.org/10.3892/mmr.2020.11625
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Abstract

Long non‑coding RNAs (lncRNAs) affect atherosclerosis by regulating the physiological and pathological processes of endothelial cells; however, the role of lncRNA WEE2 antisense RNA 1 (WEE2‑AS1) in arteriosclerosis obliterans (ASO) is not completely understood. The present study aimed to explore the function of lncRNA WEE2‑AS1 in human vascular endothelial cells. The results indicated that lncRNA WEE2‑AS1 was significantly elevated in plasma and artery tissue samples of patients with ASO compared with healthy controls. The fluorescence in situ hybridization results suggested that lncRNA WEE2‑AS1 was expressed in the cytoplasm and nuclei of primary human umbilical vein endothelial cells (HUVECs). The Cell Counting Kit‑8 assay results suggested that lncRNA WEE2‑AS1 knockdown significantly promoted HUVEC viability, whereas lncRNA WEE2‑AS1 overexpression inhibited HUVEC viability compared with the negative control groups. Furthermore, analysis of the cell cycle by flow cytometry indicated that lncRNA WEE2‑AS1 knockdown significantly decreased the proportion of cells in the G0/G1 phase and significantly increased the proportion of cells in the G2/M phase compared with the negative control group. However, lncRNA WEE2‑AS1 overexpression had no significant effect on cell cycle distribution compared with the negative control group. The western blotting results indicated that lncRNA WEE2‑AS1 knockdown significantly reduced the expression levels of phosphorylated cyclin dependent kinase 1, WEE1 homolog 2 and myelin transcription factor 1, but increased the expression level of cell division cycle 25B compared with the negative control group. lncRNA WEE2‑AS1 overexpression displayed the opposite effect on protein expression. Collectively, the present study suggested that lncRNA WEE2‑AS1 was significantly upregulated in ASO and may serve a role in regulating human vascular endothelial cell viability. Further investigation into lncRNA WEE2‑AS1 may broaden the current understanding of the molecular mechanism underlying ASO, and aid with the identification of specific probes and precise targeted drugs for the diagnosis and treatment of ASO.
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Spandidos Publications style
Jiang B, Wang R, Lin Z, Ma J, Cui J, Wang M, Liu R, Wu W, Zhang C, Li W, Li W, et al: Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans. Mol Med Rep 22: 5069-5082, 2020.
APA
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M. ... Wang, S. (2020). Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans. Molecular Medicine Reports, 22, 5069-5082. https://doi.org/10.3892/mmr.2020.11625
MLA
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M., Liu, R., Wu, W., Zhang, C., Li, W., Wang, S."Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans". Molecular Medicine Reports 22.6 (2020): 5069-5082.
Chicago
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M., Liu, R., Wu, W., Zhang, C., Li, W., Wang, S."Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans". Molecular Medicine Reports 22, no. 6 (2020): 5069-5082. https://doi.org/10.3892/mmr.2020.11625
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang B, Wang R, Lin Z, Ma J, Cui J, Wang M, Liu R, Wu W, Zhang C, Li W, Li W, et al: Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans. Mol Med Rep 22: 5069-5082, 2020.
APA
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M. ... Wang, S. (2020). Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans. Molecular Medicine Reports, 22, 5069-5082. https://doi.org/10.3892/mmr.2020.11625
MLA
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M., Liu, R., Wu, W., Zhang, C., Li, W., Wang, S."Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans". Molecular Medicine Reports 22.6 (2020): 5069-5082.
Chicago
Jiang, B., Wang, R., Lin, Z., Ma, J., Cui, J., Wang, M., Liu, R., Wu, W., Zhang, C., Li, W., Wang, S."Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans". Molecular Medicine Reports 22, no. 6 (2020): 5069-5082. https://doi.org/10.3892/mmr.2020.11625
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