Open Access

NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation

  • Authors:
    • Ye Kuang
    • Chuanmei Peng
    • Yulin Dong
    • Jia Wang
    • Fanbin Kong
    • Xiaoqing Yang
    • Yang Wang
    • Hui Gao
  • View Affiliations

  • Published online on: April 14, 2022     https://doi.org/10.3892/mmr.2022.12711
  • Article Number: 195
  • Copyright: © Kuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myeloid leukemia (AML) is a type of hematological malignancy caused by uncontrolled clonal proliferation of hematopoietic stem cells. The special energy metabolism mode of AML relying on oxidative phosphorylation is different from the traditional ‘Warburg effect’. However, its mechanism is not clear. In the present study, it was demonstrated that the mRNA expression levels of NADH dehydrogenase subunit 1, 4 and 5 (ND1, ND4 and ND5) were upregulated in AML samples from The Cancer Genome Atlas database using the limma package in the R programming language. Reverse transcription‑quantitative PCR and ELISA were used to verify the upregulation of ND1, ND4 and ND5 in clinical samples. Pan‑cancer analysis revealed that the expression of ND1 was upregulated only in AML, ND2 was upregulated only in AML and thymoma, and ND4 was upregulated only in AML and kidney chromophobe. In the present study, it was demonstrated that silencing of ND1/4/5 could inhibit the proliferation of AML cells in transplanted tumor of nude mice. Additionally, it was found that oxidative phosphorylation and energy metabolism of AML cells were decreased after silencing of ND1/4/5. In conclusion, the present study suggested that ND1/4/5 may be involved in the regulation of oxidative phosphorylation metabolism in AML as a potential cancer‑promoting factor.
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June-2022
Volume 25 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kuang Y, Peng C, Dong Y, Wang J, Kong F, Yang X, Wang Y and Gao H: NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation. Mol Med Rep 25: 195, 2022.
APA
Kuang, Y., Peng, C., Dong, Y., Wang, J., Kong, F., Yang, X. ... Gao, H. (2022). NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation. Molecular Medicine Reports, 25, 195. https://doi.org/10.3892/mmr.2022.12711
MLA
Kuang, Y., Peng, C., Dong, Y., Wang, J., Kong, F., Yang, X., Wang, Y., Gao, H."NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation". Molecular Medicine Reports 25.6 (2022): 195.
Chicago
Kuang, Y., Peng, C., Dong, Y., Wang, J., Kong, F., Yang, X., Wang, Y., Gao, H."NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation". Molecular Medicine Reports 25, no. 6 (2022): 195. https://doi.org/10.3892/mmr.2022.12711