Open Access

Inhibition of FOXO1‑mediated autophagy promotes paclitaxel‑induced apoptosis of MDA‑MB‑231 cells

  • Authors:
    • Kaixiang Xu
    • Wanyun Zhu
    • Anyong Xu
    • Zhe Xiong
    • Di Zou
    • Heng Zhao
    • Deling Jiao
    • Yubo Qing
    • Muhammad Ameen Jamal
    • Hong-Jiang Wei
    • Hong-Ye Zhao
  • View Affiliations

  • Published online on: January 5, 2022     https://doi.org/10.3892/mmr.2022.12588
  • Article Number: 72
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Triple‑negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and it often becomes resistant to paclitaxel (PTX) therapy. Autophagy plays an important cytoprotective role in PTX‑induced tumor cell death, and targeting autophagy has been promising for improving the efficacy of tumor chemotherapy in recent years. The aim of the present study was to clarify the mechanism of PTX inducing autophagy in TNBC cells to provide a potential clinical chemotherapy strategy of PTX for TNBC. The present study reported that PTX induced both apoptosis and autophagy in MDA‑MB‑231 cells and that inhibition of autophagy promoted apoptotic cell death. Furthermore, it was found that forkhead box transcription factor O1 (FOXO1) enhanced PTX‑induced autophagy through a transcriptional activation pattern in MDA‑MB‑231 cells, which was associated with the downstream target genes autophagy related 5, class III phosphoinositide 3‑kinase vacuolar protein sorting 34, autophagy related 4B cysteine peptidase, beclin 1 and microtubule associated protein 1 light chain 3β. Knocking down FOXO1 attenuated the survival of MDA‑MB‑231 cells in response to PTX treatment. These findings may be beneficial for improving the treatment efficacy of PTX and to develop autophagic targeted therapy for TNBC.

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February-2022
Volume 25 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Xu K, Zhu W, Xu A, Xiong Z, Zou D, Zhao H, Jiao D, Qing Y, Jamal MA, Wei H, Wei H, et al: Inhibition of FOXO1‑mediated autophagy promotes paclitaxel‑induced apoptosis of MDA‑MB‑231 cells. Mol Med Rep 25: 72, 2022
APA
Xu, K., Zhu, W., Xu, A., Xiong, Z., Zou, D., Zhao, H. ... Zhao, H. (2022). Inhibition of FOXO1‑mediated autophagy promotes paclitaxel‑induced apoptosis of MDA‑MB‑231 cells. Molecular Medicine Reports, 25, 72. https://doi.org/10.3892/mmr.2022.12588
MLA
Xu, K., Zhu, W., Xu, A., Xiong, Z., Zou, D., Zhao, H., Jiao, D., Qing, Y., Jamal, M. A., Wei, H., Zhao, H."Inhibition of FOXO1‑mediated autophagy promotes paclitaxel‑induced apoptosis of MDA‑MB‑231 cells". Molecular Medicine Reports 25.2 (2022): 72.
Chicago
Xu, K., Zhu, W., Xu, A., Xiong, Z., Zou, D., Zhao, H., Jiao, D., Qing, Y., Jamal, M. A., Wei, H., Zhao, H."Inhibition of FOXO1‑mediated autophagy promotes paclitaxel‑induced apoptosis of MDA‑MB‑231 cells". Molecular Medicine Reports 25, no. 2 (2022): 72. https://doi.org/10.3892/mmr.2022.12588