[Corrigendum] Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway

Figure 3.

BITC inhibits the migration, invasion and metastasis of 4T1-Luc cells. (A) BITC inhibited the migration of 4T1-Luc cells. 4T1-Luc cells were treated with BITC as indicated and subjected to a wound healing assay (×100). (B) BITC inhibited the migration and invasion of murine mammary carcinoma cells. 4T1-Luc cells were cultured in Transwell chambers coated with or without Matrigel for 24 h, in the presence of the indicated concentrations of BITC (×40). (C) BITC inhibited cellular migration by regulating the expression levels of vimentin and E-cadherin. 4T1-Luc cells were treated with various concentrations of BITC as indicated for 24 and 48 h. Total protein lysates were immunoblotted for E-cadherin and vimentin expression. β-actin was used as the internal control. (D) Inhibitory effects of BITC on the metastasis of murine mammary carcinoma in mice was monitored using an optical in vivo bioluminescence imaging system every 7 days (n=8 mice/group). (E) Quantification of radiance recorded from the tumors were analyzed. **P<0.01 vs. C (21 days); ##P<0.01 vs. C (28 days); ∆∆P<0.01 vs. C (35 days). (F) Tumor sections were assessed via immunohistochemistry to determine the expression of E-cadherin and vimentin. The expression of E-cadherin was upregulated, whereas that of vimentin was downregulated in BITC-treated mice compared with those in the control group (magnification, ×400). BITC, benzyl isothiocyanate; C, control; DDP, cisplatin.